公开(公告)号：US5981183A

公开(公告)日：1999-11-09

申请号：US821782

申请日：1997-03-20

申请人： Yutaka Takarada ,  Hiroaki Inoue ,  Shuji Shibata ,  Yoshihisa Kawamura

发明人： Yutaka Takarada ,  Hiroaki Inoue ,  Shuji Shibata ,  Yoshihisa Kawamura

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6865

摘要： In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.

摘要翻译： 在扩增特异性核酸序列的方法中，可以进行非特异性杂交的可能性低的高度特异性扩增。 还提供了稳定的试剂，其供应和储存的活性不降低。 热稳定酶用作基于复制RNA的扩增系统所需的RNA依赖性DNA聚合酶，DNA依赖性DNA聚合酶，DNA依赖性RNA聚合酶和核糖核酸酶H。 特别优选共同使用具有RNA依赖性DNA聚合酶活性，DNA依赖性DNA聚合酶活性和核糖核酸酶H活性的嗜热栖热菌的热稳定性酶和耐热性DNA依赖型RNA聚合酶。 通过这种方法，通过使用热稳定酶来防止酶的失活，并且可以不依次加入酶进行扩增。

公开(公告)号：US5773585A

公开(公告)日：1998-06-30

申请号：US667357

申请日：1996-06-21

申请人： Noriyuki Hongo ,  Shizuo Hattori ,  Kazumi Yamamoto ,  Shinichi Teshima ,  Yoshihisa Kawamura

发明人： Noriyuki Hongo ,  Shizuo Hattori ,  Kazumi Yamamoto ,  Shinichi Teshima ,  Yoshihisa Kawamura

IPC分类号： C12N9/06 ,  C12Q1/32 ,  C12N9/02

CPC分类号： C12Q1/32 ,  C12N9/0016 ,  C12Y104/01002 ,  G01N2333/906 ,  Y10S435/874

摘要： A novel glutamate dehydrogenase is derived from the genus Pseudomonas and is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, has an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP.

摘要翻译： 新型谷氨酸脱氢酶源自假单胞菌属，对L-谷氨酸特异性，需要NAD +和NADH作为辅酶，不对NADP +和NADPH起作用，具有最佳pH 10.5-11.5（氧化脱氨），pH稳定性 5-10（25℃; 20小时），最适温度约60℃（氧化脱氨），分子量约280,000（凝胶过滤）或约41,000（SDS-PAGE），未活化 由ADP。

公开(公告)号：US5333736A

公开(公告)日：1994-08-02

申请号：US974739

申请日：1992-11-13

申请人： Yoshihisa Kawamura

发明人： Yoshihisa Kawamura

IPC分类号： B65B31/04 ,  B65D30/24 ,  B65D33/16 ,  B65D81/20 ,  B65D85/07 ,  B65D85/18

CPC分类号： B65D81/2038 ,  B65D33/16

摘要： A self-sealing compression packaging bag for compression sealing of an article on evacuation and a compression packaging kit using the bag. Clothing is introduced at an opening part of a bag formed of a film material exhibiting self-adherent properties and provided with an evacuating port. An evacuating tube is introduced into the evacuating port and the inside of the bag is evacuated through the tube by a vacuum pump. The opening part is automatically sealed at this time, and the evacuation port is automatically sealed on extracting the evacuating tube. Tight sealing of the bag may be achieved automatically on evacuation without the need for heat compression bonding. Also, the bag is portable.

摘要翻译： 一种用于在抽空时对物品进行压缩密封的自密封压缩包装袋和使用该袋的压缩包装套件。 服装被引入由显示自粘性的薄膜材料形成的袋的开口部分并且设置有排气口。 抽真空管被引入排气口，袋子的内部通过真空泵通过管抽真空。 此时自动密封开口部，抽真空管自动密封。 袋子的紧密密封可以在排空时自动实现，而不需要热压接。 此外，该袋是便携式的。

公开(公告)号：US5309606A

公开(公告)日：1994-05-10

申请号：US483486

申请日：1992-11-30

申请人： Yoshihisa Kawamura

发明人： Yoshihisa Kawamura

IPC分类号： A44B11/10 ,  A44B11/25 ,  A44B11/06

CPC分类号： A44B11/10 ,  A44B11/2557 ,  Y10T24/4019 ,  Y10T24/4084

摘要： A tongue assembly for use in a seat belt device is formed of a tongue main body engaging a buckle and a slider slidably disposed over the main body. A first slit is situated in a middle portion of the main body to extend in a direction perpendicular to an inserting direction of the main body relative to the buckle. A first wall portion is fixed to the main body to project upwardly from an upper surface of the main body along the first slit at a side of a rear portion. A second wall portion is formed in an upper side of the slider and has a surface opposing to the first wall portion. A second slit is formed at a lower side of the slider so that a rear edge of the second slit at a side of a rear portion of the main body is situated close to an insertion portion of the main body with respect to the surface of the second wall. A third slit is further formed in the rear portion of the main body. A seat belt extends between the first and second wall portions, through the first and second slits, under the lower side of the slider and through the third slit so that when a tension is applied from the side of the third slit, the slider is pulled toward the rear portion of the main body to urge the second wall portion of the slider to the first wall portion to thereby strongly hold the seat belt between the first and second wall portions.

