公开(公告)号：US20160178577A1

公开(公告)日：2016-06-23

申请号：US14577511

申请日：2014-12-19

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Bill Maney

IPC: G01N27/447 ,  C12Q1/68 ,  B01L3/00

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

Abstract translation: 公开了分析纳米孔中分子的方法。 通过将纳米孔耦合到电压源，跨越插入膜中的纳米孔施加电压。 纳米孔与电压源分离。 在解耦之后，确定纳米孔上电压的衰减速率。 基于确定的纳米孔上的电压衰减速率，纳米孔中的分子与其它可能的分子区分开来。

公开(公告)号：US20160027728A1

公开(公告)日：2016-01-28

申请号：US14808953

申请日：2015-07-24

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: H01L23/522 ,  G01N27/447 ,  H01L21/768 ,  H01L23/552 ,  H01L23/58

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

公开(公告)号：US20150211060A1

公开(公告)日：2015-07-30

申请号：US14591690

申请日：2015-01-07

Applicant: Genia Technologies, Inc.

Inventor： Kevin Deierling ,  Roger J.A. Chen ,  David J. Fullagar

IPC: C12Q1/68

CPC classification number: G01N27/4473 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721 ,  Y10T436/11 ,  C12Q2565/631 ,  C12Q2565/607

Abstract: A device for controlling, detecting, and measuring a molecular complex is disclosed. The device comprises a common electrode. The device further comprises a plurality of measurement cells. Each measurement cell includes a cell electrode and an integrator electronically coupled to the cell electrode. The integrator measures the current flowing between the common electrode and the cell electrode. The device further comprises a plurality of analog-to-digital converters, wherein an integrator from the plurality of measurement cells is electrically coupled to one analog-to-digital converter of the plurality of analog-to-digital converters.

Abstract translation: 公开了一种用于控制，检测和测量分子复合物的装置。 该装置包括公共电极。 该装置还包括多个测量单元。 每个测量单元包括电池耦合到电池电极的单元电极和积分器。 积分器测量在公共电极和电池电极之间流动的电流。 该装置还包括多个模数转换器，其中来自多个测量单元的积分器电耦合到多个模数转换器的一个模 - 数转换器。

公开(公告)号：US20150011402A1

公开(公告)日：2015-01-08

申请号：US14471051

申请日：2014-08-28

Applicant: GENIA TECHNOLOGIES, INC.

Inventor： Randall W. Davis ,  Roger J.A. Chen

IPC: C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/447 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2525/119 ,  C12Q2525/121 ,  C12Q2525/204 ,  C12Q2527/101 ,  C12Q2563/149 ,  C12Q2565/631

Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.

Abstract translation: 本发明涉及使用纳米孔以获得ss试验DNA中样品DNA序列信息的方法。 该方法包括使用速度凸块来阻断ss测试DNA的随机位置的纳米孔中的ss测试DNA，以获得样品DNA的每个核苷酸的序列信息，并构建样品DNA的整个序列。 本发明还涉及使用由缓冲器促进的纳米孔检测器来鉴定和/或分离具有所需序列的测试DNA。

公开(公告)号：US20140374253A1

公开(公告)日：2014-12-25

申请号：US14334110

申请日：2014-07-17

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: G01N27/447

CPC classification number: G01N27/447 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2527/15 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Techniques for manipulating a molecule in a nanopore embedded in a lipid bilayer are described. In one example, an acquiring electrical stimulus level is applied across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore, a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore is detected, the acquiring electrical stimulus level is changed to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level.

Abstract translation: 描述了在嵌入脂质双层中的纳米孔中操纵分子的技术。 在一个实例中，获得电刺激水平跨越脂质双层施加，其中含有纳米孔的脂质双层的区域的特征在于电阻，并且其中获取电刺激水平倾向于将分子从周围流体吸入纳米孔， 检测到从分子的至少一部分获取到纳米孔中产生的脂质双层的电阻的变化，将获得的电刺激水平改变为保持电刺激水平，其中分子的部分保留在纳米孔中 将获取的电刺激水平改变为保持电刺激水平。

公开(公告)号：US20140027866A1

公开(公告)日：2014-01-30

申请号：US13972616

申请日：2013-08-21

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: H01L23/552

CPC classification number: H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

Abstract translation: 公开了一种具有集成噪声屏蔽的装置。 该装置包括基本上围绕半导体器件的多个垂直屏蔽结构。 该器件还包括在基本上填充有导电流体的半导体器件上方的开口，其中多个垂直屏蔽结构和导电流体屏蔽半导体器件免受环境辐射。 在一些实施例中，该器件还包括在半导体器件下面的导电底部屏蔽，该半导体器件屏蔽半导体器件免受环境辐射。 在一些实施例中，开口被配置为允许生物样品被引入到半导体器件中。 在一些实施例中，垂直屏蔽结构包括多个通孔，其中多个通孔中的每一个将不止一个导电层连接在一起。 在一些实施方案中，所述装置包括纳米孔装置，并且其中所述纳米孔装置包括纳米孔阵列的单个细胞。