公开(公告)号：US20200248116A1

公开(公告)日：2020-08-06

申请号：US16757133

申请日：2018-10-19

Applicant: President and Fellows of Harvard College

Inventor： Benjamin D. Pope ,  Kevin Kit Parker

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/077 ,  A61K35/28

Abstract: The present invention provides methods and systems which accommodate 3-dimensional adipocyte expansion to produce, e.g., mature adipocytes and synthetic adipose tissue with cellular properties of mature adult organisms, including cell size and cytoarchitecture, and the use of such methods and systems for, e.g., in vitro drug screening and/or toxicity assays, disease modeling, and therapeutic applications.

公开(公告)号：US10591458B2

公开(公告)日：2020-03-17

申请号：US15116258

申请日：2015-02-18

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Johan Ulrik Lind ,  Jennifer Ann Lewis ,  Joost Johan Vlassak ,  Hongyan Yuan ,  Travis Alexander Busbee ,  Ian Perkins ,  Christophe Chantre

IPC: G01N33/483 ,  A61B5/00 ,  A61B5/053

Abstract: Embodiments described herein are directed to devices for supporting growth of anisotropic muscle tissue layers and in vitro readout and quantification of force generated by the tissue layers using one or more strain-sensing elements integrated into the device. Embodiments also include multiplexed apparatuses of multiple independent devices, methods of fabricating the devices and apparatuses, and methods of using the devices and apparatuses.

公开(公告)号：US20190032021A1

公开(公告)日：2019-01-31

申请号：US16125433

申请日：2018-09-07

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Kevin Kit Parker ,  Geraldine A. Hamilton ,  Anthony Bahinski

IPC: C12N5/071 ,  C12M1/42 ,  C12M3/06 ,  C12M1/12 ,  C12M1/00

Abstract: Disclosed herein are organ chips that can be individually used or integrated together to form different microphysiological systems, e.g., for use in cell culturing, drug screening, toxicity assays, personalized therapeutic treatment, scaffolding in tissue repair and/or replacement, and/or pharmacokinetic or pharmacodynamics studies.

公开(公告)号：US20180372725A1

公开(公告)日：2018-12-27

申请号：US15869228

申请日：2018-01-12

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Mohammad Reza Badrossamay ,  Ashutosh Agarwal

IPC: G01N33/50 ,  C12N5/071 ,  C12N5/077

Abstract: The present invention provides devices, constructs, and methods of use of polymeric fiber-scaffolded engineered tissues and assays for identifying compounds that modulate a contractile function, using such devices and constructs.

公开(公告)号：US20180172672A1

公开(公告)日：2018-06-21

申请号：US15662371

申请日：2017-07-28

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Adam W. Feinberg ,  Patrick W. Alford ,  Anna Grosberg ,  Mark Daniel Brigham ,  Josue A. Goss

IPC: G01N33/50 ,  C12Q1/02 ,  C40B30/06

CPC classification number: G01N33/5061 ,  C12Q1/025 ,  C40B30/06 ,  G01N33/5032 ,  G01N33/5088 ,  G01N2500/10

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for use in these assays.

公开(公告)号：US09669141B2

公开(公告)日：2017-06-06

申请号：US14415945

申请日：2013-07-19

Applicant: President and Fellows of Harvard College ,  California Institute of Technology

Inventor： Kevin Kit Parker ,  Josue A. Goss ,  Sung-Jin Park ,  Andrew K. Capulli ,  Holly M. Golecki ,  Janna C. Nawroth ,  John O. Dabiri

IPC: A61M1/10 ,  A61F2/24 ,  G01N33/50 ,  A61M1/12

CPC classification number: A61M1/1003 ,  A61F2/2415 ,  A61M1/1037 ,  A61M1/1053 ,  A61M1/1098 ,  A61M1/12 ,  A61M1/122 ,  A61M1/127 ,  G01N33/5061 ,  G01N2500/04 ,  G01N2500/10 ,  Y10T29/49405

Abstract: The present invention provides tissue-engineered pumps and valves, methods of fabricating such pumps and valves, and methods of use of such pumps and valves.