摘要翻译： 用于安全带装置的舌部组件由与扣环接合的舌本体和可滑动地设置在主体上的滑块形成。 第一狭缝位于主体的中间部分中，以在与主体相对于带扣的插入方向垂直的方向上延伸。 第一壁部分固定在主体上，从主体的上表面沿着第一狭缝在后部的一侧向上突出。 第二壁部形成在滑块的上侧，并且具有与第一壁部相对的表面。 第二狭缝形成在滑块的下侧，使得主体后部侧的第二狭缝的后边缘相对于主体的表面位于主体的插入部分附近 第二墙。 第三狭缝还形成在主体的后部。 安全带在第一和第二壁部分之间延伸穿过第一和第二狭缝，在滑块的下侧下方并且穿过第三狭缝，使得当从第三狭缝侧施加张力时，拉动滑块 朝向主体的后部推动滑块的第二壁部分到第一壁部分，从而将安全带牢固地保持在第一和第二壁部之间。

公开(公告)号：US4669440A

公开(公告)日：1987-06-02

申请号：US437680

申请日：1982-10-29

申请人： Sadao Takase ,  Yoshihisa Kawamura ,  Toyoaki Nakagawa

发明人： Sadao Takase ,  Yoshihisa Kawamura ,  Toyoaki Nakagawa

IPC分类号： F02B3/06 ,  F02D41/20 ,  F02D41/40 ,  F02M65/00 ,  F02M39/00

CPC分类号： F02D41/401 ,  F02D41/20 ,  F02M65/005 ,  F02B3/06 ,  F02D2200/063 ,  F02D41/2096 ,  Y02T10/44

摘要： A fuel injection detecting system is used with a diesel engine having a fuel injection nozzle, the nozzle including a movable member for effecting fuel injection into the engine when the valve member is displaced from its normal position and interrupting fuel injection when the valve member is returned to its normal position. The detecting system includes a sensor for sensing displacement of the valve member and generating a signal indicative thereof. A comparator is provided to compare the signal from the sensor with a reference signal and to generate a signal indicative of fuel injection via the nozzle. The detecting system also includes a device for preventing the fuel injection signal from including components unrelated to fuel injection via the nozzle. This device may disable the sensor for a predetermined period immediately after the first major displacement of the valve member. Alternatively, the device may adjust the reference voltage so as to hold the voltage thereof at a level exceeding that of all features of the sensor signal except the signal largest peak during each fuel injection.

摘要翻译： 燃料喷射检测系统与具有燃料喷射喷嘴的柴油发动机一起使用，该喷嘴包括可动构件，用于当阀构件从其正常位置移位时进行燃料喷射并在阀构件返回时中断燃油喷射 到正常位置。 检测系统包括用于感测阀构件的位移并产生指示其的信号的传感器。 提供比较器以将来自传感器的信号与参考信号进行比较，并产生指示经由喷嘴的燃料喷射的信号。 检测系统还包括用于防止燃料喷射信号包括经由喷嘴与燃料喷射无关的部件的装置。 该装置可以在阀构件的第一主要位移之后立即禁用传感器达预定的时间。 或者，器件可以调整参考电压，以便在每次燃料喷射期间除了信号最大峰值之外，将其电压保持在超过传感器信号的所有特征的电压。

公开(公告)号：US4662330A

公开(公告)日：1987-05-05

申请号：US743673

申请日：1985-06-11

申请人： Giichi Shioyama ,  Kunihiko Sugihara ,  Yukihiro Etoh ,  Toshiaki Tanaka ,  Yoshihisa Kawamura

发明人： Giichi Shioyama ,  Kunihiko Sugihara ,  Yukihiro Etoh ,  Toshiaki Tanaka ,  Yoshihisa Kawamura

IPC分类号： F02B3/06 ,  F02B19/08 ,  F02B19/14 ,  F02B23/04

CPC分类号： F02B23/04 ,  F02B19/08 ,  F02B19/14 ,  F02B3/06 ,  Y02T10/125

摘要： In order to promote a good flame dispersion within the combustion chamber of a swirl chamber type Diesel engine, a shaped recess formed in the piston crown is arranged to have a trench portion into which the flame from the swirl chamber is injected and mirror image flame dispersion portions on either side of the trench which terminates at the periphery of the piston crown. This arrangement inhibits the formation of secondary flame flows which tend impede desirable patterns within the flame disportion portions and simultaneously enlarges the dispersion area. In addition, careful selection of the dimensions of the transfer port which interconnects the swirl chamber and the main combustion chamber, and the various sections of the shaped recess, promotes further reductions in HC and smoke emissions.

