Chalcone isomerase
    44.
    发明授权
    Chalcone isomerase 有权
    查耳酮异构酶

    公开(公告)号:US07202398B2

    公开(公告)日:2007-04-10

    申请号:US10641518

    申请日:2003-08-15

    申请人: Brian McGonigle

    发明人: Brian McGonigle

    摘要: An isolated nucleic acid fragment encoding a chalcone isomerase is disclosed. Also of concern is the synthesis of a recombinant DNA construct encoding all or a portion of the chalcone isomerase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the chalcone isomerase in a transformed host cell.

    摘要翻译: 公开了编码查耳酮异构酶的分离的核酸片段。 还值得关注的是在正义或反义方向上合成编码全部或部分查耳酮异构酶的重组DNA构建体,其中重组DNA构建体的表达导致在转化的宿主细胞中产生改变的查耳酮异构酶水平。

    Sterol biosynthetic enzymes
    46.
    发明授权
    Sterol biosynthetic enzymes 有权
    甾醇生物合成酶

    公开(公告)号:US06545200B1

    公开(公告)日:2003-04-08

    申请号:US09464535

    申请日:1999-12-15

    IPC分类号: A01H300

    CPC分类号: C12N9/90

    摘要: This invention relates to an isolated nucleic acid fragment encoding a sterol biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sterol biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sterol biosynthetic enzyme in a transformed host cell.

    摘要翻译: 本发明涉及编码固醇生物合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或一部分固醇生物合成酶的嵌合基因,其中嵌合基因的表达导致转化的宿主细胞中甾醇生物合成酶水平的变化 。

    Maize glutathione-S-transferase enzymes
    47.
    发明授权
    Maize glutathione-S-transferase enzymes 失效
    玉米谷胱甘肽-S-转移酶

    公开(公告)号:US6096504A

    公开(公告)日:2000-08-01

    申请号:US248335

    申请日:1999-02-10

    CPC分类号: C12N9/1088 C12N15/8274

    摘要: This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of maize glutathione-S-transferase (GST) enzymes involved in the detoxification of xenobiotic compounds in plants and seeds. The invention also relates to the construction of chimeric genes encoding all or a substantial portion of maize GST enzymes, host cells transformed with those genes and methods of the recombinant production of maize GST enzymes. Methods of constructing transgenic plants having altered levels of GST enzymes and screens for identifying maize GST enzyme substrates and maize GST enzyme inhibitor, are also provided.

    摘要翻译: 本发明涉及编码参与植物和种子中异生物化合物解毒的玉米谷胱甘肽-S-转移酶(GST)酶的全部或大部分的分离的核酸片段。 本发明还涉及编码全部或大部分玉米GST酶的嵌合基因的构建,用这些基因转化的宿主细胞和重组生产玉米GST酶的方法。 还提供了构建具有改变的GST酶水平的转基因植物和用于鉴定玉米GST酶底物和玉米GST酶抑制剂的筛选的方法。

    COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS
    48.
    发明申请
    COMPOSITIONS AND METHODS FOR INSECTICIDAL CONTROL OF STINKBUGS 有权
    STINKBUGS的杀虫剂控制组合物和方法

    公开(公告)号:US20110301223A1

    公开(公告)日:2011-12-08

    申请号:US13152795

    申请日:2011-06-03

    摘要: Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

    摘要翻译: 提供了使用沉积元件的方法和组合物,当被害虫摄入时,例如五子科植物害虫或N. viridula,Acrosternum hilare,Piezodorus guildini和/或Halymorpha halys植物害虫,降低靶的表达 序列在害虫中。 在具体实施方案中,靶序列的表达降低控制有害生物,因此方法和组合物能够限制对植物的损害。 本发明提供了SEQ ID NO:1-292或302-304中任一项所述的各种靶多核苷酸或其活性变体和片段,其中目标害虫中一个或多个序列的表达降低控制害虫( 即具有杀虫活性)。 进一步提供的是沉积元件,当受害虫摄入时,降低靶多肽的水平,从而控制害虫。 在具体实施方案中,害虫是五角科。 还提供了包含本发明的沉默元件或其活性变体或片段的植物,植物部分,细菌和其它宿主细胞。

    SULFONYLUREA-RESPONSIVE REPRESSOR PROTEINS
    49.
    发明申请
    SULFONYLUREA-RESPONSIVE REPRESSOR PROTEINS 有权
    SULFONYLUREA-RESPONSIVE REPRESSOR蛋白质

    公开(公告)号:US20110294216A1

    公开(公告)日:2011-12-01

    申请号:US13120895

    申请日:2009-10-22

    摘要: Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.

    摘要翻译: 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽,其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体,载体,原核和真核细胞,以及真核生物,包括包含多核苷酸的植物和种子,和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。