公开(公告)号：US11866763B2

公开(公告)日：2024-01-09

申请号：US16684056

申请日：2019-11-14

申请人： SELUX DIAGNOSTICS, INC.

发明人： Kelly Flentie ,  Eric Stern

IPC分类号： C12Q1/20 ,  G16H20/10 ,  G16H10/40 ,  C12Q3/00

CPC分类号： C12Q1/20 ,  C12Q3/00 ,  G16H10/40 ,  G16H20/10

摘要： An improved system, method and interface for automated rapid antimicrobial susceptibility testing (AST) is disclosed which includes, in one aspect, a carrier population station comprising a workstation having a graphic user interface (GUI). The GUI accepts information from a lab technologist, including information related to a scope of testing to be performed on a patient sample. The GUI controls intelligent assignment of patient samples to test panels in a manner that maximize utilization of the test carrier by grouping together samples of similar tests scopes and advantageously testing those samples using one multiplexed test panel. Customizing workflow in accordance with test scope to facilitate parallel processing of multiple samples advantageously reduces laboratory waste, decreases test latencies, increases AST system throughput and efficiency, and thus lowers the costs to the AST lab.

公开(公告)号：US11691145B2

公开(公告)日：2023-07-04

申请号：US17496409

申请日：2021-10-07

申请人： Wisconsin Alumni Research Foundation

发明人： David J. Beebe ,  Duane S. Juang ,  Jiaquan Yu ,  Jo Handelsman

IPC分类号： B01L3/00 ,  C12Q1/20 ,  C40B60/12

CPC分类号： B01L3/502715 ,  C12Q1/20 ,  C40B60/12 ,  B01L2300/087 ,  B01L2300/0848 ,  B01L2300/123

摘要： A platform and method for conducting multi-variable combinational interactions are provided. An array of multiplexing chambers in formed in a body. The body also includes a common well communicating with each multiplexing chamber of the array of multiplexing chambers and a plurality of variable wells. Each of variable wells communicates with at least one multiplexing chamber of the array of multiplexing chambers. The common well is loaded with a first variable and different variables are loaded in each of the plurality of variable wells. The interaction of the first variable with at least one of the different variables in each multiplexing chamber of the array of multiplexing chambers is observed.

公开(公告)号：US20230167481A1

公开(公告)日：2023-06-01

申请号：US17922210

申请日：2021-05-04

申请人： UNIVERSITEIT ANTWERPEN ,  VRIJE UNIVERSITEIT BRUSSEL

发明人： Tatevik CHALYAN ,  Wendy MEULEBROECK ,  Kurt ROCHLITZ ,  Stijn VERWULGEN ,  Sander VAN GOETHEM ,  Chris BAELUS ,  Muriel DE BOECK ,  Herman GOOSSENS ,  Heidi OTTEVAERE ,  Hugo THIENPONT ,  Pieter MOONS

IPC分类号： C12Q1/20 ,  B01L3/00 ,  C12M3/06

CPC分类号： C12Q1/20 ,  B01L3/502715 ,  C12M23/16 ,  B01L2300/0681 ,  B01L2300/168 ,  B01L2200/16 ,  B01L2300/0861 ,  B01L2300/0654

摘要： The invention relates in a first aspect to an improved fluidic device for determining a property of a microbe. The device comprising a bottom layer comprising a plurality of light-transmissive wells; and a top layer comprising an injection opening and a fluid distribution system. Each of said plurality of distribution channels has: essentially the same channel length between the inlet end and the outlet end; and essentially the same channel volume. The invention relates in a second aspect to a use of the device for determining a susceptibility of a microbe, preferably a bacterium, to an antimicrobial drug.

公开(公告)号：US20220097058A1

公开(公告)日：2022-03-31

申请号：US17496409

申请日：2021-10-07

申请人： Wisconsin Alumni Research Foundation

发明人： David J. Beebe ,  Duane S. Juang ,  Jiaquan Yu ,  Jo Handelsman

IPC分类号： B01L3/00 ,  C12Q1/20 ,  C40B60/12

摘要： A platform and method for conducting multi-variable combinational interactions are provided. An array of multiplexing chambers in formed in a body. The body also includes a common well communicating with each multiplexing chamber of the array of multiplexing chambers and a plurality of variable wells. Each of variable wells communicates with at least one multiplexing chamber of the array of multiplexing chambers. The common well is loaded with a first variable and different variables are loaded in each of the plurality of variable wells. The interaction of the first variable with at least one of the different variables in each multiplexing chamber of the array of multiplexing chambers is observed.

公开(公告)号：US20210348207A1

公开(公告)日：2021-11-11

申请号：US17277857

申请日：2019-09-23

申请人： Emory University

发明人： David Weiss ,  Victor Band ,  David Hufnagel

IPC分类号： C12Q1/20 ,  A61K45/06

摘要： This disclosure contemplates detecting antimicrobial heteroresistance to guide antibiotic or other antimicrobial therapy. In certain embodiments, this disclosure contemplates avoiding a monotherapy with antibiotics to which an individual bacterial pie isolate is heteroresistant, because this can lead to treatment failure. Thus, in certain embodiments, one uses a combination of antibiotics to which a bacterium is individually heteroresistant.

公开(公告)号：US20210254126A1

公开(公告)日：2021-08-19

申请号：US17252189

申请日：2019-07-16

申请人： BACTEROMIC Sp. z o.o.

