公开(公告)号：US11312949B2

公开(公告)日：2022-04-26

申请号：US16661595

申请日：2019-10-23

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Alexander L. Watters ,  Philip T. Snell ,  Donald E. Ingber

IPC: C12N9/96 ,  C12N9/26 ,  A61K38/00 ,  C07K14/805

Abstract: Described herein are heme-binding compositions and methods relating to their use, for example methods of treatment of sepsis and rhabdomyolysis.

公开(公告)号：US11119093B2

公开(公告)日：2021-09-14

申请号：US15105962

申请日：2014-12-19

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Geraldine A. Hamilton ,  Bryan Hassell ,  Christopher D. Hinojosa ,  Carolina Lucchesi

IPC: G01N33/50 ,  C12M3/06 ,  C12M1/12 ,  C12M1/42 ,  C12M3/00 ,  C12M1/34

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

公开(公告)号：US11059873B2

公开(公告)日：2021-07-13

申请号：US16702724

申请日：2019-12-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Jeffrey Charles Way ,  Donald E. Ingber

IPC: C07K14/47 ,  G01N33/569 ,  C07K16/44 ,  C07K17/00 ,  G01N33/50 ,  G01N27/74

Abstract: The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.

公开(公告)号：US20210170007A1

公开(公告)日：2021-06-10

申请号：US17015177

申请日：2020-09-09

Applicant: President and Fellows of Harvard College

Inventor： Michael Super ,  Edward J. Doherty ,  Mark Joseph Cartwright ,  Des White ,  Alexander Stafford ,  Omar Abdel-Rahman Ali ,  Amanda Graveline ,  Donald E. Ingber ,  David J. Mooney ,  Benjamin Seiler

IPC: A61K39/108 ,  A61K39/02

Abstract: The present invention provides vaccine compositions and methods of producing such compositions. Other embodiments of the invention include methods of treating a pathogen infection, methods of vaccinating a subject against a pathogen infection, and methods for treating an antibiotic-resistance bacterial infection in a subject in need thereof. In further embodiments, the invention includes methods of decreasing the level of a pathogen in a subject having a pathogen infection, methods of increasing the surviving rate of a subject having a pathogen infection, methods of reducing the level of pain associated with a pathogen infection, and methods of reducing the level of distress associated with a pathogen infection in a subject in need thereof. Novel scaffold compositions and opsonin-bound or lectin-bound pathogen compositions, and uses thereof, are also provided herein.

公开(公告)号：US10813988B2

公开(公告)日：2020-10-27

申请号：US15434781

申请日：2017-02-16

Applicant: President and Fellows of Harvard College

Inventor： Michael Super ,  Edward J. Doherty ,  Mark Cartwright ,  Des White ,  Alexander G. Stafford ,  Omar Abdel-Rahman Ali ,  Amanda Graveline ,  Donald E. Ingber ,  David J. Mooney ,  Benjamin Seiler

IPC: A61K39/108 ,  A61K39/02 ,  A61K39/00

Abstract: The present invention provides vaccine compositions and methods of producing such compositions. Other embodiments of the invention include methods of treating a pathogen infection, methods of vaccinating a subject against a pathogen infection, and methods for treating an antibiotic-resistance bacterial infection in a subject in need thereof. In further embodiments, the invention includes methods of decreasing the level of a pathogen in a subject having a pathogen infection, methods of increasing the surviving rate of a subject having a pathogen infection, methods of reducing the level of pain associated with a pathogen infection, and methods of reducing the level of distress associated with a pathogen infection in a subject in need thereof. Novel scaffold compositions and opsonin-bound or lectin-bound pathogen compositions, and uses thereof, are also provided herein.

公开(公告)号：US20200292531A1

公开(公告)日：2020-09-17

申请号：US15929387

申请日：2020-04-29

Applicant: President and Fellows of Harvard College ,  The Children's Medical Center Corporation

Inventor： Donald E. Ingber ,  Abhishek Jain ,  Andries D. van der Meer ,  Alan David Michelson ,  Andrew L. Frelinger, III ,  Riccardo Barrile

IPC: G01N33/50 ,  B01L3/00 ,  G01N33/86

Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.

公开(公告)号：US10407655B2

公开(公告)日：2019-09-10

申请号：US15403944

申请日：2017-01-11

Applicant: President and Fellows of Harvard College

Inventor： Christopher David Hinojosa ,  Josiah Sliz ,  Daniel Levner ,  Guy Thompson ,  Hubert Geisler ,  Jose Fernandez-Alcon ,  Donald E. Ingber

IPC: B01L3/00 ,  C12M1/34 ,  C12M1/26 ,  C12M3/00 ,  C12M3/06 ,  B82Y15/00 ,  G01N33/50

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

公开(公告)号：US20190106665A1

公开(公告)日：2019-04-11

申请号：US16134746

申请日：2018-09-18

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Anthony Bahinski ,  Robert Cunningham ,  Josue A. Goss ,  Geraldine A. Hamilton ,  Christopher David Hinojosa ,  Daniel Levner ,  Kevin Kit Parker

IPC: C12M3/06 ,  G01N33/50 ,  B01L3/00 ,  B01L9/00 ,  C12M1/42 ,  C12M1/00 ,  A01N1/02 ,  C12M1/34 ,  C12M3/00

Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

公开(公告)号：US09988617B2

公开(公告)日：2018-06-05

申请号：US14892252

申请日：2014-05-21

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Michael Super ,  Alexander L. Watters ,  Philip T. Snell ,  Donald E. Ingber

IPC: C12N9/96 ,  C12N9/26 ,  A61K38/00 ,  C07K14/805

CPC classification number: C12N9/2474 ,  A61K38/00 ,  C07K14/805 ,  C12N9/96 ,  C12Y302/01035

Abstract: Described herein are heme-binding compositions and methods relating to their use, e.g. methods of treatment of, for example, sepsis and rhabdomyolysis.

公开(公告)号：US09855554B2

公开(公告)日：2018-01-02

申请号：US14906335

申请日：2014-07-22

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Christopher David Hinojosa

IPC: B01L3/00 ,  B01L99/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/56 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655

Abstract: According to aspects of the present invention, a cartridge assembly for transporting fluid into or out of one or more fluidic devices includes a first layer and a second layer. The first layer includes a first surface. The first surface includes at least one partial channel disposed thereon. The second layer abuts the first surface, thereby forming a channel from the at least one partial channel. At least one of the first layer and the second layer is a resilient layer formed from a pliable material. At least one of the first layer and the second layer includes a via hole. The via hole is aligned with the channel to pass fluid thereto. The via hole is configured to pass fluid through the first layer or the second layer substantially perpendicularly to the channel. Embossments are also used to define aspects of a fluidic channel.