公开(公告)号：US20090311749A1

公开(公告)日：2009-12-17

申请号：US12521856

申请日：2008-01-25

申请人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

发明人： Shinobu Takagi ,  Noriko Tsutsumi ,  Yuji Terui ,  XiangYu Kong

IPC分类号： C12P21/00

CPC分类号： C12P21/02 ,  C07K14/39 ,  C12N15/815

摘要： The present invention relates to a method for producing a polypeptide in a methylotrophic yeast host cell, wherein expression of the polypeptide is controlled by a methanol inducible promoter, comprising: i) expression of a positive regulator from a non-native promoter, said positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.

摘要翻译： 本发明涉及在甲基营养酵母宿主细胞中产生多肽的方法，其中多肽的表达由甲醇诱导型启动子控制，其包括：i）从非天然启动子，所述阳性调节子 激活甲醇诱导型启动子的转录，以及ii）不添加甲醇。

公开(公告)号：US07226773B2

公开(公告)日：2007-06-05

申请号：US10965499

申请日：2004-10-14

申请人： Martin Schulein, deceased ,  Torben Henriksen, legal representative ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

发明人： Martin Schulein, deceased ,  Torben Henriksen, legal representative ,  Lene Nonboe Andersen ,  Soren Flensted Lassen ,  Markus Sakari Kauppinen ,  Lene Lange ,  Ruby Ilum Nielsen ,  Shinobu Takagi ,  Michiko Ihara

IPC分类号： C12N9/42 ,  D06M16/00 ,  C07G17/00 ,  C11D3/386 ,  C07H21/04

CPC分类号： C12N9/2437 ,  A61K38/00 ,  C05F11/08 ,  C05F17/0036 ,  C07K2319/00 ,  C11D3/386 ,  C12P19/14 ,  C12Y302/01004 ,  D06M16/003 ,  D06P5/02 ,  D06P5/158 ,  D21C5/005 ,  D21H17/005 ,  D21H21/10 ,  Y02P20/145 ,  Y02W30/43

摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

公开(公告)号：US20060159971A1

公开(公告)日：2006-07-20

申请号：US10525234

申请日：2003-08-19

申请人： Shinobu Takagi ,  Masaki Shinkawa ,  Mikio Ura ,  Shinichi Yagi ,  Yasushi Kaneta ,  Tatsuo Hisada

发明人： Shinobu Takagi ,  Masaki Shinkawa ,  Mikio Ura ,  Shinichi Yagi ,  Yasushi Kaneta ,  Tatsuo Hisada

IPC分类号： H01M8/02 ,  B05D5/12 ,  B32B15/01 ,  H01M8/10 ,  C22C38/42

CPC分类号： H01M8/0228 ,  H01M8/0206 ,  H01M8/021 ,  H01M8/0254 ,  H01M2008/1095 ,  Y02P70/56 ,  Y10T428/12875 ,  Y10T428/12889 ,  Y10T428/12896 ,  Y10T428/12944 ,  Y10T428/12951 ,  Y10T428/12993

摘要： An Au plated film 12 is formed on the surface of a plate-formed metal base 13 composed of a metal less noble than Au, and the product is cut along a planned cutting line 18 reflecting a contour of a desired component, to thereby form a separator 10. Thus-formed separator 10 has the Au plated film 12 formed on the main surface 10a thereof, and has a cutting plane 16 formed as an end face 16 stretched up to the main surface 10a. The metal base 13 exposes in a part of the cutting plane 16, in a width of the exposed region of 1 mm or less. This is successful in providing a metal component for fuel cell which is satisfactory in the corrosion resistance and allows easy fabrication at low costs, a method of manufacturing the same, and also in providing a fuel cell having thus-fabricated metal component for fuel cell.

