公开(公告)号：US20120273674A1

公开(公告)日：2012-11-01

申请号：US13094146

申请日：2011-04-26

申请人： Melvin Andrew Park ,  Jochen Franzen

发明人： Melvin Andrew Park ,  Jochen Franzen

IPC分类号： H01J49/26 ,  H01J49/10

CPC分类号： G01N27/626

摘要： In an ion mobility spectrometer in which a gas pushes ions along a spectrometer axis against and over an electrical field barrier, the electric field barrier is generated with a plateau of slightly increasing height along the axis of the spectrometer. Alternately, the electric filed barrier may have a plateau with constant height, but the gas flow decreases in velocity along the axis of the spectrometer in the vicinity of the plateau.

摘要翻译： 在离子迁移谱仪中，其中气体沿着光谱仪轴线推动离子抵抗和超过电场屏障，电场屏障是沿着光谱仪轴线的高度稍微增加的高原产生的。 或者，电场屏障可以具有恒定高度的平台，但气流在平台附近沿着光谱仪的轴线的速度减小。

公开(公告)号：US20110062322A1

公开(公告)日：2011-03-17

申请号：US12883885

申请日：2010-09-16

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： B01D59/44 ,  G01N27/62

CPC分类号： G01N27/622

摘要： A supersonic gas jet having gas molecules with substantially equal velocities is formed by directing the gas through a Laval nozzle into an evacuated chamber. A field barrier having a substantially constant height across a cross-section of the supersonic gas jet is formed by respectively applying potentials U2, U3 and U4 to an arrangement of three apertured diaphragms R2, R3 and R4, which are respectively separated by distances d2 and d3, where (U4−U3)/(U3−U2)=d3/d2. The ions in the supersonic gas jet are directed against the field barrier, where ions with a mobility below a mobility threshold are pushed over the field barrier, and where ions with a mobility higher than the mobility threshold are held back by the field barrier.

摘要翻译： 具有基本相同速度的气体分子的超音速气体射流通过将气体通过拉瓦尔喷嘴引导到抽空的室中而形成。 通过分别将电位U2，U3和U4施加到三个有孔隔膜R2，R3和R4的排列上，形成跨超声波气体射流横截面具有基本恒定高度的场屏障，它们分别以距离d2和 d3，其中（U4-U3）/（U3-U2）= d3 / d2。 超音速气体射流中的离子被引导到场屏障，其中迁移率低于迁移率阈值的离子被推到场屏障上，并且其中迁移率高于迁移率阈值的离子被场屏障阻挡。

公开(公告)号：US20110039350A1

公开(公告)日：2011-02-17

申请号：US12853637

申请日：2010-08-10

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： G01N1/00 ,  H01J49/04 ,  H01J49/14 ,  H01J49/16 ,  H01J49/10 ,  H01J49/12 ,  B01J19/08

CPC分类号： H01J49/0431 ,  Y10T436/25

摘要： Gaseous analyte molecules are ionized at atmospheric pressure and provided to an inlet capillary of an ion spectrometer vacuum system by passing the ions through a reaction tube that ends in a conical intermediate piece for a gastight and smooth transition into the inlet capillary. The reaction tube is shaped so that the atmospheric pressure gas stream passing therethrough form the entrance of the tune to the intermediate piece is stably laminar. Analyte molecules from gas chromatographs, spray devices or vaporization devices can be introduced into the entrance of the reaction tube and ionized within the tube by single- or multi-photon ionization, by chemical ionization, by reactant ions or by physical ionization. For single- or multi-photon ionization, a beam from a laser can be passed axially down the reaction tube. Reactant ions can be produced by any means outside of the reaction tube and mixed with the analyte molecules within the tube.

摘要翻译： 气态分析物分子在大气压下离子化，并通过使离子通过端接在锥形中间件中的反应管提供给离子光谱仪真空系统的入口毛细管，以便气密和平滑过渡到入口毛细管中。 反应管成形为使得通过其的大气压力气流形成曲调到中间件的入口是稳定的层流的。 来自气相色谱仪，喷雾装置或蒸发装置的分析物分子可以通过单光子或多光子电离，通过化学电离，反应物离子或物理电离引入反应管的入口并离子化。 对于单光子或多光子电离，来自激光束的光束可以沿着反射管轴向通过。 反应物离子可以通过反应管外的任何方式产生并与管内的分析物分子混合。

公开(公告)号：US20110036974A1

公开(公告)日：2011-02-17

申请号：US12857161

申请日：2010-08-16

申请人： Jochen Franzen ,  Claus Köster

发明人： Jochen Franzen ,  Claus Köster

IPC分类号： H01J49/04

CPC分类号： H01J49/062 ,  H01J49/0445

摘要： Charged droplets are guided along a defined path from a droplet source to a droplet sink. A focusing pseudopotential distribution generated by audio frequencies on electrodes of a guiding device guide the charged droplets from the droplet source to the droplet sink with low loss. The droplets can be driven along the droplet guide by a gas flow, an axial electric field or a combination of both. For example, charged droplets from a spray capillary of an electrospray ion source at atmospheric pressure may be introduced into the inlet capillary leading to the vacuum system of ion analyzers, a procedure similar to that used up to now in nanoelectrospraying, but with substantially higher flow rates. In the guiding device, the droplets can be manipulated in different ways, for example evaporated down to a desired size. The introduction of small droplets into gas-aspirating capillaries is of interest because it is possible to keep the droplets on axis by Bernoulli focusing and to guide them in large quantities and with low loss through the capillary. The ability to guide the droplets makes it also possible to install a segmented inlet capillary with intermediate pumping, which allows pumping capacity to be saved. Advantageously, the sensitivity of ion analyzers such as mass spectrometers or ion mobility spectrometers by at least one order of magnitude.

