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    Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
    1.
    发明授权
    Mutant delta-9 elongases and their use in making polyunsaturated fatty acids 有权
    突变型Δ-9延伸酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US08980589B2

    公开(公告)日：2015-03-17

    申请号：US13218591

    申请日：2011-08-26

    申请人： Michael W. Bostick ,  Hongxian He ,  Yougen Li ,  Quinn Qun Zhu

    发明人： Michael W. Bostick ,  Hongxian He ,  Yougen Li ,  Quinn Qun Zhu

    IPC分类号： C12P21/06 ,  C12P7/64 ,  C12N9/10 ,  C12N15/81

    CPC分类号： C12N9/1029 ,  C12N15/815 ,  C12P7/6472 ,  C12Y203/01016

    摘要： Mutant delta-9 elongases having the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA] and/or α-linolenic [18:3, ALA] to eicosatrienoic acid [20:3, ETrA] are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant delta-9 elongases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant delta-9 elongases in oleaginous yeast are also disclosed.

    摘要翻译： 具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]和/或α-亚麻酸[18：3，ALA]至二十碳三烯酸[20：3]的能力的突变型Δ-9延伸酶 ETrA]。 还公开了分离的核酸片段和包含编码突变型Δ-9延伸酶的这种片段的重组构建物，以及在含油酵母中使用这些突变型Δ-9延长酶制备长链多不饱和脂肪酸的方法[“PUFA”。

    Increased oil content by increasing Yap1 transcription factor activity in oleaginous yeasts
    3.
    发明授权
    Increased oil content by increasing Yap1 transcription factor activity in oleaginous yeasts 有权
    通过增加含油酵母中的Yap1转录因子活性来增加油含量

    公开(公告)号：US08865429B2

    公开(公告)日：2014-10-21

    申请号：US13339461

    申请日：2011-12-29

    申请人： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C12P21/06 ,  C12N1/16 ,  C12P7/64 ,  C07K14/39

    CPC分类号： C07K14/39 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463

    摘要： Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described.

    摘要翻译： 本文描述了具有增加的油含量的转基因含油酵母，其包含增加的Yap1转录因子活性，其中增加的油含量与非转基因油质酵母的油含量进行比较。 通过增加转录因子与能够激活转录因子的蛋白质之间的相互作用，或通过其组合，Yap1转录因子活性的增加来自过表达Yap1转录因子。 还描述了使用这些酵母菌株的方法。

    Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast
    4.
    发明授权
    Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast 有权
    酵母中正链烷烃羟化细胞色素P450启动子区域用于基因表达

    公开(公告)号：US08735094B2

    公开(公告)日：2014-05-27

    申请号：US13437219

    申请日：2012-04-02

    申请人： Quinn Qun Zhu

    发明人： Quinn Qun Zhu

    IPC分类号： C12P21/06 ,  C07H21/02

    CPC分类号： C12N15/815 ,  C12N9/0071

    摘要： Promoter regions associated with the Yarrowia lipolytica n-alkane-hydroxylating cytochrome P450 (ALK2) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.

    摘要翻译： 公开了与解脂耶氏酵母正链烷烃羟化细胞色素P450（ALK2）基因相关的启动子区域，并且已经发现它们在酵母中表达异源基因特别有效。 这些启动子区域可用于驱动涉及ω-3和ω-6脂肪酸生产的基因的高水平表达。

    Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
    5.
    发明授权
    Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08637298B2

    公开(公告)日：2014-01-28

    申请号：US12814815

    申请日：2010-06-14

    申请人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Pamela L. Sharpe ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC分类号： C12N1/00

    CPC分类号： C12N9/1029 ,  A21D2/165 ,  A23K20/158 ,  C12N9/00 ,  C12N9/13 ,  C12N9/93 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481

    摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.

