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公开(公告)号：US09080973B2
公开(公告)日：2015-07-14
申请号：US12765695
申请日：2010-04-22
Applicant: Nicholas L. Abbott , I-Hsin Lin , Christopher J. Murphy , Jugal Gupta
Inventor： Nicholas L. Abbott , I-Hsin Lin , Christopher J. Murphy , Jugal Gupta
IPC: G01N21/77 , G01N21/85 , G01N21/88 , G01N31/22 , G01N33/52 , G01N21/51 , G01N21/21 , G01N21/64 , G01N21/47
CPC classification number: G01N33/92 , G01N21/21 , G01N21/49 , G01N21/51 , G01N21/6428 , G01N21/77 , G01N2021/4704 , G01N2021/4792 , G01N2021/6439 , G01N2400/50
Abstract: Devices and methods for using changes in the defects in micrometer sized dispersed liquid crystal domains to detect or quantify analytes in a test sample, including endotoxin lipopolysaccharide (LPS), are disclosed. The dispersed liquid crystal microdomains are exposed to the test sample, and any changes in the number of defects in the liquid crystal microdomains are detected by detecting changes in the anchoring configuration of the microdomains. Such changes in anchoring configuration indicate the presence of analyte in the test sample.
Abstract translation: 公开了使用微米级分散液晶畴中的缺陷变化来检测或定量测试样品（包括内毒素脂多糖（LPS））中的分析物的装置和方法。 将分散的液晶微区暴露于测试样品，并且通过检测微区域的锚定构型的变化来检测液晶微区中的缺陷数量的任何变化。 锚固构型的这种变化表明测试样品中分析物的存在。
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公开(公告)号：US08569043B2
公开(公告)日：2013-10-29
申请号：US12837471
申请日：2010-07-15
Applicant: Nicholas L. Abbott , Brian H. Clare , Paul J. Bertics
Inventor： Nicholas L. Abbott , Brian H. Clare , Paul J. Bertics
IPC: C12M1/34 , G01N33/553 , G01N33/547 , G01N33/53 , C07K1/10
CPC classification number: G01N33/542 , B01J19/0046 , B01J2219/00382 , B01J2219/00596 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01J2219/00743 , B01J2219/00745 , B01J2219/0075 , B82Y15/00 , B82Y30/00 , B82Y40/00 , C40B40/06 , C40B40/10 , C40B40/18 , C40B60/14 , G01N33/54373 , G01N33/6842
Abstract: A method for differentiating between a post-translationally modified peptide and a peptide contained in a sample, comprising: (a) contacting the sample with a peptide attachment surface to create a peptidized surface, wherein the sample includes at least one functional group; (b) contacting the peptidized surface with a recognition reagent that selectively binds or forms a complex with the post-translationally modified peptide in the sample to provide an incubated surface; and (c) contacting a liquid crystal with the incubated surface and detecting presence of post-translationally modified peptide in the sample with the liquid crystal.
Abstract translation: 一种用于区分样品中包含的翻译后修饰的肽和肽的方法，包括：（a）使样品与肽附着表面接触以产生肽化表面，其中所述样品包括至少一个官能团; （b）使肽化表面与识别试剂接触，所述识别试剂选择性地与样品中的翻译后修饰的肽结合或形成复合物以提供孵育的表面; 和（c）使液晶与孵育的表面接触并用液晶检测样品中翻译后修饰的肽的存在。
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公开(公告)号：US08329423B2
公开(公告)日：2012-12-11
申请号：US13011514
申请日：2011-01-21
Applicant: Nicholas L. Abbott , Joon-Seo Park , Sarah Teren , David J. Beebe , Eric A. Johnson
Inventor： Nicholas L. Abbott , Joon-Seo Park , Sarah Teren , David J. Beebe , Eric A. Johnson
IPC: G01N33/553
CPC classification number: C12Q1/37 , B82Y30/00 , G01N33/54373
Abstract: The present invention provides liquid crystal-based devices and methods for bioagent detection. In certain aspects, the present invention is directed to devices and methods utilizing liquid crystals and membranes containing polymerized targets that can report the presence of bioagents including, but not limited to, enzymes, antibodies, and toxins.
Abstract translation: 本发明提供了基于液晶的装置和用于生物鉴定检测的方法。 在某些方面，本发明涉及利用液晶和含有聚合靶的膜的装置和方法，所述聚合靶可以报告生物制剂的存在，包括但不限于酶，抗体和毒素。
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44.发明授权公开(公告)号：US08133680B2
公开(公告)日：2012-03-13
申请号：US10711517
申请日：2004-09-23
Applicant: Nicholas L. Abbott , Matthew L. Tingey , Brian H. Clare , Chang-Hyun Jang
Inventor： Nicholas L. Abbott , Matthew L. Tingey , Brian H. Clare , Chang-Hyun Jang
CPC classification number: B82Y30/00 , B01J19/0046 , B01J2219/00382 , B01J2219/00596 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01J2219/00743 , B01J2219/00745 , B01J2219/0075 , B82Y15/00 , C40B40/06 , C40B40/10 , C40B40/18 , C40B60/14 , G01N33/54373
Abstract: The present invention provides methods, devices and kits for detecting a ligand. The methods involve capturing a ligand from a sample with an affinity substrate that includes a receptor for a ligand, transferring captured ligand to a detection surface and detecting the ligand on the detection surface with a liquid crystal. Accordingly, the capture step is decoupled from the detection step.
