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    Switchable Nucleic Acid Aptamer Probe and Uses Thereof in Tumor Living Cell Detection and in Vivo Detection
    41.
    发明申请
    Switchable Nucleic Acid Aptamer Probe and Uses Thereof in Tumor Living Cell Detection and in Vivo Detection 有权
    可切换的核酸适体探针及其在肿瘤生物细胞检测和体内检测中的应用

    公开(公告)号：US20130251638A1

    公开(公告)日：2013-09-26

    申请号：US13639843

    申请日：2011-03-22

    Applicant: Kemin Wang ,  Hui Shi ,  Xiaoxiao He ,  Xiaosheng Ye ,  Xu Wu ,  Qiuping Guo ,  Bing Zhou

    Inventor： Kemin Wang ,  Hui Shi ,  Xiaoxiao He ,  Xiaosheng Ye ,  Xu Wu ,  Qiuping Guo ,  Bing Zhou

    IPC: A61K49/00 ,  C12Q1/68

    CPC classification number: A61K48/0058 ,  A61K49/0054 ,  C12Q1/6818 ,  C12Q1/6886 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6432 ,  C12Q2525/301 ,  C12Q2525/205

    Abstract: The present invention relates to nucleic acid-based detection field, in particularly to a switchable nucleic acid aptamer probe and use thereof. The switchable nucleic acid aptamer probe includes a probe main body as well as a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment partially complemented with the nucleic acid aptamer fragment. The nucleic acid aptamer fragment and the nucleic acid fragment are linked by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and in vivo detection of tumor in living body. The probe of the invention holds relatively high stability, specificity and sensitivity, and its use is easy to operation, fast, sensitive, specific and of low cost.

    Abstract translation: 本发明涉及基于核酸的检测领域，特别涉及可切换的核酸适体探针及其用途。 可切换核酸适体探针包括分别连接到探针主体的两端的探针主体以及荧光发生单元和荧光淬灭单元。 探针主体包括具有特异性识别靶肿瘤细胞的功能的核酸适体片段和与核酸适体片段部分补充的核酸片段。 核酸适体片段和核酸片段通过长度为7〜15nm的连接片段连接，形成发夹结构。 核酸片段与核酸适体片段竞争性杂交的能力弱于目标肿瘤细胞。 使用本发明的探针可以是缓冲溶液中肿瘤活细胞的特异性检测，血清中肿瘤活细胞的有效检测以及生物体内肿瘤的实时荧光成像和体内检测中的至少一种。 本发明的探针具有较高的稳定性，特异性和灵敏度，其使用易于操作，快速，灵敏，特异性和低成本。

    PHASE SEPARATION DETECTION IN DOWNHOLE FLUID SAMPLING
    45.
    发明申请
    PHASE SEPARATION DETECTION IN DOWNHOLE FLUID SAMPLING 有权
    井下液体采样中的相分离检测

    公开(公告)号：US20100192684A1

    公开(公告)日：2010-08-05

    申请号：US12363835

    申请日：2009-02-02

    Applicant: Xu Wu ,  Tsutomu Yamate ,  Toru Terabayashi ,  Ricardo Vasques ,  Chengli Dong ,  Peter S. Hegeman

    Inventor： Xu Wu ,  Tsutomu Yamate ,  Toru Terabayashi ,  Ricardo Vasques ,  Chengli Dong ,  Peter S. Hegeman

    IPC: E21B49/08

    CPC classification number: E21B49/082

    Abstract: Example methods and apparatus to detect phase separation in downhole fluid sampling operations are disclosed. An example method to detect a phase separation condition of a fluid from a subterranean involves obtaining a sample of the fluid, measuring a first characteristic value of the sample, measuring a second characteristic value of the sample and comparing the first characteristic value to a first reference value associated with a single-phase condition of the fluid to generate a corresponding first comparison result. The example method then compares the second characteristic value to a second reference value associated with the single-phase condition of the fluid to generate a corresponding second comparison result and detects the phase separation condition of the fluid based on the first and second comparison results.

    Abstract translation: 公开了用于检测井下液体取样操作中的相分离的示例性方法和装置。 用于检测来自地下的流体的相分离条件的示例性方法包括获得流体样品，测量样品的第一特征值，测量样品的第二特征值，并将第一特征值与第一参考值进行比较 值与流体的单相条件相关联以产生相应的第一比较结果。 该示例方法然后将第二特征值与与流体的单相条件相关联的第二参考值进行比较，以产生相应的第二比较结果，并基于第一和第二比较结果检测流体的相分离条件。

    Compositions and methods for inducing osteogenesis
    48.
    发明授权
    Compositions and methods for inducing osteogenesis 失效
    诱导成骨的组合物和方法

    公开(公告)号：US07273864B2

    公开(公告)日：2007-09-25

    申请号：US10687220

    申请日：2003-10-15

    Applicant: Xu Wu ,  Sheng Ding ,  Nathanael S. Gray

    Inventor： Xu Wu ,  Sheng Ding ,  Nathanael S. Gray

    IPC: C07D473/40 ,  C07D473/34 ,  C07D473/18 ,  A61K31/522 ,  A61K31/52

    CPC classification number: C07D473/16 ,  C07D473/18 ,  C07D473/34 ,  C07D473/40

    Abstract: The present invention provides compositions and methods for differentiating and transdifferentiating mammalian cells into cells of an osteoblast lineage, using compounds of the following formula: wherein R4 is a functional group including, for example, C1-4alkyl, C3-8cycloalkyl, hydroxyl-C1-4alkyl, aryl-C0-3alkyl, substituted with 0-2 R4a groups, and heterocyclo-C0-2alkyl, optionally substituted with C1-4alkyl; R5 is hydrogen and R6 is a functional group including, for example, halogen, C1-4alkyl, —C(O)—C1-4alkyl, —SO2—N(R2b R2b), halo—C1-4alkyl, —O-aryl and —N(R7 R8), or when R5 and R6 are on adjacent ring atoms they are optionally taken together to form —O—(CH2)1-2—O—; and all pharmaceutically acceptable salts and hydrates thereof.

    Abstract translation: 本发明提供了使用下式化合物将哺乳动物细胞分化和转分化成成骨细胞谱系细胞的组合物和方法：其中R 4是包括例如C 1 -C 6烷基的官能团， 1-4个碳原子的烷基，C 3-8环烷基，羟基-C 1-4烷基，芳基-C 0-3-3烷基， 被0-2个R 4a取代的烷基和任选被C 1-4烷基取代的杂环-C 0-2-2烷基; R 5是氢，R 6是官能团，包括例如卤素，C 1-4烷基，-C（O） -C 1-4烷基，-SO 2 -N（R 2b，R 2b），卤代-C 1-4芳烷基，-O-芳基和-N（R 7 R 8），或当R 5 O >和R 6'在相邻的环原子上，它们任选地一起形成-O-（CH 2）2 - N 2 -O- ; 及其所有药学上可接受的盐和水合物。

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