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公开(公告)号：US20110122488A1
公开(公告)日：2011-05-26
申请号：US12915921
申请日：2010-10-29
申请人： Thai V. Truong , John M. Choi , Scott E. Fraser , Willy Supatto , David S. Koos
发明人： Thai V. Truong , John M. Choi , Scott E. Fraser , Willy Supatto , David S. Koos
IPC分类号： G02B21/06
CPC分类号： G02B21/16 , G01N21/6408 , G01N21/6428 , G01N21/6458 , G01N21/6486 , G01N2021/655 , G02B21/002 , G02B21/367
摘要： An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal. illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.
摘要翻译： 一种多光子激光荧光与正交结合的多光子光谱显微镜（MP-LISH）的设备和方法。 提供了光片显微镜的照明。 通过对整个果蝇和斑马鱼胚胎的实时成像，MP-LISH的高性能与目前最先进的成像技术相比，保持生物组织内部的良好信号和高空间分辨率（比单光子光更深两倍） 片状显微镜），在采集速度（比常规双光子激光扫描显微镜快超过一个数量级），并且具有低的光毒性。 这种新的成像技术的固有多模态也被证明了二次谐波发生光谱显微镜检测小鼠尾部组织中的胶原蛋白。 共同的，这些属性为MP-LISH在活体生物系统4D成像中的应用提供了广泛的应用。
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公开(公告)号：US07633295B2
公开(公告)日：2009-12-15
申请号：US12109174
申请日：2008-04-24
IPC分类号： G01V3/00
CPC分类号： G01R33/302 , G01R33/31 , G01R33/341 , G01R33/3815 , G01R33/385 , G01R33/4808 , G01R33/5604
摘要： A magnetic resonance (MR) microscope and a dual-mode optic and MR microscope system are disclosed. The MR microscope is provided with a horizontal stage above which a sample to be analyzed is located, a radiofrequency coil assembly located above the horizontal stage and below the sample, a magnetic field gradient module located under the horizontal stage, and a heat exchange unit thermally coupled to the magnetic field gradient module and located under the magnetic field gradient module. The dual-mode optic and MR microscope system is provided with an MR microscope and an optical microscope, the optical microscope comprising a mirror located above the sample.
摘要翻译： 公开了磁共振（MR）显微镜和双模光学和MR显微镜系统。 MR显微镜设置有水平台，在该水平台上，要分析的样品被定位，位于水平台上方和样品下方的射频线圈组件，位于水平台下方的磁场梯度模块和热交换单元 耦合到磁场梯度模块并位于磁场梯度模块下。 双模光学和MR显微镜系统设置有MR显微镜和光学显微镜，光学显微镜包括位于样品上方的反射镜。
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43.发明申请公开(公告)号：US20090214755A1
公开(公告)日：2009-08-27
申请号：US12324605
申请日：2008-11-26
IPC分类号： B05D3/00
CPC分类号： G01N21/7746 , G01N33/54353 , G01N2021/7789
摘要： Micro-cavity resonant sensors have outer surfaces that are functionalized using click chemistry, e.g., involving a cycloaddition reaction of an alkyne functional group and an azide functional group. A first polymer linking element binds to an outer surface of the micro-cavity and has an azide functional group, which bonds to an alkyne functional group of a second polymer linking element as a result of a cycloaddition reaction. A functionalization element such as an antibody, antigen or protein for sensing a target molecule is bound to the second linking element.