摘要翻译： 带电液滴沿着从液滴源到液滴槽的限定路径被引导。 由引导装置的电极上的音频产生的聚焦伪势分布将带电液滴从液滴源引导到具有低损耗的液滴槽。 液滴可以通过气流，轴向电场或两者的组合沿液滴引导件驱动。 例如，在大气压下来自电喷雾离子源的喷雾毛细管的带电液滴可以引入入口毛细管中，导入离子分析仪的真空系统，该方法与迄今为止在纳电电喷雾中使用的方法类似，但具有显着更高的流动 价格。 在引导装置中，液滴可以以不同的方式进行操作，例如蒸发到所需尺寸。 将小液滴引入吸气毛细管是令人感兴趣的，因为可以通过伯努利聚焦将液滴保持在轴上，并且通过毛细管大量引导并以低损耗引导。 引导液滴的能力使得还可以安装具有中间泵送的分段入口毛细管，这允许节省泵送能力。 有利地，诸如质谱仪或离子迁移谱仪的离子分析仪的灵敏度至少有一个数量级。

公开(公告)号：US20100224775A1

公开(公告)日：2010-09-09

申请号：US12716813

申请日：2010-03-03

申请人： Andreas Haase ,  Jens Höhndorf ,  Jochen Franzen

发明人： Andreas Haase ,  Jens Höhndorf ,  Jochen Franzen

IPC分类号： H01J49/26 ,  H01J49/02

CPC分类号： H01J49/164 ,  H01J49/0045

摘要： Mass spectrometry with lasers generates ions from analyte molecules by matrix assisted laser desorption for a variety of different mass spectrometric analysis procedures. The mass spectrometers with laser systems supply laser light pulses having at least two different pulse durations, and mass spectrometric measuring techniques use the laser light pulses of different durations. The duration of the laser light pulses allows the characteristics of the ionization of the analyte molecules, particularly the occurrence of the ISD (in-source decay) and PSD (post-source decay) types of fragmentation, whose fragment ion spectra supply different kinds of information, to be adapted to the analytic procedure.

摘要翻译： 使用激光的质谱法通过基质辅助激光解吸从分析物分子产生离子，用于各种不同的质谱分析程序。 具有激光系统的质谱仪提供具有至少两个不同脉冲持续时间的激光脉冲，并且质谱测量技术使用不同持续时间的激光脉冲。 激光脉冲的持续时间允许分析物分子的电离特性，特别是ISD（源内衰变）和PSD（后源衰变）类型的断裂的出现，其碎片离子谱提供不同种类的 信息，以适应分析程序。

公开(公告)号：US07595486B2

公开(公告)日：2009-09-29

申请号：US11691166

申请日：2007-03-26

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： H01J49/04

CPC分类号： H01J49/065 ,  H01J49/063

摘要： In a multipole rod ion guide system operated with RF voltages to collect or transmit ions, the inhomogeneity of the electric RF fields is increased in front of the ion guide rods by forming the rod surfaces from a plurality of spaced electrodes. The inhomogeneous fields produced by the plurality of electrodes increases the mass range over which the ions are guided effectively while still maintaining a pseudopotential minimum which is as well defined as possible close to the axis. Particularly favorable ion guides of this type make it possible to apply an axial DC field to the guide system for the active transport of the ions.

摘要翻译： 在用RF电压操作以收集或传输离子的多极杆离子导向系统中，通过从多个间隔开的电极形成杆表面，在离子导杆的前方增加了电RF场的不均匀性。 由多个电极产生的不均匀场增加离子被有效引导的质量范围，同时仍保持尽可能靠近轴线限定的赝势最小值。 这种特别有利的离子导向器使得可以将轴向DC场施加到导向系统以有源输送离子。

公开(公告)号：US07399640B2

公开(公告)日：2008-07-15

申请号：US10828153

申请日：2004-04-20

申请人： Martin Schürenberg ,  Jochen Franzen

发明人： Martin Schürenberg ,  Jochen Franzen

IPC分类号： G01N24/00 ,  B01J19/08 ,  H01J49/40

CPC分类号： H01J49/0418 ,  B01L3/5085 ,  G01N1/40 ,  Y10T436/11 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/2575

摘要： The invention relates to sample support plates with hydrophilic anchors in a strongly hydrophobic environment for mass spectroscopic analysis of biosubstances with ionization by matrix-assisted laser desorption and ionization (MALDI), procedures for manufacturing these sample supports, and connected procedures for loading these sample supports with biomolecular samples. The invention provides areas with affinity adsorbents adjacent to the hydrophilic anchors for purifying biosubstances and, if wanted, for performing an affinity selection of biosubstances, whereby the finally prepared matrix sample crystals with the biosubstances for the MALDI analysis are well localized on the hydrophilic anchors.