摘要翻译： 为了在涡流室型柴油发动机的燃烧室内促进良好的火焰分散，形成在活塞冠部中的成形凹部被布置成具有沟槽部分，来自涡流室的火焰被注入到该沟槽部分中，并且镜像火焰分散 沟槽两侧的部分终止于活塞顶部的周边。 这种布置抑制二次火焰流的形成，这阻碍了火焰不均匀部分内的期望图案，并同时扩大了分散面积。 另外，仔细选择将涡流室和主燃烧室相互连接的输送口的尺寸以及成形凹槽的各个部分的尺寸，进一步降低了HC和烟雾的排放。

公开(公告)号：US4640249A

公开(公告)日：1987-02-03

申请号：US749413

申请日：1985-06-27

申请人： Yoshihisa Kawamura ,  Shuzo Fukuzumi

发明人： Yoshihisa Kawamura ,  Shuzo Fukuzumi

IPC分类号： F02P5/145 ,  F02P5/152 ,  F02P5/15

CPC分类号： F02P5/1521 ,  F02P5/1455 ,  Y02T10/46

摘要： A system and method for controlling an ignition timing in an internal combustion engine which controls the ignition timing by correcting the ignition timing by a retardation angle for avoiding the occurrence of the knocking when the level of knocking exceeds a predetermined angle and/or by correcting the ignition timing (MBT control) by a correction value on the basis of an crank angle at which the combustion chamber pressure has reached its maximum. When the engine is not in a transient operating state, the system stores the correction value of the ignition timing during knocking avoidance control or during MBT control as a learning value corresponding to the engine operating condition and calculates the actual ignition timing on the basis of the learning value and basic ignition timing determined by the engine operating condition. When the engine is in the transient operating state, the MBT control is not carried out. Therefore, such an undesirable state occurs that the correction value of the ignition timing is varied to a large degree when the engine operating state is changed, thus torque variation results, and inaccurate ignition timing control is not carried out. In addition, since the history of retardation angle correction by means of the past knocking avoidance control reflects the present calculation of ignition timing angle value, a preventive control of the occurrence of knocking is achieved. Consequently, a driving range in which the MBT control becomes effective is extended so that the engine output power can be improved.

摘要翻译： 一种用于控制内燃机中的点火正时的系统和方法，其通过将点火正时校正为相位角来控制点火正时，以在爆震水平超过预定角度时避免爆震的发生，和/或通过校正 基于燃烧室压力达到其最大值的曲轴角度的校正值来确定点火正时（MBT控制）。 当发动机不处于暂态运行状态时，系统将起爆回避控制期间的点火正时的校正值或在MBT控制期间存储为对应于发动机运转状态的学习值，并基于 由发动机工作条件确定的学习值和基本点火正时。 当发动机处于瞬态运行状态时，不执行MBT控制。 因此，当发动机运转状态发生变化时，点火正时的修正值发生很大变化，不能实现转矩变动，不能进行不准确的点火正时控制，所以出现这种不良状态。 此外，由于通过过去的敲击避免控制的延迟角校正的历史反映了当前点火正时角度值的计算，因此实现了敲击发生的预防性控制。 因此，延长了MBT控制有效的驱动范围，能够提高发动机的输出功率。

公开(公告)号：US4493302A

公开(公告)日：1985-01-15

申请号：US454024

申请日：1982-12-28

申请人： Yoshihisa Kawamura

发明人： Yoshihisa Kawamura

IPC分类号： F02B1/04 ,  F02B3/06 ,  F02D41/40 ,  F02M59/20

CPC分类号： F02D41/401 ,  F02B1/04 ,  F02B3/06 ,  F02D2200/0602 ,  F02D2200/063 ,  F02D41/408 ,  Y02T10/44

摘要： A fuel injection timing control system for an internal combustion engine into which fuel is intermittently injected, includes a closed-loop fuel injection timing control system, a first device, fluidly-driven, for varying the fuel injection timing, and a second device for adjustably determining the response time and the stability of the closed-loop control system. The first device constitutes a part of the closed-loop control system. The second device constitutes another part of the closed-loop control system. Sensed is a condition of the working fluid for the first device which can influence the response time and the stability of the closed-loop control system. The second device is adjusted in accordance with the sensed working fluid condition so as to reduce the influence of the working fluid condition on the response time and the stability of the closed-loop control system.