Abstract translation: 本发明提供用于检测配体的方法，装置和试剂盒。 所述方法包括用具有配体受体的亲和底物从样品捕获配体，将捕获的配体转移到检测表面，并用液晶检测检测表面上的配体。 因此，捕获步骤与检测步骤分离。
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公开(公告)号：US08097457B2
公开(公告)日：2012-01-17
申请号：US13085846
申请日：2011-04-13
Applicant: Nicholas L. Abbott , Jeffrey C. Mohr , Christopher J. Murphy , Elise Huang , Juan J. DePablo , Nathan A. Lockwood , Sean P. Palecek
Inventor： Nicholas L. Abbott , Jeffrey C. Mohr , Christopher J. Murphy , Elise Huang , Juan J. DePablo , Nathan A. Lockwood , Sean P. Palecek
IPC: G01N33/53 , G01N33/567 , G01N33/566 , C12N5/00 , C12N5/02
CPC classification number: C12N5/0068 , C12N5/0606 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/90
Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.
Abstract translation: 本发明涉及可用于细胞培养的液晶底物及其使用方法。 在某些实施方案中，本发明提供了用于通过活细胞对细胞外基质组分的变化（例如重组）成像的方法和装置。
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Patent Agency Ranking
公开(公告)号：US20100233729A1
公开(公告)日：2010-09-16
申请号：US12786317
申请日：2010-05-24
Applicant: Nicholas L. Abbott , Jeffrey M. Brake
Inventor： Nicholas L. Abbott , Jeffrey M. Brake
IPC: G01N33/573 , G01N33/68 , G01N33/20
CPC classification number: C09K19/00 , C09K2019/528 , G01N33/54366 , Y10S436/805 , Y10T428/10 , Y10T428/1059 , Y10T428/1095
Abstract: A method of forming a liquid crystal device, includes: contacting an aqueous solution comprising a surfactant and a receptor molecule with a top surface of a liquid crystal. The liquid crystal is in a holding compartment of a substrate, and the receptor molecule is adsorbed on the top surface of the liquid crystal forming an interface between the liquid crystal and the aqueous solution. The receptor molecule is different than the surfactant. A method of detecting a compound in a flowing stream includes passing an aqueous solution over a top surface of a liquid crystal in a holding compartment of a substrate. The method also includes determining whether a change in the orientation of the liquid crystal occurs as the aqueous solution is passed over the top surface of the liquid crystal. A change in the orientation of the liquid crystal indicates the presence of the compound in the flowing stream.
Abstract translation: 形成液晶装置的方法包括：将包含表面活性剂和受体分子的水溶液与液晶的顶表面接触。 液晶位于基板的保持室中，受体分子被吸附在液晶的顶面上，形成液晶与水溶液的界面。 受体分子与表面活性剂不同。 在流动物流中检测化合物的方法包括将水溶液通过基板的保持室中的液晶的顶表面。 该方法还包括确定当水溶液通过液晶的顶表面时是否发生液晶的取向的变化。 液晶的取向的变化表示化合物在流动流中的存在。
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47.发明授权公开(公告)号：US07745220B2
公开(公告)日：2010-06-29
申请号：US11542432
申请日：2006-10-03
Applicant: Nicholas L. Abbott , Brian H. Clare
Inventor： Nicholas L. Abbott , Brian H. Clare
IPC: G01N31/00
CPC classification number: C09K19/12 , B82Y15/00 , B82Y30/00 , B82Y40/00 , C09K19/00 , G01N21/21 , G01N33/54373 , G01N2021/7769 , Y10T436/2575
Abstract: The present invention provides devices and methods for detection of analytes based on measuring the anchoring strength of liquid crystals having distorted geometries. Methods for detecting an analyte in a sample include the steps of: (a) capturing an analyte on a substrate surface wherein the substrate surface defines an easy axis when in contact with a liquid crystal. Substrate surface and liquid crystal are brought into contact and an analyte-dependent departure in the orientation of the liquid crystal from the easy axis of the substrate surface is measured. This departure indicates the presence of the analyte in the sample.
