公开(公告)号：US20170268030A1

公开(公告)日：2017-09-21

申请号：US15611803

申请日：2017-06-02

申请人： Yeda Research and Development Co. Ltd.

发明人： Ron MILO ,  Lior ZELCBUCH ,  Niv ANTONOVSKY

IPC分类号： C12P23/00 ,  C12N15/70 ,  C12N9/90 ,  C12N15/52

CPC分类号： C12P23/00 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/13129 ,  C12Y205/01 ,  C12Y205/01029 ,  C12Y505/01019

摘要： A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.

公开(公告)号：US09765308B2

公开(公告)日：2017-09-19

申请号：US14510232

申请日：2014-10-09

申请人： National Research Council of Canada ,  University of Saskatchewan

发明人： Jonathan E. Page ,  Zakia Boubakir

IPC分类号： C12P17/06 ,  C12N15/82 ,  C12N9/10 ,  C12P7/22 ,  C12P7/42

CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12Y205/01

摘要： Nucleic acid molecules from cannabis has been isolated and characterized and encode polypeptides having aromatic prenyltransferase activity. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.

公开(公告)号：US09719122B2

公开(公告)日：2017-08-01

申请号：US14415612

申请日：2013-07-17

申请人： Yeda Research and Development Co. Ltd.

发明人： Ron Milo ,  Lior Zelcbuch ,  Niv Antonovsky

IPC分类号： C12P23/00 ,  C12N15/52 ,  C12N15/70 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12N15/67

CPC分类号： C12P23/00 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/13129 ,  C12Y205/01 ,  C12Y205/01029 ,  C12Y505/01019

摘要： A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.

公开(公告)号：US20160194674A1

公开(公告)日：2016-07-07

申请号：US14400346

申请日：2013-05-13

申请人： Teknologian tutkimuskeskus VTT

发明人： Kirsi Bromann ,  Mervi Toivari ,  Tiina Nakari-Setälä ,  Laura Ruohonen

IPC分类号： C12P15/00 ,  C12P5/00 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04 ,  C12N15/52 ,  C12N9/10

CPC分类号： C12P15/00 ,  C12N9/0006 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/80 ,  C12P5/007 ,  C12Y101/01034 ,  C12Y205/01 ,  C12Y205/01021 ,  C12Y402/03017 ,  C12Y402/03046 ,  C12Y402/03114 ,  C12Y505/01013

摘要： The present invention concerns a method for producing terpenes in fungi comprising the steps of (a) providing a modified terpene biosynthetic gene cluster inside a host cell, wherein one or more of the naturally occurring genes or promoters of the cluster have been replaced, truncated or removed, (b) providing a transcription factor inside the host cell, the transcription factor activating the terpene biosynthetic gene cluster; (c) cultivating said host in conditions allowing the expression of the transcription factor activating the cluster; and optionally (d) recovering the thus produced terpene product.

摘要翻译： 本发明涉及一种在真菌中制备萜烯的方法，包括以下步骤：（a）在宿主细胞内提供修饰的萜烯生物合成基因簇，其中簇的一个或多个天然存在的基因或启动子被替换，截短或 去除，（b）在宿主细胞内提供转录因子，激活萜烯生物合成基因簇的转录因子; （c）在允许表达启动簇的转录因子的条件下培养所述宿主; 和（d）回收由此制得的萜烯产物。

公开(公告)号：US08993305B2

公开(公告)日：2015-03-31

申请号：US13646562

申请日：2012-10-05

申请人： Danisco US Inc. ,  The Goodyear Tire & Rubber Company

发明人： Zachary Q. Beck ,  Andrew C. Eliot ,  Caroline M. Peres ,  Dmitrii V. Vaviline

IPC分类号： C12N1/21 ,  C12P5/00 ,  C12N15/70 ,  C12N9/88

CPC分类号： C12P5/007 ,  C12N1/12 ,  C12N1/14 ,  C12N1/20 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/70 ,  C12P7/42 ,  C12Y203/01008 ,  C12Y205/01 ,  C12Y401/02009 ,  C12Y402/03027 ,  C12Y503/03002 ,  Y02P20/52

摘要： The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.

摘要翻译： 本发明提供了通过异源表达磷酸酮糖醇酶在细胞中生产甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的方法。

公开(公告)号：US08884100B2

公开(公告)日：2014-11-11

申请号：US13389815

申请日：2010-08-04

申请人： Jonathan E. Page ,  Zakia Boubakir

发明人： Jonathan E. Page ,  Zakia Boubakir

IPC分类号： C12N15/82 ,  C07H21/04 ,  C07K14/415 ,  C12N15/74 ,  C12P17/06 ,  C12N9/10

CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12Y205/01

摘要： Nucleic acid molecules from Cannabis sativa (cannabis, hemp, marijuana) have been isolated and characterized, and encode polypeptides having aromatic prenyltransferase activity. Specifically, the enzyme, CsPT1, is a geranylpyrophosphate olivetolate geranyltransferase, active in the cannabinoid biosynthesis step of prenylation of olivetolic acid to form cannabigerolic acid (CBGA). Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.

