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公开(公告)号：US20110059204A1
公开(公告)日：2011-03-10
申请号：US12641929
申请日：2009-12-18
Applicant: Ethel Noland Jackson , David Richard Short , Dongming Xie , Zhixiong Xue , Quinn Qun Zhu
Inventor： Ethel Noland Jackson , David Richard Short , Dongming Xie , Zhixiong Xue , Quinn Qun Zhu
CPC classification number: C12P7/6427
Abstract: Methods for the production of omega-3 and/or omega-6 fatty acids in oleaginous yeasts grown on a fermentable carbon source selected from the group consisting of invert sucrose, glucose, fructose and combinations of these, provided that glucose is used in combination with invert sucrose and/or fructose. Specifically, methods are provided for production of linoleic acid, eicosadienoic acid, gamma-linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, n-6 docosapentaenoic acid, α-linolenic acid, stearidonic acid, eicosatrienoic acid, eicosatetraenoic acid, eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid.
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公开(公告)号：US07879591B2
公开(公告)日：2011-02-01
申请号：US12485141
申请日：2009-06-16
Applicant: Howard Glenn Damude , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
Inventor： Howard Glenn Damude , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
CPC classification number: C12P7/6472 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , A23V2250/187 , A23V2200/30
Abstract: Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
Abstract translation: 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便能够在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加和降低转化效率。
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43.发明授权Method for the production of resveratrol in a recombinant oleaginous microorganism 有权在重组油性微生物中生产白藜芦醇的方法公开(公告)号：US07772444B2
公开(公告)日：2010-08-10
申请号：US11436182
申请日：2006-05-17
Applicant: Lixuan Lisa Huang , Zhixiong Xue , Quinn Qun Zhu
Inventor： Lixuan Lisa Huang , Zhixiong Xue , Quinn Qun Zhu
IPC: A61K31/7034 , A61K31/05 , C07C39/04 , C07H15/203
CPC classification number: C12P7/22 , C12N9/1029 , C12N9/88 , C12N9/93 , C12N15/815
Abstract: Methods to produce resveratrol and/or resveratrol glucoside in a recombinant oleaginous microorganism are provided. Expression of a resveratrol synthase gene in combination with genes involved in the phenylpropanoid pathway enabled recombinant microbial production of resveratrol in significant amounts.
Abstract translation: 提供了在重组含油微生物中产生白藜芦醇和/或白藜芦醇葡糖苷的方法。 白藜芦醇合酶基因与参与苯丙素途径的基因组合的表达使得白藜芦醇的重组微生物产生显着。
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44.发明申请公开(公告)号：US20100175148A1
公开(公告)日：2010-07-08
申请号：US12725482
申请日：2010-03-17
Applicant: Howard Glenn Damude , Brian McGonigle , Quinn Qun Zhu , Zhixiong Xue
Inventor： Howard Glenn Damude , Brian McGonigle , Quinn Qun Zhu , Zhixiong Xue
IPC: C12P7/64 , C07H21/04 , C12N9/02 , C12N15/82 , C12N5/10 , C12N15/63 , A01H1/00 , A01H5/10 , A01H5/00
CPC classification number: C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.
Abstract translation: 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。
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45.发明申请公开(公告)号：US20100075387A1
公开(公告)日：2010-03-25
申请号：US12619706
申请日：2009-11-17
Applicant: HOWARD GLENN DAMUDE , BRIAN MCGONIGLE , ZHIXIONG XUE , QUINN QUN ZHU
Inventor： HOWARD GLENN DAMUDE , BRIAN MCGONIGLE , ZHIXIONG XUE , QUINN QUN ZHU
CPC classification number: C12N15/8247 , C11B1/025 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472
Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.
Abstract translation: 本发明涉及具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]的能力的“Dgr”9延伸酶。 公开了分离的核酸片段和重组构建体，其包含编码'Dgr 9延长酶的这种片段以及使用这些植物和油性酵母中的这些Dgr 9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。
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Patent Agency Ranking
公开(公告)号：US20090325265A1
公开(公告)日：2009-12-31
申请号：US12485141
申请日：2009-06-16
Applicant: Howard Glenn Damude , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
Inventor： Howard Glenn Damude , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
CPC classification number: C12P7/6472 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , A23V2250/187 , A23V2200/30
Abstract: Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.
Abstract translation: 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加Delta4去饱和转化效率。
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公开(公告)号：US20090311380A1
公开(公告)日：2009-12-17
申请号：US12466072
申请日：2009-05-14
Applicant: Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
Inventor： Howard Glenn Damude , Peter John Gillies , Daniel Joseph Macool , Stephen K. Picataggio , James John Ragghianti , John E. Seip , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC classification number: C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
Abstract translation: 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。
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48.发明授权公开(公告)号：US07556949B2
公开(公告)日：2009-07-07
申请号：US11779915
申请日：2007-07-19
Applicant: Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
Inventor： Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
CPC classification number: C12P7/6427 , C12N9/0083 , C12P7/6472
Abstract: The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.
Abstract translation: 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物以及使用这些Delta17去饱和酶在含油酵母中制备长链多不饱和脂肪酸（PUFA）的方法。
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49.发明授权Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效高山被孢霉溶血磷脂酸酰基转移酶同源物，用于改变多不饱和脂肪酸和含油生物中的油含量公开(公告)号：US07524658B2
公开(公告)日：2009-04-28
申请号：US11717800
申请日：2007-03-12
Applicant: Howard G. Damude , Zhixiong Xue
Inventor： Howard G. Damude , Zhixiong Xue
IPC: C12P7/64
CPC classification number: C12P7/6472 , C12N9/1029 , C12P7/6463
Abstract: Lysophosphatidic acid acyltransferase (LPAAT) participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment (identified as “LPAAT2”) isolated from Mortierella alpina encoding a LPAAT homolog that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant LPAAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.
Abstract translation: 溶血磷脂酰基转移酶（LPAAT）参与石油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 本申请提供了从编码LPAAT同源物的高山被孢霉分离的核酸片段（鉴定为“LPAAT2”），其适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的LPAAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母（Yarrowia lipolytica）中积累具有等于或大于C20链长的长链PUFA。
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50.发明申请Diacylglycerol Acyltransferases for Alteration of Polyunsaturated Fatty Acids and Oil Content in Oleaginous Organisms 有权用于改变多不饱和脂肪酸的二酰基甘油酰基转移酶和油性生物中的油含量公开(公告)号：US20090104674A1
公开(公告)日：2009-04-23
申请号：US12338536
申请日：2008-12-18
Applicant: Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
Inventor： Narendra S. Yadav , Zhixiong Xue , Hongxiang Zhang
IPC: C12P7/64
CPC classification number: C12N9/1029 , C12P7/6463 , C12P7/6472
Abstract: Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
Abstract translation: 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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