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76 条记录 (耗时 0.056 秒)

    Methods and compositions for direct detection of DNA damage
    42.
    发明授权
    Methods and compositions for direct detection of DNA damage 有权
    直接检测DNA损伤的方法和组成

    公开(公告)号：US08580516B2

    公开(公告)日：2013-11-12

    申请号：US12555668

    申请日：2009-09-08

    申请人： David A. Schwartz ,  Stephen J. Kron

    发明人： David A. Schwartz ,  Stephen J. Kron

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/53

    CPC分类号： G01N33/582 ,  G01N33/5308 ,  G01N2800/40 ,  Y10T436/143333

    摘要： The present invention is a method for detecting the extent of DNA damage in a subject suspected of having DNA damage wherein the damage results in the formation of aldehyde moieties in DNA comprising, obtaining a DNA sample from the subject, combining the DNA sample with a fluorescent, chromogenic, pro-fluorescent or pro-chromogenic hydrazine compound to from a fluorescent DNA, detecting the presence of the fluorescent DNA by monitoring the fluorescent emission and quantitating the fluorescent emission thereby determining the extent of DNA damage in the subject.

    摘要翻译： 本发明是一种检测疑似患有DNA损伤的受试者的DNA损伤程度的方法，其中所述损伤导致在DNA中形成醛部分，其包括：从受试者获得DNA样品，将DNA样品与荧光 ，显色性，前荧光或原色显色肼化合物，通过监测荧光发射和定量荧光发射来检测荧光DNA的存在，从而确定受试者中DNA损伤的程度。

    Methods and/or Use of Oligonucleotide Conjugates Having Varied Degrees of Labeling for Assays and Detections
    43.
    发明申请
    Methods and/or Use of Oligonucleotide Conjugates Having Varied Degrees of Labeling for Assays and Detections 审中-公开
    具有不同标记度的寡核苷酸缀合物的方法和/或用途，用于测定和检测

    公开(公告)号：US20130184184A1

    公开(公告)日：2013-07-18

    申请号：US13302819

    申请日：2011-11-22

    申请人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    发明人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

    摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates having varied degrees of labeling for assays and detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

    摘要翻译： 本公开涉及具有不同程度的标记用于测定和检测以及相关系统和/或试剂盒的寡核苷酸缀合物的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

    Lane marker detection and fitting
    48.
    发明申请
    Lane marker detection and fitting 有权
    车道标记检测和装配

    公开(公告)号：US20080109118A1

    公开(公告)日：2008-05-08

    申请号：US11592824

    申请日：2006-11-03

    申请人： David A. Schwartz ,  Jan K. Schiffmann ,  Lisa R. Hamilton

    发明人： David A. Schwartz ,  Jan K. Schiffmann ,  Lisa R. Hamilton

    IPC分类号： G06F17/00

    CPC分类号： G06K9/4609 ,  G06K9/00798

    摘要： A method of lane marker detection and lane fitting is provided for lane tracking. A lane marker is modeled and split into left and right steps. A filter response is calculated from a cumulative row sum, and normalized for filter pixel size, lane marker brightness and road brightness. A lane marker response is peak detected for positive and negative peaks and checked for having a magnitude above a threshold and being a local peak in a five point neighborhood. A Hough transform is extended to multiple planes to use lane marker features to determine a best line. Lane marker features include a mean and variance of lane marker brightness, lane marker width, lane marker parallelism to a host vehicle direction of travel, and consistence with a predicted lane marker characteristic. A closest lane marker line to a host vehicle is identified, and refitted to account for any curvature.

    摘要翻译： 提供车道跟踪检测方法和车道配合。 车道标记被建模并分为左右步骤。 从累积行和计算滤波器响应，并对滤波器像素大小，车道标记亮度和道路亮度进行归一化。 车道标记响应是正峰值和负峰值的峰值，并且检查幅度高于阈值，并且是五点邻域中的局部峰值。 霍夫变换扩展到多个平面以使用车道标记功能来确定最佳线。 车道标记特征包括道路标记亮度的平均值和方差，车道标记宽度，与主车辆行驶方向的车道标记平行度，以及预测车道标记特征的一致性。 识别到主车辆的最近的车道标记线，并且重新设计以考虑任何曲率。

    Triphosphate oligonucleotide modification reagents and uses thereof
    49.
    发明授权
    Triphosphate oligonucleotide modification reagents and uses thereof 有权
    三磷酸寡核苷酸修饰试剂及其用途

    公开(公告)号：US07173125B2

    公开(公告)日：2007-02-06

    申请号：US10720840

    申请日：2003-11-24

    申请人： David A. Schwartz ,  Richard I. Hogrefe

    发明人： David A. Schwartz ,  Richard I. Hogrefe

    IPC分类号： C07H19/04 ,  C07H19/20 ,  A61K31/70 ,  A01N83/04

    CPC分类号： C07H21/04 ,  C07H19/00 ,  C07H19/04 ,  C07H21/00

    摘要： Hydrazino, oxyamino and carbonyl-based monomers and methods for incorporation into oligonucleotides during enzymatic synthesis are provided. Modified oligonucleotides are provided that incorporate the monomers provided herein. Immobilized oligonucleotides and oligonucleotide conjugates that contain covalent hydrazone or oxime linkages are provided. Methods for preparation of surface bound oligonucleotides are provided. Methods for the preparation of oligonucleotide conjugates are also provided.

    摘要翻译： 提供了肼基，氧基氨基和羰基单体以及在酶合成期间掺入寡核苷酸的方法。 提供了引入本文提供的单体的修饰的寡核苷酸。 提供含有共价腙或肟键的固定寡核苷酸和寡核苷酸缀合物。 提供了制备表面结合寡核苷酸的方法。 还提供了制备寡核苷酸缀合物的方法。