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1.发明授权
公开(公告)号：US5981183A
公开(公告)日：1999-11-09
申请号：US821782
申请日：1997-03-20
CPC分类号： C12Q1/6865
摘要： In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.
摘要翻译： 在扩增特异性核酸序列的方法中，可以进行非特异性杂交的可能性低的高度特异性扩增。 还提供了稳定的试剂，其供应和储存的活性不降低。 热稳定酶用作基于复制RNA的扩增系统所需的RNA依赖性DNA聚合酶，DNA依赖性DNA聚合酶，DNA依赖性RNA聚合酶和核糖核酸酶H。 特别优选共同使用具有RNA依赖性DNA聚合酶活性，DNA依赖性DNA聚合酶活性和核糖核酸酶H活性的嗜热栖热菌的热稳定性酶和耐热性DNA依赖型RNA聚合酶。 通过这种方法，通过使用热稳定酶来防止酶的失活，并且可以不依次加入酶进行扩增。
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2.发明授权公开(公告)号：US06303306B1
公开(公告)日：2001-10-16
申请号：US09292435
申请日：1999-04-15
IPC分类号： C12Q168
CPC分类号： C12Q1/6865 , C12Q2527/101
摘要： In a method for amplifying the specific nucleic acid sequence, a highly specific amplification which has low possibility of non-specific hybridization can be carried out. Highly stable reagents, the activities of which do not decrease in case of supply and storage, are also provided. Thermostable enzymes are used as RNA dependent DNA polymerase, DNA dependent DNA polymerase, DNA dependent RNA polymerase and ribonuclease H, which are required for the amplification system based on replicated RNA. Especially, it is preferable that a thermostable enzyme derived from Thermus thermophilus which has RNA dependent DNA polymerase activity, DNA dependent DNA polymerase activity and ribonuclease H activity, and thermostable DNA dependent RNA polymerase are used together. By this method, inactivation of enzymes are prevented by using thermostable enzymes, and the amplification can be carried out without sequential addition of enzymes.
摘要翻译： 在扩增特异性核酸序列的方法中，可以进行非特异性杂交的可能性低的高度特异性扩增。 还提供了稳定的试剂，其供应和储存的活性不降低。 热稳定酶用作基于复制RNA的扩增系统所需的RNA依赖性DNA聚合酶，DNA依赖性DNA聚合酶，DNA依赖性RNA聚合酶和核糖核酸酶H。 特别优选共同使用具有RNA依赖性DNA聚合酶活性，DNA依赖性DNA聚合酶活性和核糖核酸酶H活性的嗜热栖热菌的热稳定性酶和耐热性DNA依赖型RNA聚合酶。 通过这种方法，通过使用热稳定酶来防止酶的失活，并且可以不依次加入酶进行扩增。
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3.发明授权
公开(公告)号：US5525462A
公开(公告)日：1996-06-11
申请号：US875758
申请日：1992-04-28
申请人： Yutaka Takarada , Toshiya Aono , Shuji Shibata
发明人： Yutaka Takarada , Toshiya Aono , Shuji Shibata
CPC分类号： C12Q1/6853
摘要： An oligonucleotide is provided, which has at least a base sequence A, which is 10-30 nucleotides in length and homologous to a part of a specific nucleic acid sequence to be amplified, and a base sequence B, which is 10-30 nucleotides in length and complementary to a sequence 3' to the part of the specific nucleic acid sequence to be amplified. Sequences A and B are separated by a spacer region of 0-20 nucleotides. The oligonucleotide is used in an amplification method, wherein the oligonucleotide is mixed with a sample containing a specific nucleic acid sequence to be amplified and allowed to anneal to the specific nucleic acid sequence in the sample. The annealed oligonucleotide acts as a primer in an elongation reaction, whereas any nonannealed oligonucleotide is decomposed, except for at least part of the base sequence A. The elongation product is then denatured to a single strand for use as a template to which the remaining oligonucleotide anneals to form a primer for subsequent elongation. Also provided is a detection method, which allows for detection of a specific nucleic acid sequence in a sample, as well as reagent kits for use in the amplification and detection methods.
摘要翻译： 提供了寡核苷酸，其至少具有碱基序列A，其长度为10-30个核苷酸并且与待扩增的特异性核酸序列的一部分同源;以及碱基序列B，其为10-30个核苷酸 长度并与要扩增的特定核酸序列部分的序列3'互补。 序列A和B被0-20个核苷酸的间隔区隔开。 寡核苷酸用于扩增方法，其中将寡核苷酸与含有待扩增的特异性核酸序列的样品混合并使其与样品中的特定核酸序列退火。 退火的寡核苷酸在伸长反应中用作引物，而除了至少部分碱基序列A之外，任何非退火的寡核苷酸都被分解。然后将延伸产物变性为单链，用作剩余的寡核苷酸 退火以形成随后伸长的底漆。 还提供了一种检测方法，其允许检测样品中的特定核酸序列，以及用于扩增和检测方法的试剂盒。
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公开(公告)号：US20050168192A1
公开(公告)日：2005-08-04
申请号：US11044563
申请日：2005-01-28
申请人： Kazuhisa Nagase , Shin Suzuki , Shuji Shibata , Shingo Takahashi
发明人： Kazuhisa Nagase , Shin Suzuki , Shuji Shibata , Shingo Takahashi
CPC分类号： H02J7/0047 , H02J2007/005 , H02J2007/0098
摘要： The invention provides a battery pack 2 operatively associated with a main system 1 for charge-level data sharing between them for recording and management. The battery pack 2 includes recorder means 22 that is connected to the main system 1 for writing to and reading out of the main system 1. Charge-level data measured (12, 14) and calculated (13) at at least the main system 1 are written to and read out of the recorder means 22 by way of signal lines. The battery pack 2 is not provided with any means for management of the charge-level data, making cost and size reductions possible.