摘要翻译： 本发明涉及在强疏水环境中具有亲水性锚定物的样品支撑板，用于通过基质辅助激光解吸和电离（MALDI）进行离子化生物物质的质谱分析，制备这些样品载体的方法以及用于加载这些样品载体的连接方法 与生物分子样品。 本发明提供了亲水性吸附剂的邻近亲水性锚定物的区域，用于纯化生物条件，并且如果需要，进行生物物质的亲和选择，由此最终制备的具有用于MALDI分析的生物基质的基质样品晶体很好地定位在亲水性锚定体上。

公开(公告)号：US20070272848A1

公开(公告)日：2007-11-29

申请号：US11695796

申请日：2007-04-03

申请人： Jochen Franzen

发明人： Jochen Franzen

IPC分类号： B01D59/44

CPC分类号： H01J49/04 ,  H01J49/004 ,  H01J49/401 ,  H01J49/4225

摘要： In a time-of-flight mass spectrometer with orthogonal ion injection performed by a pulser to which the ions are fed by an RF ion guide, compensation is provided for mass discrimination that occurs when the ions are injected into the pulser. This is accomplished by designing at least a part of the ion guide as an ion storage device, by emptying the filled ion storage device mass-selectively in ion groups, group-by-group, and by serially feeding the ion groups to the pulser with correct timing, using the mass selectivity of the pulser filling process to compensate for the mass discrimination.

摘要翻译： 在由离子由RF离子导向器馈送的脉冲发生器进行的具有正交离子注入的飞行时间质谱仪中，提供了当离子注入脉冲发生器时发生的质量鉴别的补偿。 这是通过将离子导向器的至少一部分设计为离子存储装置来实现的，通过以离子基团的形式大量选择性地排空填充的离子存储装置，并且通过将离子基团串联馈送到脉冲发生器， 正确的时间，使用脉冲发生器填充过程的质量选择性来补偿质量鉴别。

公开(公告)号：US07196326B2

公开(公告)日：2007-03-27

申请号：US11149952

申请日：2005-06-10

申请人： Jochen Franzen ,  Evgenij Nikolaev

发明人： Jochen Franzen ,  Evgenij Nikolaev

IPC分类号： B01D59/44 ,  H01J49/42 ,  H01J49/04

CPC分类号： H01J49/062 ,  H01J49/0077 ,  H01J49/0095

摘要： The invention relates to a reaction cell for reactions between different types of ion species and a related mass spectrometer to analyze the ion products. The invention consists in an RF-operated straight ion guide with a side inlet, particularly suitable for reactions between positive and negative ion species, one ion species being fed in through the side inlet. Particularly favorable is an ion guide made up of a set of coaxial apertured diaphragms with a slight axial potential gradient. The reactions can be used for a fragmentation of multiply charged protein or peptide ions by electron transfer, or for the removal of excess charges of multiply charged biopolymer ions, for example.

摘要翻译： 本发明涉及用于不同类型离子种类之间的反应的反应池和相关的质谱仪来分析离子产物。 本发明包括具有侧入口的RF操作的直离子导向器，特别适用于正离子和负离子之间的反应，一个离子种类通过侧入口进料。 特别有利的是由具有轻微轴向电位梯度的一组同轴孔径隔膜组成的离子导向器。 该反应可以用于例如通过电子转移分离多电荷蛋白质或肽离子，或用于除去多电荷生物聚合物离子的过量电荷。

公开(公告)号：US07164125B2

公开(公告)日：2007-01-16

申请号：US11090316

申请日：2005-03-25

申请人： Gerhard Weiss ,  Jochen Franzen ,  Carsten Stoermer

发明人： Gerhard Weiss ,  Jochen Franzen ,  Carsten Stoermer

IPC分类号： H01J49/42

CPC分类号： H01J49/04 ,  H01J49/443

摘要： The invention relates to two-dimensional quadrupole systems along whose axis an axial DC field is superimposed. The invention involves coating the hyperbolic or cylindrical surfaces of quadrupole systems with thin insulating layers and metal films thereupon and generating axial potential gradients or saddle ramps using appropriate electrical supply of DC potentials and superimposed RF voltages to the metal films. Systems of this type can be used in a plurality of ways, ranging from mass filters with high transmission to fragmentation cells with extremely low ion losses.

摘要翻译： 本发明涉及二维四极杆系统，其轴上的轴向DC场被叠加。 本发明涉及使用薄的绝缘层和金属膜涂覆四极杆系统的双曲面或圆柱形表面，并使用合适的DC电势和叠加的RF电压产生轴向电位梯度或鞍形斜坡。 这种类型的系统可以以多种方式使用，从具有高透射率的质量过滤器到具有极低离子损失的碎裂单元。