公开(公告)号：US08604426B2

公开(公告)日：2013-12-10

申请号：US13772701

申请日：2013-02-21

申请人： Bruker Daltonik GmbH

发明人： Ralf Hartmer

IPC分类号： H01J49/10 ,  G01N33/68 ,  B01D59/44

CPC分类号： G01N33/6848 ,  H01J49/0072

摘要： Radical anions for use in the fragmentation of positively charged biopolymer ions by means of electron transfer are produced from substances previously unknown for use as ETD production substances. The inventive substances produce radical anions that lead to electron transfer dissociations with a high yield of fragment ions. The substances have high volatility that allows them to be kept in unheated containers outside the vacuum system and transported into the vacuum system to an in vacuum electron attachment ion source via unheated lines and low molecular weights that allow the measurement of even very light fragment ions. In one embodiment, a suitable substance is 1-3-5-7-cyclooctatetraene.

摘要翻译： 用于通过电子转移使带正电荷的生物聚合物离子分解的自由基阴离子由以前未知的用作ETD生产物质的物质产生。 本发明的物质产生导致电子转移离解的自由基阴离子，具有高产率的碎片离子。 这些物质具有高挥发性，使其能够保持在真空系统外部的未加热的容器中，并通过未加热的管线和低分子量输送到真空电子附着离子源，允许测量甚至非常轻的碎片离子。 在一个实施方案中，合适的物质是1-3-5-7-环辛四烯。

公开(公告)号：US20130181123A1

公开(公告)日：2013-07-18

申请号：US13786001

申请日：2013-03-05

申请人： BRUKER DALTONIK GMBH

发明人： Oliver Rather

IPC分类号： H01J49/00

CPC分类号： H01J49/0036 ,  H01J49/0009 ,  H01J49/40

摘要： The invention relates to time-of-flight mass spectrometers in which individual time-of-flight spectra are measured by detection systems with limited dynamic measurement range and are summed to sum spectra. The invention proposes a method to increase the dynamic range of measurement of the spectrum. To achieve this, those ions signals whose measured values display saturation of the analog-to-digital converter (ADC) are replaced by correction values, particularly if several successive measured values are in saturation. The correction values are obtained from the width of the signals, preferably simply from the number of measured values in saturation.

摘要翻译： 本发明涉及飞行时间质谱仪，其中通过具有有限动态测量范围的检测系统测量单个飞行时间谱，并且将其与求和谱相加。 本发明提出了增加光谱测量动态范围的方法。 为了实现这一点，那些其测量值显示模数转换器（ADC）的饱和度的离子信号被校正值代替，特别是如果几个连续的测量值处于饱和状态。 校正值从信号的宽度获得，优选地简单地来自饱和度中的测量值的数量。

公开(公告)号：US20040094703A1

公开(公告)日：2004-05-20

申请号：US10298169

申请日：2002-11-15

申请人： Bruker Daltonik GMBH

发明人： Armin Holle

IPC分类号： H01J049/40

CPC分类号： H01J49/405

摘要： The invention relates to an energy-focusing and space-angle focusing reflector for time-of-flight mass spectrometers. The invention consists in producing an adjustable space-angle focusing system by means of an adjustably weaker field with curved equipotential lines at the end of the reflector instead of a fully homogeneous electrical reflection field.

摘要翻译： 本发明涉及一种用于飞行时间质谱仪的能量聚焦和空间角聚焦反射器。 本发明在于通过在反射器的末端具有弯曲的等电位线的可调节较弱的场来产生可调节的空间角度聚焦系统，而不是完全均匀的电反射场。

公开(公告)号：US20030136903A1

公开(公告)日：2003-07-24

申请号：US10316173

申请日：2002-12-10

申请人： Bruker Daltonik GMBH

发明人： Gerhard Weiss ,  Carsten Stoermer ,  Oliver Rather

IPC分类号： H01J049/40

CPC分类号： H01J49/401

摘要： The invention relates to time-of-flight mass spectrometers, equipped with ion reflector and ion detector, with orthogonal ion injection and outpulsing of a segment of the ion beam perpendicular to the direction of injection in a pulser. The invention is directed to a time-of-flight mass spectrometer in which a reflector and an ion detector each have an angular offset about an axis that is perpendicular to the respective directions of injection and deflection. This allows a large distance to be used between the pulser and detector with the highest possible utilization of ions.

摘要翻译： 本发明涉及配备有离子反射器和离子检测器的飞行时间质谱仪，其具有与脉冲发生器中的注射方向垂直的离子束的正交离子注入和脉冲。 本发明涉及一种飞行时间质谱仪，其中反射器和离子检测器各自具有围绕垂直于各个注射和偏转方向的轴线的角度偏移。 这允许在脉冲发生器和检测器之间使用较大的距离，以最大限度地利用离子。

公开(公告)号：US20030066802A1

公开(公告)日：2003-04-10

申请号：US10244826

申请日：2002-09-16

申请人： Bruker Daltonik GmbH

发明人： Bernd Jastorff ,  Reinhold Stormann ,  Andreas Germanus ,  Jochen Franzen

IPC分类号： B01D015/08

CPC分类号： G16C20/10 ,  G01N30/6095 ,  G01N30/7233 ,  G01N33/48714 ,  G16C20/20

摘要： The invention relates to the elucidation of the breakdown of foreign substance in the metabolism of a liquid, chemical or biological reaction system by the analytical determination of the breakdown products (metabolites) produced. It is common practice for this elucidation to be carried out nullblindnull, i.e. without systematic prediction of the breakdown substances, by comparison and subtraction of analytical data sets, which are obtained by, for example, separating the substances in the liquid using liquid chromatography and measuring them using mass spectrometry before and after the foreign substance has been added. Unlike the current method, the invention consists of first calculating a nullvirtualnull breakdown of the foreign substance, taking into account all the possible branches of the breakdown path according to a set of breakdown rules, which can be determined beforehand, so that the predicted potential breakdown products can be looked for selectively by using a more finally tuned method of measurement.