摘要翻译： 本发明提供了一种与主系统1可操作地相关联的电池组2，用于在它们之间进行电荷级数据共享用于记录和管理。 电池组2包括连接到主系统1用于写入和读出主系统1的记录器装置22。 至少在主系统1处测量的电荷电平数据（12,14）和计算出的（13）通过信号线被写入和读出记录器装置22。 电池组2没有提供用于管理电荷水平数据的任何装置，从而可以降低成本和尺寸。
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5.发明申请Substrate processing apparatus, and combined system of functional blocks for use in substrate processing apparatus 审中-公开基板处理装置和用于基板处理装置的功能块的组合系统公开(公告)号：US20050061247A1
公开(公告)日：2005-03-24
申请号：US10946954
申请日：2004-09-22
申请人： Shuji Shibata , Yoshihisa Yamada , Masayoshi Shiga
发明人： Shuji Shibata , Yoshihisa Yamada , Masayoshi Shiga
IPC分类号： B05D3/00 , H01L21/00 , H01L21/027 , H01L21/677 , C23C16/00
CPC分类号： H01L21/67173 , H01L21/67178 , H01L21/67184
摘要： A substrate processing apparatus is mechanically divided into processing blocks including an indexer block 1, a BARC block 2, a resist coating block 3, a development block 4, an interface block 5 and an inspection block IB. These processing blocks each comprise a transport robot and a substrate holding part which together serve as a substrate transport mechanism. The substrate holding parts of the processing blocks all have the same structure. The transport robot in each of the processing blocks is accessible to the substrate holding part belonging to an adjacent block.
摘要翻译： 基板处理装置被机械地划分为包括索引器块1，BARC块2，抗蚀剂涂层块3，显影块4，界面块5和检查块IB的处理块。 这些处理块各自包括一起用作基板输送机构的输送机器人和基板保持部。 处理块的基板保持部分都具有相同的结构。 每个处理块中的传送机器人可访问属于相邻块的基板保持部。
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公开(公告)号：US07497633B2
公开(公告)日：2009-03-03
申请号：US11273440
申请日：2005-11-10
申请人： Koji Kaneyama , Shuji Shibata , Tsuyoshi Okumura , Shuichi Yasuda , Masashi Kanaoka , Tadashi Miyagi , Kazuhito Shigemori
发明人： Koji Kaneyama , Shuji Shibata , Tsuyoshi Okumura , Shuichi Yasuda , Masashi Kanaoka , Tadashi Miyagi , Kazuhito Shigemori
CPC分类号： H01L21/67051 , G03F7/70341 , G03F7/70991 , H01L21/67028 , H01L21/67034 , Y10S414/135
摘要： A substrate processing apparatus comprises an indexer block, an anti-reflection film processing block, a resist film processing block, a drying/development processing block, and an interface block. An exposure device is arranged adjacent to the interface block. The drying/development processing block comprises a drying processing group. The interface block comprises an interface transport mechanism. A substrate is subjected to exposure processing by the exposure device, and subsequently transported to the drying processing group by the interface transport mechanism. The substrate is cleaned and dried by the drying processing group.
摘要翻译： 基板处理装置包括分度器块，防反射膜处理块，抗蚀剂膜处理块，干燥/显影处理块和界面块。 曝光装置设置在接口块附近。 干燥/显影处理块包括干燥处理组。 接口块包括接口传送机制。 基板经曝光装置进行曝光处理，随后通过界面输送机构输送到干燥处理组。 通过干燥处理组对基材进行清洗干燥。
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公开(公告)号：US20060108068A1
公开(公告)日：2006-05-25
申请号：US11273440
申请日：2005-11-10
申请人： Koji Kaneyama , Shuji Shibata , Tsuyoshi Okumura , Shuichi Yasuda , Masashi Kanaoka , Tadashi Miyagi , Kazuhito Shigemori
发明人： Koji Kaneyama , Shuji Shibata , Tsuyoshi Okumura , Shuichi Yasuda , Masashi Kanaoka , Tadashi Miyagi , Kazuhito Shigemori
IPC分类号： C23F1/00 , B08B7/00 , C23C16/00 , H01L21/677
CPC分类号： H01L21/67051 , G03F7/70341 , G03F7/70991 , H01L21/67028 , H01L21/67034 , Y10S414/135
摘要： A substrate processing apparatus comprises an indexer block, an anti-reflection film processing block, a resist film processing block, a drying/development processing block, and an interface block. An exposure device is arranged adjacent to the interface block. The drying/development processing block comprises a drying processing group. The interface block comprises an interface transport mechanism. A substrate is subjected to exposure processing by the exposure device, and subsequently transported to the drying processing group by the interface transport mechanism. The substrate is cleaned and dried by the drying processing group.
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