摘要翻译： 一种用于内燃机的燃料喷射定时控制系统，其中间歇地喷射燃料，包括闭环燃料喷射正时控制系统，用于改变燃料喷射正时的流体驱动的第一装置和可调节的第二装置 确定闭环控制系统的响应时间和稳定性。 第一个装置构成闭环控制系统的一部分。 第二装置构成闭环控制系统的另一部分。 感测是第一装置的工作流体的状况，其可以影响响应时间和闭环控制系统的稳定性。 第二装置根据检测到的工作流体条件进行调整，以减少工作流体状况对响应时间和闭环控制系统的稳定性的影响。

公开(公告)号：US4471653A

公开(公告)日：1984-09-18

申请号：US461124

申请日：1983-01-26

申请人： Yoshihisa Kawamura ,  Toyoaki Nakagawa

发明人： Yoshihisa Kawamura ,  Toyoaki Nakagawa

IPC分类号： F02P17/04 ,  F02D41/34 ,  F02M65/00 ,  F02P7/077 ,  G01M15/00

CPC分类号： F02D41/009 ,  F02P7/0775 ,  F02D41/0097

摘要： A device for determining a crank shaft angular position performs operation for determining the crank shaft angular position at an occurrence of timing control in an internal combustion engine control, such as fuel injection timing control, spark ignition timing control and so forth. In determination of the crank shaft angular position, an angular acceleration is firstly calculated with respect to respective intervals of crank reference angle signals. In calculation, the determined angular acceleration is regarded as constant instead of an angular velocity. At the same time, time intervals between respectively adjacent crank reference signals and between one of crank reference signal and a timing control signal immediately following to the one of crank reference angle signal are measured. Based on the determined angular acceleration and the time interval between the one of crank reference angle signal and the timing control signal, the crank shaft angular position at the occurrence of the timing control signal is determined. This may provide determination of the crank shaft angular position precisely following to the actual crank shaft angular position even when the engine revolutional fluctuation is significant.

摘要翻译： 用于确定曲柄轴角位置的装置执行在内燃机控制中的定时控制发生时确定曲柄轴角位置的操作，例如燃料喷射正时控制，火花点火正时控制等。 在确定曲柄轴角度位置时，首先根据曲柄参考角度信号的各个间隔计算角加速度。 在计算中，所确定的角加速度被认为是恒定的而不是角速度。 同时，测量相应的曲柄参考信号之间和曲柄参考信号之一之间的时间间隔和紧跟在曲柄参考角度信号之后的定时控制信号。 基于确定的角加速度和曲柄参考角度信号与定时控制信号之间的时间间隔，确定出现定时控制信号时的曲轴角位置。 即使当发动机转动波动很大时，也可以精确地跟随实际的曲柄轴角度位置来确定曲轴角度位置。

公开(公告)号：US4466416A

公开(公告)日：1984-08-21

申请号：US404912

申请日：1982-08-03

申请人： Yoshihisa Kawamura

发明人： Yoshihisa Kawamura

IPC分类号： F02M25/07 ,  F02B3/06 ,  F02D9/02 ,  F02D11/08 ,  F02D21/08 ,  F02M25/06

CPC分类号： F02D41/005 ,  F02D11/08 ,  F02D9/02 ,  F02M26/57 ,  F02M26/64 ,  F02B3/06 ,  F02D2009/0276 ,  F02D2041/0017 ,  F02D2041/0022 ,  Y02T10/47

摘要： An exhaust gas recirculation method and apparatus controls the duty cycles of a throttle valve provided in an air intake passageway leading to a diesel engine, and an exhaust gas recirculation valve provided in an exhaust gas recirculation passageway connecting the air intake passageway and an exhaust gas passage, according to a predetermined relationship. The respective valves are controlled via corresponding diaphragm actuators and electromagnetic valves for actuating the actuators by a control unit which produces duty cycle control signals for the respective electromagnetic valves in accordance with sensed operating parameter signals which represent engine speed, load and coolant temperature. The control of the throttle and EGR valves are related so that the throttle valve is held fully open when the EGR valve is not fully open. This ensures the absolute minimum emission of exhaust HC throughout the range of throttle-EGR control.

摘要翻译： 排气再循环方法和装置控制设置在通向柴油发动机的进气通道中的节流阀的占空比，以及设置在排气再循环通道中的排气再循环阀，该废气再循环阀连接进气通道和排气通道 ，根据预定的关系。 相应的阀门通过相应的隔膜致动器和电磁阀来控制，该电磁阀通过控制单元来驱动致动器，该控制单元根据感测到的表示发动机转速，负载和冷却剂温度的操作参数信号，产生各个电磁阀的占空比控制信号。 节流阀和EGR阀的控制相关联，使得当EGR阀未完全打开时节流阀保持完全打开。 这确保了在节气门 - EGR控制范围内排气HC的绝对最小排放。