摘要翻译： 在由金属低于金属的金属构成的板状金属基体13的表面上形成Au镀膜12，并且沿着反映期望成分的轮廓的规划切割线18切割产品，从而形成 分离器10。 如此形成的隔板10具有形成在其主表面10a上的Au镀膜12，并且具有形成为延伸到主表面10a的端面16的切割平面16。 金属基底13在切割平面16的一部分中暴露在1mm或更小的暴露区域的宽度上。 这是成功地提供了用于燃料电池的金属部件，其耐腐蚀性良好并且容易以低成本制造，其制造方法以及提供具有如此制造的用于燃料电池的金属组分的燃料电池。

公开(公告)号：US20050100758A1

公开(公告)日：2005-05-12

申请号：US10981708

申请日：2004-11-05

申请人： Takeo Hisada ,  Yasushi Kaneta ,  Shinobu Takagi ,  Naoki Fuse

发明人： Takeo Hisada ,  Yasushi Kaneta ,  Shinobu Takagi ,  Naoki Fuse

IPC分类号： C25D3/48 ,  H01M8/02 ,  B32B15/01

CPC分类号： C25D3/48 ,  H01M8/0206 ,  H01M8/021 ,  H01M8/0228 ,  Y10T428/12889 ,  Y10T428/12979

摘要： Disclosed is a corrosion resistant conductive part, which is made by forming a thin plated gold layer of thickness 100 nm or less on a stainless steel sheet, and for which high corrosion resistance is guaranteed even with a very thin gold layer. The part is characterized in that the maximum value “v” of atomic concentration of gold given by Auger analysis is at least 98% of the total atomic concentrations of all the elements, which is deemed to be 100%, in the thin gold layer ranging from the surface to the interface of the gold layer and the metal substrate, at which the descending curve of gold crosses the ascending curve of the most predominant component of the substrate metal.

摘要翻译： 公开了一种耐腐蚀性导电部件，其通过在不锈钢板上形成厚度为100nm以下的薄镀金层而形成，并且即使使用非常薄的金层也能保证高耐腐蚀性。 该部分的特征在于，通过俄歇分析给出的金的原子浓度的最大值“v”是所有元素的总原子浓度的至少98％，其被认为是100％，在薄金层中 从金层和金属基底的表面到界面处，金的下降曲线与基底金属最主要成分的上升曲线交叉。

公开(公告)号：US06699593B2

公开(公告)日：2004-03-02

申请号：US10084434

申请日：2002-02-28

申请人： Yasushi Kaneta ,  Shinobu Takagi ,  Hiroaki Yoshida ,  Yoshihisa Suzuki ,  Masaki Shinkawa

发明人： Yasushi Kaneta ,  Shinobu Takagi ,  Hiroaki Yoshida ,  Yoshihisa Suzuki ,  Masaki Shinkawa

IPC分类号： B32B1501

CPC分类号： H01M8/0206 ,  B32B15/018 ,  H01M8/0202 ,  H01M8/021 ,  H01M8/0228 ,  H01M8/0254 ,  Y02P70/56 ,  Y10S428/925 ,  Y10S428/935 ,  Y10S428/938 ,  Y10T428/12875 ,  Y10T428/12889 ,  Y10T428/12896

摘要： A highly corrosion-resistant material and a corrosion-resistant member which are improved in corrosion resistance, adhesion, contact electrical resistance, electrical conductivity, airtightness, etc. and are suitable for use as, e.g., a metallic separator for polymer electrolyte fuel cells (PEFC); and a process for producing them. A thin noble-metal layer is formed on the desired part of the surface of a metallic base and then subjected to compression working. The coated base may further be subjected to anticorrosive treatment with a liquid phase containing a peroxide or ozone or with an active gas atmosphere.

摘要翻译： 耐腐蚀性，粘合性，接触电阻，导电性，气密性等改善的耐腐蚀性高的材料和耐腐蚀性构件，适用于例如聚合物电解质燃料电池用金属隔板（ PEFC）; 以及生产它们的过程。 在金属基材的表面的所需部分上形成薄的贵金属层，然后进行压缩加工。 涂覆的基底还可以用含有过氧化物或臭氧或活性气体气氛的液相进行防腐蚀处理。