公开(公告)号：US11891664B2

公开(公告)日：2024-02-06

申请号：US16613298

申请日：2018-05-16

Applicant: Exosome Diagnostics, Inc. ,  The Brigham and Women's Hospital

Inventor： James Hurley ,  Christine Coticchia ,  Robert Kitchen ,  Vasisht Tadigotla ,  Johan Karl Olov Skog ,  Jamil Azzi

IPC: C12Q1/6883 ,  G01N1/28 ,  G01N33/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  G01N1/28 ,  G01N33/6893 ,  G01N2800/245 ,  G01N2800/347

Abstract: The invention relates generally to the use of microvesicle biomarkers such as nucleic acids, including nucleic acid signatures, and/or proteins for assessing a kidney transplant rejection in a patient. The invention further relates to assessing, and/or to monitoring kidney transplant rejection in patients who have received a renal transplant.

公开(公告)号：US20220412971A1

公开(公告)日：2022-12-29

申请号：US17761398

申请日：2020-09-18

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Olubode A. OGUNLUSI ,  Robert Raymond KITCHEN ,  Douglas ROBERTS ,  Wei YU

IPC: G01N33/543 ,  C12N15/10

Abstract: The present disclosure relates to compositions, methods and kits for the isolation of extracellular vesicles. The compositions, methods and kits can comprise a high-density liquid reagent that facilitates the sequestration of extracellular vesicles bound to magnetic beads.

公开(公告)号：US11345957B2

公开(公告)日：2022-05-31

申请号：US16631603

申请日：2018-07-18

Applicant: Exosome Diagnostics, Inc.

Inventor： Robert Kitchen ,  Michael Valentino ,  Johan Skog ,  Vasisht Tadigotla ,  Dalin Chan ,  Sudipto Chakrabortty ,  James Hurley

IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/6886

Abstract: The present disclosure relates to methods for treating glioblastoma in a subject in need thereof using gene signatures in exosomal RNA derived from the subject. The gene signatures comprise: at least one of FAM229B, ZNF35, CTD-2647L4.4, CABP5, CYP20A1, CEP126, DTX2P1-UPK3BP1-PMS2P11, RP11-507K12.1, KRBA2, CALD1, LRFN1, RP2, SLC2A13, CDKL3, SLC8A3, ANTXR2, TIGD5, AC074289.1 RP11-932O9.7; at least one of tRNA-Lys-CTT-2-2, tRNA-Pro-AGG-2-7, LAMTOR2, RAD51AP1, DENND2A, A1BG, THSD1, CSF1, RP11-332M2.1, ZNF717, ZNF860, ORC6, Clorf50, PSPH, HIST1H4C, CYP2U1, THAP8, TMEM192, NAA20; or at least one of CREBBP, CXCR2 and S100A9. The treatment methods comprise measuring the expression level of at least one of the aforementioned genes in exosomal RNA from a subject and administering to the subject a glioblastoma treatment based on the expression level(s).

公开(公告)号：US11268085B2

公开(公告)日：2022-03-08

申请号：US16595278

申请日：2019-10-07

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20200208213A1

公开(公告)日：2020-07-02

申请号：US16342572

申请日：2017-10-23

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan SKOG ,  Sudipto CHAKRABORTTY ,  Dalin CHAN ,  Michael VALENTINO ,  Vasisht TADIGOTLA ,  Robert KITCHEN ,  Dominik GRIMM ,  Wei YU

IPC: C12Q1/6874 ,  G01N1/28 ,  C12N15/10 ,  G01N33/543

Abstract: The invention provides a series of steps that prepare nucleic acids (RNA and/or DNA) isolated from extracellular vesicles for sequencing. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected. These can then be used to identify various attributes such as gene expression, alternative splicing, and the detection of both somatic and germline mutations including single nucleotide variants (SNV) and structural variations (insertions/deletions, fusions, inversions).

公开(公告)号：US20200149036A1

公开(公告)日：2020-05-14

申请号：US16473604

申请日：2018-01-02

Applicant: Exosome Diagnostics, Inc.

Inventor： Daniel ENDERLE ,  Johan SKOG ,  Phillipp TORKLER ,  Alexandra SPIEL ,  Mikkel NOERHOLM

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: The invention provides a series of steps to distinguish RNA from DNA in assays downstream of a combined isolation. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected.

公开(公告)号：US20200056244A1

公开(公告)日：2020-02-20

申请号：US16375237

申请日：2019-04-04

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Mikkel NOERHOLM

IPC: C12Q1/6886

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

公开(公告)号：US10465183B2

公开(公告)日：2019-11-05

申请号：US15325021

申请日：2015-07-09

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20170198280A1

公开(公告)日：2017-07-13

申请号：US15325021

申请日：2015-07-09

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Johan Karl Olov Skog ,  Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm

IPC: C12N15/10

Abstract: The invention provides novel methods and kits for isolating nucleic acids from biological samples, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, and for extracting nucleic acids from the microvesicles and/or from the biological samples.

公开(公告)号：US20150353920A1

公开(公告)日：2015-12-10

申请号：US14759238

申请日：2014-01-03

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： Daniel Enderle ,  Aparna Ramachandran ,  Haoheng Yan ,  Emily Berghoff ,  Tai-Fen Wei ,  Mikkel Noerholm ,  Johan Karl Olav Skog

IPC: C12N15/10

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

Abstract translation: 本发明提供从生物样品中分离微泡并从微泡中提取核酸的新方法。