公开(公告)号：US20160290903A1

公开(公告)日：2016-10-06

申请号：US15034901

申请日：2014-11-07

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Leila F. Deravi ,  Borna E. Dabiri

IPC: G01N3/08 ,  C08L89/00 ,  C08L25/18 ,  C07K14/78

CPC classification number: G01N3/08 ,  C07K14/78 ,  C08L25/18 ,  C08L89/00

Abstract: The present invention provides in vitro models of non-penetrating traumatic brain injury, which include a network of extracellular matrix (ECM) protein fibrils, methods for preparing such models, and uses of such models for, e.g., identifying compounds suitable for preventing or treating non-penetrating traumatic brain injury.

Abstract translation: 本发明提供非穿透性创伤性脑损伤的体外模型，其包括细胞外基质（ECM）蛋白原纤维网络，制备这种模型的方法，以及这些模型用于鉴定适合于预防或治疗的化合物 非穿透性创伤性脑损伤。

公开(公告)号：US09383350B2

公开(公告)日：2016-07-05

申请号：US13922432

申请日：2013-06-20

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Adam W. Feinberg ,  George M. Whitesides ,  Sergey S. Shevkoplyas ,  Alexander Feigel

IPC: G01N33/50 ,  C12N5/00 ,  C12N5/077

CPC classification number: G01N33/5061 ,  C12N5/0062 ,  C12N5/0657 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2539/10 ,  G01N2500/10

Abstract: A free-standing thin film is fabricated from a structure comprising a base layer coated with a sacrificial polymer layer, which is in turn coated with a flexible polymer layer. Cells are then seeded onto the flexible polymer layer and cultured to form a tissue. The flexible polymer layer is then released from the base layer to produce a free-standing thin film comprising the tissue on the flexible polymer layer. In one embodiment, the cells are myocytes, which can be actuated to propel or displace the free-standing film. In another embodiment, the free-standing film is used to treat injured human tissue.

Abstract translation: 独立的薄膜由包括涂覆有牺牲聚合物层的基层的结构制成，牺牲聚合物层又被柔性聚合物层涂覆。 然后将细胞接种到柔性聚合物层上并进行培养以形成组织。 然后将柔性聚合物层从基底层释放出来，以产生包含柔性聚合物层上的组织的自立薄膜。 在一个实施方案中，细胞是肌细胞，其可被致动以推动或置换自立膜。 在另一个实施方案中，自立膜用于治疗受损的人体组织。

公开(公告)号：US20140342394A1

公开(公告)日：2014-11-20

申请号：US14362287

申请日：2012-12-10

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Josue Adrian Goss ,  Anna Grosberg ,  Patrick W. Alford ,  Adam W. Feinberg ,  Ashutosh Agrawal ,  Megan Laura McCain ,  Johan Ulrik Lind

IPC: G01N33/50

CPC classification number: G01N33/5008 ,  G01N33/5088 ,  G01N2500/04 ,  G01N2500/10

Abstract: The present invention provides high throughput assays for identifying compounds that modulate a contractile function, as well as devices suitable for these assays.

Abstract translation: 本发明提供用于鉴定调节收缩功能的化合物的高通量测定，以及适用于这些测定的装置。

公开(公告)号：US20140322515A1

公开(公告)日：2014-10-30

申请号：US14359005

申请日：2012-11-16

Applicant: President and Fellows of Harvard College

Inventor： Kevin Kit Parker ,  Mohammad Reza Badrossamay ,  Josue Adrian Goss

IPC: B29D99/00 ,  D01F6/62

CPC classification number: B29D99/0078 ,  D01D5/00 ,  D01D5/0985 ,  D01D5/18 ,  D01F6/625 ,  Y10T428/249921

Abstract: In accordance with an exemplary embodiment, a method is provided for forming a micron, submicron and/or nanometer dimension polymeric fiber. The method includes providing a stationary deposit of a polymer. The method also includes contacting a surface of the polymer to impart sufficient force in order to decouple a portion of the polymer from the contact and to fling the portion of the polymer away from the contact and from the deposit of the polymer, thereby forming a micron, submicron and/or nano-meter dimension polymeric fiber.

Abstract translation: 根据示例性实施例，提供了一种用于形成微米，亚微米和/或纳米尺寸聚合物纤维的方法。 该方法包括提供聚合物的固定沉积物。 该方法还包括使聚合物的表面接触以赋予足够的力以使聚合物的一部分与接触体分离，并使聚合物的部分远离接触和聚合物的沉积，从而形成微米 ，亚微米和/或纳米尺寸聚合物纤维。