摘要翻译： 微腔谐振传感器具有使用点击化学功能化的外表面，例如涉及炔官能团和叠氮官能团的环加成反应。 第一聚合物连接元件与微腔的外表面结合并具有叠氮化物官能团，其结合环加成反应的结果与第二聚合物连接元素的炔官能团结合。 用于感测靶分子的功能化元件如抗体，抗原或蛋白质被结合到第二连接元件。
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公开(公告)号：US20090097031A1
公开(公告)日：2009-04-16
申请号：US12243580
申请日：2008-10-01
IPC分类号： G01N21/00
CPC分类号： G01N21/7746 , G01N21/783 , G01N33/0037 , G01N33/0047 , G01N33/0049 , G01N33/0054 , G01N33/22 , G01N2021/773 , G01N2021/7789 , Y02A50/245 , Y02A50/246 , Y02A50/25
摘要： Micro-cavity gas or vapor sensors and gas or vapor detection methods. Optical energy is introduced into a resonant micro-cavity having a deformable coating such as a polymer. The coating swells or expands when it is exposed to or absorbs a gas or vapor, thereby changing the resonant wavelength of optical energy circulating within the micro-cavity/coating. Expansion or swelling of the coating may be reversible such that it contracts when gas or vapor diffuses from the coating. The coating deformation and/or a change of one or more optical properties of the optical energy circulating within the micro-cavity are used to detect the presence of the gas or vapor or molecules or particulates thereof.
摘要翻译： 微腔气体或蒸气传感器以及气体或蒸气检测方法。 将光能引入具有可变形涂层如聚合物的共振微腔中。 当涂层暴露于或吸收气体或蒸气时，涂层膨胀或膨胀，从而改变在微腔/涂层内循环的光能的共振波长。 涂层的膨胀或膨胀可以是可逆的，使得当气体或蒸汽从涂层扩散时，其膨胀或膨胀。 使用在微腔内循环的光能的涂层变形和/或一个或多个光学特性的变化来检测气体或其蒸气或其分子或微粒的存在。
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45.发明授权公开(公告)号：US07375321B2
公开(公告)日：2008-05-20
申请号：US10502551
申请日：2003-05-07
IPC分类号： G01N13/16
CPC分类号： G01N33/54373
摘要： A bioNEMS device comprises a piezoresistive cantilever having flexing legs of which attach the cantilever to a support and a biofunctionalized portion at the tip. A bias current applied to the legs is limited by a maximal acceptable temperature increase at the biofunctionalized tip. The length of the cantilever has a magnitude chosen to minimize background Johnson noise. A catalyzed receptor on the device binds to a ligand whose binding rate coefficient is enhanced. The catalyst lowers the receptor-ligand binding activation energy and is designed by forced evolution to preferentially bind with the ligand. A carrier signal is injected by a magnetic film disposed on the cantilever which is electromagnetically coupled to a source of the carrier signal. A plurality of NEMS fluidicly coupled transducers generate a plurality of output signals from which a collective output signal is derived, either by averaging or thresholding. The NEMS devices are disposed in microfluidic flow channels and fabricated in a membrane. A linking molecule is attached to the tip of the transducer and a fluffball attached to the linking molecule to increase damping.
摘要翻译： bioNEMS装置包括压阻悬臂，其具有将悬臂连接到支撑件上的弯曲腿和在尖端的生物功能化部分。 施加到腿上的偏置电流受生物功能化尖端的最大可接受的温度升高的限制。 悬臂的长度具有选择的最小化背景约翰逊噪声的大小。 器件上的催化受体与结合速率系数增强的配体结合。 催化剂降低受体 - 配体结合活化能，并通过强制进化来优先与配体结合而设计。 载体信号由布置在悬臂上的磁膜注入，该磁膜电磁耦合到载波信号源。 多个NEMS流体耦合的换能器产生多个输出信号，通过平均或阈值从该输出信号导出集体输出信号。 NEMS装置设置在微流体流动通道中并制成膜。 连接分子连接到换能器的尖端，并且连接到连接分子上的绒毛增加阻尼。
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公开(公告)号：US06962686B2
公开(公告)日：2005-11-08
申请号：US08541191
申请日：1995-10-11
申请人： Jon F. Kayyem , Thomas J. Meade , Scott E. Fraser
发明人： Jon F. Kayyem , Thomas J. Meade , Scott E. Fraser
CPC分类号： A61K49/146 , A61K49/12 , A61K49/189 , B82Y5/00
摘要： A delivery vehicle is described that is capable of being specifically bound to and taken into targeted cells, delivering numerous physiological agents, particularly paramagnetic ions for magnetic resonance imaging (MRI) of the cells. The delivery vehicle comprises a polymeric molecule having a net positive charge complexed with another polymeric molecule having a net negative charge. Cell targeting moieties and physiological agents, including contrast agents and therapeutic agents, are attached to one or both of the polymeric molecules. In one embodiment, the polymeric molecule having a net negative charge is a nucleic acid. Thus, the delivery vehicles can be used in clinical protocols in which nucleic acids for gene therapy and agents for MRI contrast are co-transported to specific cells allowing medical imaging monitoring of nucleic acid delivery.