    摘要翻译： 描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其是以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。 除了其他异源Delta9延伸酶，Delta8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶，C16 / 18延长酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A，这些菌株过表达至少一种Δ9延伸酶/Δδ去饱和酶多酶 合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。 至少一种过氧化物酶体生物因子蛋白的表达下调。 要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

    YARROWIA N-ALKANE-HYDROXYLATING CYTOCHROME P450 PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST
    6.
    发明申请
    YARROWIA N-ALKANE-HYDROXYLATING CYTOCHROME P450 PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST 有权
    YARROWIA N-ALKANE-HYOXOXLLA CYTOCHROME P450促进区域用于基因表达在YEAST

    公开(公告)号：US20130089910A1

    公开(公告)日：2013-04-11

    申请号：US13437219

    申请日：2012-04-02

    申请人： Quinn Qun Zhu

    发明人： Quinn Qun Zhu

    IPC分类号： C12N15/81

    CPC分类号： C12N15/815 ,  C12N9/0071

    摘要： Promoter regions associated with the Yarrowia lipolytica n-alkane-hydroxylating cytochrome P450 (ALK2) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.

    摘要翻译： 公开了与解脂耶氏酵母正链烷烃羟化细胞色素P450（ALK2）基因相关的启动子区域，并且已经发现它们在酵母中表达异源基因特别有效。 这些启动子区域可用于驱动涉及ω-3和ω-6脂肪酸生产的基因的高水平表达。

    Δ8 desaturases and their use in making polyunsaturated fatty acids
    8.
    发明授权
    Δ8 desaturases and their use in making polyunsaturated fatty acids 有权
    ＆Dgr; 8去饱和酶及其在制备多不饱和脂肪酸中的用途

    公开(公告)号：US08318463B2

    公开(公告)日：2012-11-27

    申请号：US12825537

    申请日：2010-06-29

    申请人： Howard Glenn Damude ,  Quinn Qun Zhu

    发明人： Howard Glenn Damude ,  Quinn Qun Zhu

    IPC分类号： C12P7/66 ,  C12N15/74 ,  A01N63/00 ,  C07H21/04 ,  A61K48/00

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12N15/8247

    摘要： The present invention relates to Δ8 desaturase genes, which have the ability to convert eicosadienoic acid (EDA; 20:2 ω-6) to dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) and/or eicosatrienoic acid (ETrA; 20:3 ω-3) to eicosatetraenoic acid (ETA; (20:3 ω-3). Isolated nucleic acid fragments and recombinant DNA constructs comprising such fragments encoding Δ8 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ8 desaturases in oleaginous yeast are disclosed.

    摘要翻译： 本发明涉及具有将二十碳二烯酸（EDA; 20：2ω-6）转化为二高-γ-亚麻酸（DGLA; 20：3ω-6）和/或二十碳三烯酸的能力的Dgr .8去饱和酶基因 分离的核酸片段和重组DNA构建体，其包含编码“Dgr。8”去饱和酶的片段，以及一种制备长链多糖的方法， 公开了在含油酵母中使用这些＆Dgr。8去饱和酶的链多不饱和脂肪酸（PUFA）。

    Mutant Δ5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids
    10.
    发明授权
    Mutant Δ5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids 有权
    突变体和Dgr 5在血红素结合基序（HPGG）中突变的去饱和酶及其在制备多不饱和脂肪酸中的用途

    公开(公告)号：US08268598B2

    公开(公告)日：2012-09-18

    申请号：US12562161

    申请日：2009-09-18

    申请人： Quinn Qun Zhu ,  Dana M. Walters Pollak

    发明人： Quinn Qun Zhu ,  Dana M. Walters Pollak

    IPC分类号： C12P7/64 ,  C12N1/20 ,  C12N1/00 ,  C12N1/12 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/0071 ,  C12P7/6427

    摘要： The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed.

    摘要翻译： 本发明涉及具有转化二焦γ-亚麻酸[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且其在细胞色素b5样结构域的HPGG基序内具有至少一个突变。 公开了分离的核酸片段和重组构建体，其包含编码“Dgr。5去饱和酶”的这种片段，以及使用这些突变体＆Dgr。5去饱和酶在含油酵母中制备长链多不饱和脂肪酸[“PUFA”]的方法。