公开(公告)号：US20150211040A1

公开(公告)日：2015-07-30

申请号：US14415612

申请日：2013-07-17

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron Milo ,  Lior Zelcbuch ,  Niv Antonovsky

IPC: C12P23/00 ,  C12N15/52 ,  C12N9/10 ,  C12N9/90 ,  C12N9/02

CPC classification number: C12P23/00 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/13129 ,  C12Y205/01 ,  C12Y205/01029 ,  C12Y505/01019

Abstract: A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.

Abstract translation: 公开了编码虾青素途径酶的多个分离的多核苷酸序列。 多核苷酸包括：（i）编码叶绿素脱氢酶（crtI）和第一转录调控序列的多核苷酸; （ii）编码β-番茄红素环化酶（lcy-B）和第二转录调控序列的多核苷酸; （iii）编码β-胡萝卜素酮醇酶（crtW）和第三转录调节序列的多核苷酸; 并且其中选择第一，第二和第三调节序列使得Icy-B和crtW的表达大于crtI的表达水平。 还公开了使用多种多核苷酸产生虾青素的方法以及包含高水平的虾青素的细菌细胞。

公开(公告)号：US10155933B2

公开(公告)日：2018-12-18

申请号：US14417559

申请日：2013-07-29

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Arren Bar-Even ,  Ron Milo ,  Elad Noor ,  Oren Yishai

IPC: C12N9/02 ,  C12N9/00 ,  C12N15/52 ,  C12P7/40 ,  C12M1/42 ,  C12N9/06 ,  C12N9/78 ,  C12N13/00

Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.

公开(公告)号：US20190032027A1

公开(公告)日：2019-01-31

申请号：US16162427

申请日：2018-10-17

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Arren Bar-Even ,  Ron Milo ,  Elad Noor ,  Oren Yishai

IPC: C12N9/02 ,  C12N15/52 ,  C12N13/00 ,  C12N9/06 ,  C12P7/40 ,  C12M1/42 ,  C12N9/00 ,  C12N9/78

CPC classification number: C12N9/0008 ,  C12M35/02 ,  C12N9/0028 ,  C12N9/78 ,  C12N9/93 ,  C12N13/00 ,  C12N15/52 ,  C12P7/40 ,  C12Y102/01002 ,  C12Y105/01005 ,  C12Y105/01015 ,  C12Y305/04009 ,  C12Y603/04003

Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.

公开(公告)号：US09719122B2

公开(公告)日：2017-08-01

申请号：US14415612

申请日：2013-07-17

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron Milo ,  Lior Zelcbuch ,  Niv Antonovsky

IPC: C12P23/00 ,  C12N15/52 ,  C12N15/70 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12N15/67

CPC classification number: C12P23/00 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/0073 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/67 ,  C12N15/70 ,  C12Y114/13129 ,  C12Y205/01 ,  C12Y205/01029 ,  C12Y505/01019

Abstract: A plurality of isolated polynucleotide sequences encoding enzymes of the astaxanthin pathway is disclosed. The polynucleotides include: (i) a polynucleotide which encodes Phytoene dehydrogenase (crtI) and a first transcriptional regulatory sequence; (ii) a polynucleotide which encodes Beta-lycopene cyclase (lcy-B) and a second transcriptional regulatory sequence; (iii) a polynucleotide which encodes Beta-carotene ketolase (crtW) and a third transcriptional regulatory sequence; and wherein the first, second and third regulatory sequence are selected such that the expression of the Icy-B and the crtW is greater than a level of expression of the crtI. Methods of generating astaxanthin using the plurality of polynucleotide are also disclosed as well as bacterial cells comprising high levels of astaxanthin.

公开(公告)号：US12031138B2

公开(公告)日：2024-07-09

申请号：US16362897

申请日：2019-03-25

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron Milo ,  Niv Antonovsky ,  Elad Noor ,  Arren Bar-Even ,  Yehudit Zohar ,  Lior Zelcbuch ,  Shmuel Gleizer ,  Shira Amram

IPC: C12N15/70 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90

CPC classification number: C12N15/70 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/1205 ,  C12N9/88 ,  C12N9/90 ,  C12Y101/01049 ,  C12Y203/03009 ,  C12Y207/01011 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y402/01011 ,  C12Y504/02

Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.