摘要翻译： 描述了能够特异性结合并进入靶细胞的递送载体，递送许多生理剂，特别是顺磁离子用于细胞的磁共振成像（MRI）。 递送载体包含具有与具有净负电荷的另一个聚合物分子络合的净正电荷的聚合物分子。 细胞靶向部分和生理剂，包括造影剂和治疗剂，连接到一个或两个聚合物分子。 在一个实施方案中，具有净负电荷的聚合物分子是核酸。 因此，递送载体可以用于其中用于基因治疗的核酸和用于MRI对比的药剂共同转运到特定细胞的临床方案，允许核酸递送的医学成像监测。
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公开(公告)号：US06277576B1
公开(公告)日：2001-08-21
申请号：US09306768
申请日：1999-05-07
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07H23/00 , C12Q1/6818 , C12Q1/6825 , Y02A50/52 , C12Q2565/607 , C12Q2565/401 , C12Q2563/137 , C12Q2563/113 , C12Q2565/101 , C12Q2565/1015
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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公开(公告)号：US06268150B1
公开(公告)日：2001-07-31
申请号：US09459751
申请日：1999-12-10
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07H23/00 , C12Q1/6818 , C12Q1/6825 , Y02A50/52 , C12Q2565/607 , C12Q2565/401 , C12Q2563/137 , C12Q2563/113 , C12Q2565/101 , C12Q2565/1015
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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公开(公告)号：US06238870B1
公开(公告)日：2001-05-29
申请号：US09458187
申请日：1999-12-08
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07H23/00 , C12Q1/6818 , C12Q1/6825 , Y02A50/52 , C12Q2565/607 , C12Q2565/401 , C12Q2563/137 , C12Q2563/113 , C12Q2565/101 , C12Q2565/1015
摘要： The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
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公开(公告)号：US06232295B1
公开(公告)日：2001-05-15
申请号：US08321552
申请日：1994-10-12
IPC分类号： A61K4800
CPC分类号： A61K49/146 , A61K47/645 , A61K49/085 , Y10S977/808 , Y10S977/927 , Y10S977/93
摘要： A delivery vehicle is described that is capable of being specifically bound to and taken into targeted cells, delivering numerous paramagnetic ions for magnetic resonance imaging (MRI) of the cells. The delivery vehicle comprises a polymeric molecule having a net positive charge complexed with another polymeric molecule having a net negative charge. Cell targeting moieties and MRI contrast agents are attached to one or both of the polymeric molecules. In one embodiment, the polymeric molecule having a net negative charge is a nucleic acid. Thus, the delivery vehicles can be used in clinical protocols in which nucleic acids for gene therapy and agents for MRI contrast are co-transported to specific cells allowing medical imaging monitoring of nucleic acid delivery.
摘要翻译： 描述了能够特异性结合并进入目标细胞的递送载体，为细胞的磁共振成像（MRI）递送许多顺磁离子。 递送载体包含具有与具有净负电荷的另一个聚合物分子络合的净正电荷的聚合物分子。 细胞靶向部分和MRI造影剂连接到一个或两个聚合物分子。 在一个实施方案中，具有净负电荷的聚合物分子是核酸。 因此，递送载体可以用于其中用于基因治疗的核酸和用于MRI对比的药剂共同转运到特定细胞的临床方案，允许对核酸递送进行医学成像监测。
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