利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

51 条记录 (耗时 0.027 秒)

    Switchable nucleic acid aptamer probe and uses thereof in tumor living cell detection and in vivo detection
    1.
    发明授权
    Switchable nucleic acid aptamer probe and uses thereof in tumor living cell detection and in vivo detection 有权
    可切换的核酸适体探针及其在肿瘤活细胞检测和体内检测中的应用

    公开(公告)号：US08883994B2

    公开(公告)日：2014-11-11

    申请号：US13639843

    申请日：2011-03-22

    申请人： Kemin Wang ,  Hui Shi ,  Xiaoxiao He ,  Xiaosheng Ye ,  Xu Wu ,  Qiuping Guo ,  Bing Zhou

    发明人： Kemin Wang ,  Hui Shi ,  Xiaoxiao He ,  Xiaosheng Ye ,  Xu Wu ,  Qiuping Guo ,  Bing Zhou

    IPC分类号： C07H21/02 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  C07H21/00 ,  G01N21/64

    CPC分类号： A61K48/0058 ,  A61K49/0054 ,  C12Q1/6818 ,  C12Q1/6886 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2021/6432 ,  C12Q2525/301 ,  C12Q2525/205

    摘要： A switch mode nucleic acid aptamer probe includes a probe main body, a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment linked to the nucleic acid aptamer fragment by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and intravital detection of tumor in living body.

    摘要翻译： 开关模式核酸适配体探针包括分别连接到探针主体的两端的探针主体，荧光发生单元和荧光淬灭单元。 探针主体包括具有特异性识别靶肿瘤细胞的功能的核酸适体片段和通过长度为7〜15nm的连接片段与核酸适体片段连接的核酸片段，以形成发夹结构 。 核酸片段与核酸适体片段竞争性杂交的能力弱于目标肿瘤细胞。 使用本发明的探针可以是缓冲溶液中肿瘤活细胞的特异性检测中的至少一种，血清中有效检测肿瘤活细胞，实时荧光成像和活体内肿瘤的活体检测。

    Phase separation detection in downhole fluid sampling
    7.
    发明授权
    Phase separation detection in downhole fluid sampling 有权
    井下液相采样中的相分离检测

    公开(公告)号：US08528396B2

    公开(公告)日：2013-09-10

    申请号：US12363835

    申请日：2009-02-02

    申请人： Xu Wu ,  Tsutomu Yamate ,  Toru Terabayashi ,  Ricardo Vasques ,  Chengli Dong ,  Peter S. Hegeman

    发明人： Xu Wu ,  Tsutomu Yamate ,  Toru Terabayashi ,  Ricardo Vasques ,  Chengli Dong ,  Peter S. Hegeman

    IPC分类号： E21B49/08

    CPC分类号： E21B49/082

    摘要： Example methods and apparatus to detect phase separation in downhole fluid sampling operations are disclosed. An example method to detect a phase separation condition of a fluid from a subterranean involves obtaining a sample of the fluid, measuring a first characteristic value of the sample, measuring a second characteristic value of the sample and comparing the first characteristic value to a first reference value associated with a single-phase condition of the fluid to generate a corresponding first comparison result. The example method then compares the second characteristic value to a second reference value associated with the single-phase condition of the fluid to generate a corresponding second comparison result and detects the phase separation condition of the fluid based on the first and second comparison results.

    摘要翻译： 公开了用于检测井下液体取样操作中的相分离的示例性方法和装置。 用于检测来自地下的流体的相分离条件的示例性方法包括获得流体样品，测量样品的第一特征值，测量样品的第二特征值，并将第一特征值与第一参考值进行比较 值与流体的单相条件相关联以产生相应的第一比较结果。 该示例方法然后将第二特征值与与流体的单相条件相关联的第二参考值进行比较，以产生相应的第二比较结果，并基于第一和第二比较结果检测流体的相分离条件。

    Pressure measuring device and method
    10.
    发明授权
    Pressure measuring device and method 有权
    压力测量装置及方法

    公开(公告)号：US07832276B2

    公开(公告)日：2010-11-16

    申请号：US12334584

    申请日：2008-12-15

    申请人： Xu Wu ,  Arthur Hartog ,  Dimitri Cadere ,  Tsutomu Yamate

    发明人： Xu Wu ,  Arthur Hartog ,  Dimitri Cadere ,  Tsutomu Yamate

    IPC分类号： G01F9/00

    CPC分类号： G01L9/0079 ,  G01L9/0077

    摘要： A device to measure a fluid pressure comprises a pressure sensing element 10 and a pressure readout element 20. The pressure sensing element 10 comprises a cavity 11 capped by a flexible membrane 13, the cavity having a length d that varies with the fluid pressure P1 applied on the flexible membrane 13. The pressure readout element 20 comprises a light source 24 for providing an incident beam of a determined wavelength range directed towards the cavity and an optical spectral analyzer 25 for measuring a power spectrum of a return beam reflected by the cavity, and processing means 27 for determining the cavity length d and the fluid pressure P1 based on the power spectrum.

    摘要翻译： 用于测量流体压力的装置包括压力感测元件10和压力读数元件20.压力感测元件10包括由柔性膜13封盖的空腔11，空腔具有随施加的流体压力P1而变化的长度d 压力读出元件20包括用于提供朝向空腔的确定的波长范围的入射光束的光源24和用于测量由空腔反射的返回光束的功率谱的光谱分析器25， 以及用于基于功率谱确定腔长度d和流体压力P1的处理装置27。