Abstract translation: 本发明提供了基于测量具有变形几何形状的液晶的锚定强度来检测分析物的装置和方法。 用于检测样品中的分析物的方法包括以下步骤：（a）捕获基底表面上的分析物，其中当与液晶接触时，所述基底表面限定易轴。 使基板表面和液晶接触，并且测量液晶从基板表面的容易轴的取向的分析物依赖性偏离。 该离开表示样品中分析物的存在。
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公开(公告)号：US07724319B2
公开(公告)日：2010-05-25
申请号：US12324403
申请日：2008-11-26
Applicant: Nicholas L. Abbott , Jeffrey M. Brake
Inventor： Nicholas L. Abbott , Jeffrey M. Brake
IPC: G02F1/13 , G02F1/1333 , G01N33/53 , G01N33/566 , G01N21/21 , G01N21/75 , G01N21/85
CPC classification number: C09K19/00 , C09K2019/528 , G01N33/54366 , Y10S436/805 , Y10T428/10 , Y10T428/1059 , Y10T428/1095
Abstract: A method of forming a liquid crystal device, includes: contacting an aqueous solution comprising a surfactant and a receptor molecule with a top surface of a liquid crystal. The liquid crystal is in a holding compartment of a substrate, and the receptor molecule is adsorbed on the top surface of the liquid crystal forming an interface between the liquid crystal and the aqueous solution. The receptor molecule is different than the surfactant. A method of detecting a compound in a flowing stream includes passing an aqueous solution over a top surface of a liquid crystal in a holding compartment of a substrate. The method also includes determining whether a change in the orientation of the liquid crystal occurs as the aqueous solution is passed over the top surface of the liquid crystal. A change in the orientation of the liquid crystal indicates the presence of the compound in the flowing stream.
Abstract translation: 形成液晶装置的方法包括：将包含表面活性剂和受体分子的水溶液与液晶的顶表面接触。 液晶位于基板的保持室中，受体分子被吸附在液晶的顶面上，形成液晶与水溶液的界面。 受体分子与表面活性剂不同。 在流动物流中检测化合物的方法包括将水溶液通过基板的保持室中的液晶的顶表面。 该方法还包括确定当水溶液通过液晶的顶表面时是否发生液晶的取向的变化。 液晶的取向的变化表示化合物在流动流中的存在。
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公开(公告)号：US07459124B2
公开(公告)日：2008-12-02
申请号：US11523319
申请日：2006-09-19
Applicant: Nicholas L. Abbott , Jeffrey M. Brake
Inventor： Nicholas L. Abbott , Jeffrey M. Brake
CPC classification number: C09K19/00 , C09K2019/528 , G01N33/54366 , Y10S436/805 , Y10T428/10 , Y10T428/1059 , Y10T428/1095
Abstract: A method of forming a liquid crystal device, includes: contacting an aqueous solution comprising a surfactant and a receptor molecule with a top surface of a liquid crystal. The liquid crystal is in a holding compartment of a substrate, and the receptor molecule is adsorbed on the top surface of the liquid crystal forming an interface between the liquid crystal and the aqueous solution. The receptor molecule is different than the surfactant. A method of detecting a compound in a flowing stream includes passing an aqueous solution over a top surface of a liquid crystal in a holding compartment of a substrate. The method also includes determining whether a change in the orientation of the liquid crystal occurs as the aqueous solution is passed over the top surface of the liquid crystal. A change in the orientation of the liquid crystal indicates the presence of the compound in the flowing stream.
Abstract translation: 形成液晶装置的方法包括：将包含表面活性剂和受体分子的水溶液与液晶的顶表面接触。 液晶位于基板的保持室中，受体分子被吸附在液晶的顶面上，形成液晶与水溶液的界面。 受体分子与表面活性剂不同。 在流动物流中检测化合物的方法包括将水溶液通过基板的保持室中的液晶的顶表面。 该方法还包括确定当水溶液通过液晶的顶表面时是否发生液晶的取向的变化。 液晶的取向的变化表示化合物在流动流中的存在。
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公开(公告)号：US07303694B2
公开(公告)日：2007-12-04
申请号：US10892827
申请日：2004-07-16
Applicant: Christopher John Murphy , Nicholas L. Abbott , Yan-Yeung Luk , Sean Francis Campbell , Li-Lin Cheng , Chang-Hyun Jang
Inventor： Christopher John Murphy , Nicholas L. Abbott , Yan-Yeung Luk , Sean Francis Campbell , Li-Lin Cheng , Chang-Hyun Jang
CPC classification number: C09K19/42 , B82Y15/00 , B82Y30/00 , C07D311/24 , C09K19/3003 , C09K2019/0407 , C09K2019/301
Abstract: Liquid crystal compositions that exhibit little or no toxicity with respect to cells include liquid crystals with chemical functional groups such as fluorine atoms, fluorophenyl groups, or difluorophenyl groups. Liquid crystals with little or no toxicity to cell lines may be added to cell culture media or added to components used in cell culture media. Cells may be grown in cell culture media that includes liquid crystals that exhibit little or no toxicity to cells.
Abstract translation: 对细胞显示出很少或没有毒性的液晶组合物包括具有化学官能团如氟原子，氟苯基或二氟苯基的液晶。 可以向细胞培养基中加入对细胞系很少或没有毒性的液晶，或添加到细胞培养基中使用的组分。 细胞可以在细胞培养基中生长，其包括对细胞表现出很少或没有毒性的液晶。
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