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公开(公告)号：US5985557A
公开(公告)日：1999-11-16
申请号：US756386
申请日：1996-11-26
IPC分类号： G01N33/50 , C12N1/21 , C12N9/12 , C12N9/16 , C12N9/22 , C12N15/09 , C12Q1/68 , G01N33/566 , C07H21/02
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US5871911A
公开(公告)日：1999-02-16
申请号：US385629
申请日：1995-02-09
IPC分类号： C07H21/00 , C07K14/47 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , G01N33/53 , C07H21/04 , C12N15/00 , C12P19/34
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , C12Q1/705
摘要： A method of cleaving a target nucleic acid molecule is disclosed. A cleavage structure is formed that comprises the target nucleic acid and a pilot nucleic acid. A first region of the target nucleic acid is annealed to the pilot nucleic acid to form a duplex structure. A second region of the target nucleic acid contiguous to the duplex is not annealed to the pilot nucleic acid, thus forming a junction site between the duplex region and the non-annealed region. The cleavage structure is exposed to a cleavage agent capable of preferentially cleaving the cleavage structure at a target site in a manner independent of the sequence of the cleavage structure. The cleavage structure and the cleavage agent are incubated under conditions wherein cleavage can occur.
摘要翻译： 公开了切割靶核酸分子的方法。 形成包含靶核酸和导向核酸的切割结构。 靶核酸的第一区域与引导核酸退火以形成双链体结构。 与双链体相邻的靶核酸的第二区域不与引导核酸退火，从而在双相区域和非退火区域之间形成连接位点。 裂解结构暴露于能够以与切割结构的序列无关的方式在靶位点优先切割切割结构的切割剂。 裂解结构和切割剂在可以发生切割的条件下孵育。
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公开(公告)号：US07829314B2
公开(公告)日：2010-11-09
申请号：US12476912
申请日：2009-06-02
IPC分类号： C12P19/34
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US07790393B2
公开(公告)日：2010-09-07
申请号：US12174277
申请日：2008-07-16
CPC分类号： G06Q30/06 , G06Q10/087
摘要： The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.
摘要翻译： 本发明提供用于开发和优化用于基础研究，临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。 特别地，本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。 本发明还提供优化多重扩增反应的方法。
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公开(公告)号：US07785852B2
公开(公告)日：2010-08-31
申请号：US11198675
申请日：2005-08-05
CPC分类号： C12N9/1252 , C12Q1/682 , C12Q1/6846 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
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公开(公告)号：US07691573B2
公开(公告)日：2010-04-06
申请号：US11198657
申请日：2005-08-05
CPC分类号： C12N9/1252 , C12Q1/682 , C12Q1/6846 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
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47.发明申请COMPOSITIONS AND METHODS FOR ANALYSIS OF NUCLEIC ACID MOLECULES DURING AMPLIFICATION REACTIONS 审中-公开放大反应期间核酸分子分析的组成和方法公开(公告)号：US20090253142A1
公开(公告)日：2009-10-08
申请号：US12404240
申请日：2009-03-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6823 , C12Q1/6827 , C12Q1/6851 , C12Q2525/161 , C12Q2521/313 , C12Q2561/109 , C12Q2525/301 , C12Q2531/113 , C12Q2537/149 , C12Q2561/113 , C12Q2565/1015
摘要： The present invention provides systems, methods and kits for performing a detection assay (e.g., invasive cleavage assay) in combination with an amplification assay (e.g., PCR), where the detection assay employs enzyme footprint probes with relatively short (e.g., 6-12 bases) analyte-specific regions configured to provide a preferred footprint length of duplex for use with a particular nucleic acid modifying enzyme. In some embodiments, such assays are used for target quantification, and in other embodiments, such assays are used for genotyping. In certain embodiments, the use of such short probes allows for assays with increased dynamic range.
摘要翻译： 本发明提供了用于与扩增测定（例如PCR）结合进行检测测定（例如侵入性切割测定）的系统，方法和试剂盒，其中检测测定使用相对较短的酶标记探针（例如6-12 碱基）分析物特异性区域，其被配置为提供与特定核酸修饰酶一起使用的双链体的优选覆盖物长度。 在一些实施方案中，这种测定用于靶定量，并且在其它实施方案中，这种测定用于基因分型。 在某些实施方案中，使用这种短探针允许具有增加的动态范围的测定。
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48.发明授权Kits for detection of nucleic acids using invasive cleavage structures and flap endonucleases 有权用于使用侵入性切割结构和瓣内切酶检测核酸的试剂盒公开(公告)号：US07514220B2
公开(公告)日：2009-04-07
申请号：US11926062
申请日：2007-10-28
申请人： Jeff G. Hall , Victor I. Lyamichev , Andrea L. Mast , Mary Ann D. Brow , Robert W. Kwiatkowski , Stephanie H. Vavra
发明人： Jeff G. Hall , Victor I. Lyamichev , Andrea L. Mast , Mary Ann D. Brow , Robert W. Kwiatkowski , Stephanie H. Vavra
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/683 , C12Q1/701 , C12Q1/703 , C12Q1/706 , Y10S435/81 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整的和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。
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公开(公告)号：US07407782B2
公开(公告)日：2008-08-05
申请号：US11103943
申请日：2005-04-12
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20080182254A1
公开(公告)日：2008-07-31
申请号：US11926062
申请日：2007-10-28
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/683 , C12Q1/701 , C12Q1/703 , C12Q1/706 , Y10S435/81 , C12Q2561/109
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中各种病毒的核酸的敏感和具体方法。
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