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公开(公告)号:US10570446B2
公开(公告)日:2020-02-25
申请号:US16200540
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US10457984B2
公开(公告)日:2019-10-29
申请号:US16150180
申请日:2018-10-02
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C12Q1/68 , C07H19/14 , C12Q1/6869 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US10407458B2
公开(公告)日:2019-09-10
申请号:US16149098
申请日:2018-10-01
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/68 , C07H21/00 , C12Q1/686 , C12Q1/6874 , C12Q1/6872 , C12Q1/6869 , C07H19/10 , C12Q1/6876 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190135851A1
公开(公告)日:2019-05-09
申请号:US16200571
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/6874 , C12Q1/6876 , C07H21/00 , C12Q1/686 , C12Q1/6872 , C12Q1/6869 , C07H19/10 , C12Q1/68
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190135850A1
公开(公告)日:2019-05-09
申请号:US16200540
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/6874 , C12Q1/6876 , C07H21/00 , C12Q1/686 , C12Q1/6872 , C12Q1/6869 , C07H19/10 , C12Q1/68
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190092806A1
公开(公告)日:2019-03-28
申请号:US16200564
申请日:2018-11-26
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190085015A1
公开(公告)日:2019-03-21
申请号:US16150180
申请日:2018-10-02
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/6872 , C12Q1/6876 , C07H19/10 , C12Q1/6869 , C12Q1/6874 , C12Q1/68 , C07H21/00 , C12Q1/686
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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公开(公告)号:US20190031704A1
公开(公告)日:2019-01-31
申请号:US16149098
申请日:2018-10-01
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
IPC分类号: C07H19/14 , C12Q1/6869 , C07H19/10 , C12Q1/6874 , C12Q1/6876 , C12Q1/6872 , C12Q1/68 , C12Q1/686
CPC分类号: C12Q1/6869 , C07B2200/11 , C07H19/10 , C07H19/14 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2525/186 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C12Q2565/501 , C40B40/00
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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49.
公开(公告)号:US10144961B2
公开(公告)日:2018-12-04
申请号:US15596379
申请日:2017-05-16
发明人: Jingyue Ju , Huanyan Cao , Zengmin Li , Qinglin Meng , Jia Guo , Shenglong Zhang , Lin Yu
摘要: This invention provides novel azido linkers for deoxynucleotide analogs having a detectable marker attached thereto.
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公开(公告)号:US20170088891A1
公开(公告)日:2017-03-30
申请号:US15380311
申请日:2016-12-15
发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
CPC分类号: C07H19/14 , C07B2200/11 , C07H19/10 , C07H21/00 , C12Q1/68 , C12Q1/686 , C12Q1/6869 , C12Q1/6872 , C12Q1/6874 , C12Q1/6876 , C12Q2525/117 , C12Q2535/101 , C12Q2535/122 , C12Q2563/107 , C40B40/00 , C12Q2565/501 , C12Q2525/186
摘要: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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