摘要翻译： 本发明涉及通过生产的分解产物（代谢物）的分析测定来阐明液体，化学或生物反应体系的代谢中外来物质的分解。 通过比较和减去分析数据集，通过例如使用液相色谱法分离液体中的物质而获得的这种解析数据集是通常的做法，即通过实施“盲目”，即没有系统地预测分解物质 并且在添加异物之前和之后使用质谱法测量它们。 本发明与现有方法不同，首先计算异物的“虚拟”故障，考虑到可以预先确定的一组故障规则，将故障路径的所有可能的分支考虑在内，从而预测 可以通过使用更加最终调谐的测量方法来选择性地寻找潜在的分解产物。

公开(公告)号：US11480579B2

公开(公告)日：2022-10-25

申请号：US16464288

申请日：2016-11-30

申请人： Bruker Daltonik GmbH

发明人： Karsten Becker ,  Evgeny Idelevich

IPC分类号： G01N33/68 ,  C12Q1/18

摘要： The invention relates to a method for the preparation of living, microbial samples and microorganisms for subsequent mass spectrometric measurement and evaluation. Findings which can be derived from such a measurement can particularly serve the faster identification of microorganisms in the microbial sample according to species/subspecies and/or the fast determination of resistance/sensitivity of the microorganisms to antimicrobial substances and/or the further characterization of microorganisms, for example in respect of pathogenicity, virulence and metabolism. According to a preferred embodiment of the invention, the preparation particularly takes place directly on a mass spectrometric sample support.

公开(公告)号：US11473122B2

公开(公告)日：2022-10-18

申请号：US14996919

申请日：2016-01-15

申请人： Bruker Daltonik GmbH

发明人： Thomas Maier

IPC分类号： C12Q1/24 ,  G01N33/68 ,  C12Q1/04

摘要： The invention mainly relates to the mass spectrometric identification of pathogens in blood cultures from bloodstream infections (septicemia). The invention provides a method with which microbial pathogens can be separated in purified form from blood after a relatively brief cultivation in a blood culture flask, without any interfering human proteins or any residual fractions of blood particles such as erythrocytes and leukocytes, and can be directly identified by mass spectrometric measurement of their protein profiles. The method is based on the use of relatively strong tensides to destroy the blood particles by dissolving the weak cell membranes and most of the internal structures of the blood particles; in spite of the fact that tensides are regarded as strong ionization inhibitors in MALDI and other ionization processes required for mass spectrometric measurements. This method allows unknown pathogens to be obtained in their pure form by centrifuging or filtration and to be identified on the taxonomic level of species or subspecies. Problems with DNA from high levels of leukocytes can be resolved by special measures. After sufficient cultivation, the identification in a mass spectrometric laboratory takes only half an hour.

公开(公告)号：US11348771B2

公开(公告)日：2022-05-31

申请号：US17095908

申请日：2020-11-12

申请人： Bruker Daltonik GmbH

发明人： Sören-Oliver Deininger ,  Martin Schürenberg

IPC分类号： H01J49/00 ,  H01J49/04 ,  G01N1/28 ,  H01J49/40 ,  H01J49/16

摘要： The invention relates to the identification and visualization of the spatial distribution of particular tissue states in histological tissue sections from mass spectrometric signals acquired with spatial resolution. The invention proposes a method by means of which regions of the tissue with similar mass spectra are found automatically, and it is further proposed that mass spectra of these regions are summed in order to improve the spectral quality to such an extent that known markers for tissue degenerations can be identified with increased certainty. Regions of similar mass spectra can be interconnected on a large scale, but can also be isolated from each other on a small scale.

公开(公告)号：US11226308B2

公开(公告)日：2022-01-18

申请号：US16589844

申请日：2019-10-01

申请人： Bruker Daltonik GmbH

发明人： Oliver Räther ,  Karsten Michelmann

IPC分类号： G01N27/622 ,  G16B40/10 ,  G06F17/18

摘要： The invention provides methods and devices for determining the reduced ion mobility Ko of an ion species by trapped ion mobility spectrometry wherein the reduced ion mobility Ko is determined from a measured arrival time tm of the ion species and predetermined instrumental parameters by applying the inverse of a system function: Ko=SYS−1(tm,pi) or from multiple arrival times tm,i of the ion species measured for multiple values of an instrumental parameter.

公开(公告)号：US11221338B2

公开(公告)日：2022-01-11

申请号：US16342088

申请日：2017-09-22

申请人： Bruker Daltonik GmbH

发明人： Tobias Boskamp

IPC分类号： G01N33/68 ,  G16B40/10 ,  H01J49/00

摘要： The invention relates to a method to evaluate mass spectrometry data for the analysis of peptides from biological samples, particularly MALDI-TOF mass spectrometry data, comprising the following steps: a) provide expected mass defects; b) determine measured mass defects, i.e. the mass defects resulting from the mass spectrometry data; c) compare the measured mass defects with the expected mass defects.