公开(公告)号：US10377994B2

公开(公告)日：2019-08-13

申请号：US16162427

申请日：2018-10-17

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Arren Bar-Even ,  Ron Milo ,  Elad Noor ,  Oren Yishai

IPC: C12N9/88 ,  C12N9/04 ,  C12N15/52 ,  C12P7/64 ,  C12N9/02 ,  C12N9/00 ,  C12P7/40 ,  C12M1/42 ,  C12N9/06 ,  C12N9/78 ,  C12N13/00

Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.

公开(公告)号：US10053712B2

公开(公告)日：2018-08-21

申请号：US15101097

申请日：2014-12-10

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Arren Bar-Even ,  Ron Milo ,  Elad Noor ,  Lior Zelcbuch

IPC: C12P7/40 ,  C12N1/20 ,  C12N9/10 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/06 ,  C12P7/16 ,  C12P7/26 ,  C12P7/64

CPC classification number: C12P7/40 ,  C12N1/20 ,  C12N9/1029 ,  C12N15/52 ,  C12P5/007 ,  C12P5/023 ,  C12P5/026 ,  C12P7/06 ,  C12P7/16 ,  C12P7/26 ,  C12P7/649 ,  C12Y203/01054 ,  Y02E50/17 ,  Y02E50/343

Abstract: An isolated microorganism is disclosed being genetically modified to express pyruvate formate lyase (PFL) or 2-ketobutyrate formate lyase, wherein acetyl-CoA of the microorganism is converted to pyruvate in the presence of formate in a single step reaction, wherein the net flux of the reaction is in the direction of pyruvate synthesis.Uses of the microorganism and products comprising same are also disclosed.

公开(公告)号：US10294482B2

公开(公告)日：2019-05-21

申请号：US15312734

申请日：2015-05-21

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron Milo ,  Niv Antonovsky ,  Elad Noor ,  Arren Bar-Even ,  Yehudit Zohar ,  Lior Zelcbuch ,  Shmuel Gleizer ,  Shira Amram

IPC: C12N15/70 ,  C12N15/52 ,  C12N15/74 ,  C07K14/195 ,  C07K14/245 ,  C12N1/20 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90

Abstract: A microorganism which is genetically modified so that it produces a first essential biomass precursor by metabolizing CO2 using a recombinant carbon fixation enzyme is disclosed. The microorganism produces a second biomass precursor by metabolizing an organic carbon source and not by metabolizing CO2. The microorganism does not use the organic carbon source for producing the first essential biomass precursor.

公开(公告)号：US10077437B2

公开(公告)日：2018-09-18

申请号：US15189021

申请日：2016-06-22

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Ron Milo ,  Arren Bar-Even ,  Elad Noor

IPC: C12N9/00 ,  C12N15/82 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/10 ,  C12N9/18 ,  C12N9/88 ,  C12N9/90 ,  C12P7/40 ,  C12N15/70 ,  C12P7/42 ,  C12P7/46 ,  C12P11/00 ,  G06F19/12

CPC classification number: C12N9/93 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/18 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8269 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P11/00 ,  C12Y101/01059 ,  C12Y102/01018 ,  C12Y201/03001 ,  C12Y208/03001 ,  C12Y401/01031 ,  C12Y402/01017 ,  C12Y402/01054 ,  C12Y604/01001 ,  C12Y604/01002 ,  G16B5/00 ,  Y02P20/52

Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.

公开(公告)号：US20150218528A1

公开(公告)日：2015-08-06

申请号：US14417559

申请日：2013-07-29

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Arren Bar-Even ,  Ron Milo ,  Elad Noor ,  Oren Yishai

IPC: C12N9/02 ,  C12M1/42 ,  C12N9/06 ,  C12N13/00 ,  C12N9/00 ,  C12N9/78

CPC classification number: C12N9/0008 ,  C12M35/02 ,  C12N9/0028 ,  C12N9/78 ,  C12N9/93 ,  C12N13/00 ,  C12N15/52 ,  C12P7/40 ,  C12Y102/01002 ,  C12Y105/01005 ,  C12Y105/01015 ,  C12Y305/04009 ,  C12Y603/04003

Abstract: An isolated microorganism that expresses enzymes of the reductive glycine pathway is disclosed. The microorganism is capable of converting formate to pyruvate or glycerate via the formation of glycine and serine. Methods of generating same are further described.

Abstract translation: 公开了表达还原甘氨酸途径的酶的分离的微生物。 微生物能够通过形成甘氨酸和丝氨酸将甲酸转化成丙酮酸或甘油酸。 进一步描述生成方法。