发明人： Pawel DEBSKI ,  Piotr GARSTECKI

IPC分类号： C12Q1/18 ,  C12Q1/20

摘要： Disclosed are methods and systems for rapidly testing antimicrobial susceptibility, wherein the qualitative and quantitative susceptibility of an inoculated microorganism against an antimicrobial agent or a combination of antimicrobial agents is determined as a function of one or more slopes of linear trends α of data of readouts.

公开(公告)号：US10831156B2

公开(公告)日：2020-11-10

申请号：US16095537

申请日：2017-04-27

申请人： BIOMERIEUX ,  BIOASTER

发明人： Alice Douet ,  Quentin Josso

IPC分类号： G03H1/08 ,  G03H1/04 ,  G01N15/14 ,  G06T7/55 ,  C12Q1/20 ,  G01N15/10 ,  G01N21/45 ,  G06T7/00

摘要： A device for acquisition of particles present in a sample includes a spatially coherent light source, an optical system, and an image sensor placed in the focal plane of the optical system. The image sensor is configured to capture an intensity image. A computational unit of the device is configured to construct a series of electromagnetic propagation matrices obtained for a plurality of defocusing offsets relative to a plane of focus of the optics. The computational unit is also configured to determine a first average focused electromagnetic matrix for the particles from the series of electromagnetic matrices, identifying at least one of the particles in the first electromagnetic matrix and storing the coordinates of said particle, and determining a second electromagnetic matrix at a distance of focus on a particle identified from the components of the series of electromagnetic matrices having the stored coordinates.

公开(公告)号：US09365886B2

公开(公告)日：2016-06-14

申请号：US14404849

申请日：2013-06-06

申请人： Silvio Brocco

发明人： Silvio Brocco

IPC分类号： C12Q1/20 ,  C12Q1/18 ,  C12M1/22 ,  C12M1/00

CPC分类号： C12Q1/20 ,  C12M23/10 ,  C12M23/34 ,  C12Q1/18

摘要： A device for microbiological analyzes is provided. More specifically, a device is provided for standardizing the crossing and allowing a perfect angle of 90° between two graduated paper strips impregnated with a predefined concentration gradient of an antimicrobial agent, for evaluating their synergistic effect on the minimum inhibitory concentration (MIC) on a bacterial culture medium.

摘要翻译： 提供微生物分析装置。 更具体地，提供了一种用于标准化交叉并且在浸渍有预定浓度梯度的抗微生物剂之间的两个分级纸条之间具有90°的完美角度的装置，以评估它们对最小抑制浓度（MIC）的协同作用 细菌培养基。

公开(公告)号：US09090929B2

公开(公告)日：2015-07-28

申请号：US12613834

申请日：2009-11-06

申请人： Stephen Dimmer ,  Dagmara Klein

发明人： Stephen Dimmer ,  Dagmara Klein

IPC分类号： C12N1/20 ,  C12Q1/20 ,  C12Q1/04

CPC分类号： C12Q1/045 ,  C12N1/20

摘要： A medium for the growth of vancomycin-resistant Enterococci comprising:— (i) a nutrient medium with an energy source effective to support growth and reproduction of vancomycin-resistant Enterococci; (ii) an effective amount of one or more selective agents to inhibit the growth of microorganisms other than vancomycin-resistant Enterococci; (iii) a Krebs cycle intermediate.

摘要翻译： 用于万古霉素耐药性肠球菌生长的培养基包括：（i）具有有效支持万古霉素耐药性肠球菌生长和繁殖的能量源的营养培养基; （ii）有效量的一种或多种选择剂以抑制非万古霉素肠球菌以外的微生物的生长; （iii）克雷布斯循环中间体。

公开(公告)号：US20150147775A1

公开(公告)日：2015-05-28

申请号：US14547487

申请日：2014-11-19

申请人： CORNING INCORPORATED

发明人： Derek Michael Fiacco ,  Jeffrey Glenn Lynn ,  Kaitlyn Mary Matias ,  Florence Christine Monique Verrier ,  Ying Wei

IPC分类号： C12Q1/20 ,  C12N1/20

CPC分类号： C12Q1/20 ,  C12N1/20 ,  G01N2333/21 ,  G01N2333/245 ,  G01N2333/265 ,  G01N2333/31

摘要： A method that includes the steps: inoculating nutrient agar with bacterial stock to form a culture; incubating the culture to form a first incubated culture; incubating a portion of the first culture with nutrient agar to form a second culture; incubating a portion of the second culture to form a third culture; incubating the third culture to form an inoculated test plate; forming an inoculum by suspending bacteria from the inoculated test plate in a buffered test solution, adjusting the pH to ˜7 to 8, and adding organic soil at a concentration of approximately 10% to 30% by weight; inoculating a silver-containing surface region of a test carrier with a portion of the inoculum; incubating the inoculated test carrier; washing the test carrier in a neutralizing solution to form a residual test inoculum; and calculating the percent reduction in the number of surviving bacterial colonies in the residual test inoculum.

摘要翻译： 一种包括以下步骤的方法：用细菌储存培养营养琼脂以形成培养物; 孵育培养物以形成第一培养培养物; 将一部分第一培养物与营养琼脂孵育以形成第二培养物; 孵育一部分第二培养物以形成第三培养物; 培养第三培养物以形成接种的测试板; 通过将接种的测试板上的细菌悬浮在缓冲的测试溶液中，将pH调节至约7至8并加入浓度为约10重量％至30重量％的有机土壤来形成接种物; 用一部分接种物接种试验载体的含银表面区域; 培养接种的试验载体; 在中和溶液中洗涤测试载体以形成残留测试接种物; 并计算残留测试接种物中存活的细菌菌落数量的百分比减少。