摘要翻译： 已经分离并表征了大麻（大麻，大麻，大麻）的核酸分子，并编码具有芳族异戊烯基转移酶活性的多肽。 具体地说，酶CsPT1是一种杀稗草酰焦磷酸酯橄榄油醇甘油转移酶，在大肠杆菌生物合成步骤中活化橄榄油酸，以形成大麻酚酸（CBGA）。 核酸的表达或过表达改变大麻素化合物的水平。 多肽可以在体内或体外用于产生大麻素化合物。

公开(公告)号：US12084704B2

公开(公告)日：2024-09-10

申请号：US16612065

申请日：2018-04-19

申请人： Global Bioenergies

发明人： Romain Chayot ,  Mathieu Allard ,  Maria Anissimova

IPC分类号： C12P5/02

CPC分类号： C12P5/026 ,  C12Y205/01 ,  C12Y207/0105 ,  C12Y207/04026 ,  C12Y207/06002 ,  C12Y207/06003 ,  C12Y401/01 ,  C12Y503/03002

摘要： Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl phosphate (DMAP) into a flavin-derived cofactor, wherein said method further comprises providing said DMAP enzymatically by: (i) the enzymatic conversion of dimethylallyl pyrophosphate (DMAPP) into said DMAP; or (ii) a single enzymatic step in which prenol is directly enzymatically converted into said DMAP; or (iii) two enzymatic steps comprising: first enzymatically converting DMAPP into prenol; and then enzymatically converting the thus obtained prenol into said DMAP; or (iv) the enzymatic conversion of isopentenyl monophosphate (IMP) into said DMAP, or by a combination of any one of (i) to (iv). Moreover, described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein the enzymatic conversion of 3-methylcrotonic acid into isobutene is achieved by making use of an FMN-dependent decarboxylase associated with an FMN prenyl transferase, wherein said FMN prenyl transferase catalyzes the prenylation of a flavin cofactor (FMN or FAD) utilizing dimethylallyl pyrophosphate (DMAPP), wherein said method further comprises providing said DMAPP enzymatically by: (v) the enzymatic conversion of isopentenyl pyrophosphate (IPP) into said DMAPP; or (vi) the enzymatic conversion of dimethylallyl phosphate (DMAP) into said DMAPP; or (vii) the enzymatic conversion of prenol into said DMAPP; (viii) or by a combination of any one of (v) to (vii). Moreover, described are methods for providing said flavin cofactor enzymatically by the enzymatic conversion of riboflavin into flavin mononucleotide (FMN).

公开(公告)号：US20240279695A1

公开(公告)日：2024-08-22

申请号：US17298464

申请日：2019-12-03

申请人： The Regents of the University of California

发明人： Bradley S. Moore ,  Jonathan R. Chekan ,  Shaun M. K. McKinnie

IPC分类号： C12P17/18 ,  C12N9/02 ,  C12N9/10

CPC分类号： C12P17/188 ,  C12N9/0071 ,  C12N9/1085 ,  C12Y114/11 ,  C12Y205/01

摘要： Provided herein are compounds of Formulas (la), (lb), (II) to (VIII), and salts thereof, and methods of making the same. Also provided herein are recombinant proteins useful in the production of compounds disclosed herein, polynucleotides encoding the same, and cells comprising the same.

公开(公告)号：US20230212623A1

公开(公告)日：2023-07-06

申请号：US17754988

申请日：2020-10-28

申请人： CJ CHEILJEDANG CORPORATION

发明人： Sol CHOI ,  Jin Nam LEE ,  Hee Ju KIM ,  Jin Ah RHO ,  Han Hyoung LEE

IPC分类号： C12P13/12 ,  C12N15/77 ,  C12N9/10

CPC分类号： C12P13/12 ,  C12N9/1085 ,  C12N15/77 ,  C12Y205/01 ,  C12N2800/101

摘要： An L-methionine-producing microorganism into which a metZ gene is introduced and a method of producing L-methionine using the same.

公开(公告)号：US20180080031A1

公开(公告)日：2018-03-22

申请号：US15560951

申请日：2016-03-23

申请人： ARCH INNOTEK, LLC

发明人： Yechun WANG

IPC分类号： C12N15/52 ,  C12N9/90 ,  C12N9/02 ,  C12N9/10 ,  C12P5/02 ,  C12P7/26 ,  C12N15/70 ,  C12N15/81

CPC分类号： C12N15/52 ,  C12N9/001 ,  C12N9/0069 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/815 ,  C12P5/026 ,  C12P7/26 ,  C12P23/00 ,  C12Y101/01034 ,  C12Y103/99 ,  C12Y113/11051 ,  C12Y205/01 ,  C12Y205/01001 ,  C12Y205/0101 ,  C12Y505/01018 ,  C12Y505/01019

摘要： The present invention relates to compositions and methods of producing carotenoids and carotenoid derivatives.