公开(公告)号：US11061003B2

公开(公告)日：2021-07-13

申请号：US16601860

申请日：2019-10-15

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Keith Fadgen ,  Peyton C. Beals ,  Sylvain Gilles Cormier ,  Richard Wayne Andrews ,  Geoff C. Gerhardt ,  Jordan Kornfeld ,  Thomas S. McDonald

IPC: G01N30/06 ,  G01N30/20

Abstract: Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample.

公开(公告)号：US20210156826A1

公开(公告)日：2021-05-27

申请号：US17095280

申请日：2020-11-11

Applicant: Waters Technologies Corporation

Inventor： Jason F. Hill ,  Sylvain Gilles Cormier ,  Martin Gilar

IPC: G01N30/20 ,  G01N30/10 ,  G01N30/34

Abstract: Described herein are a selectable flow restriction device and a fluidic network for chromatographic sample dilution during injection. A selectable flow restriction device is used in combination with a splitter to define an adjustable splitter for separating a high-pressure solvent flow into two separate solvent flows having the same solvent composition. The first solvent flow passes to a sample manager where a sample is injected as a discrete fluidic plug at an injection valve into a flow comprising at least one solvent. The second solvent flow is merged downstream from the location of injection with the first solvent carrying the sample fluidic plug. The second solvent flow dilutes the sample plug during the mixture of the two solvent flows.

公开(公告)号：US20200271629A1

公开(公告)日：2020-08-27

申请号：US16800454

申请日：2020-02-25

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Martin Gilar ,  Mathew H. DeLano ,  Michael Donegan

IPC: G01N30/26 ,  B01D15/22 ,  B01D15/38 ,  B01D15/36 ,  B01D15/18

Abstract: The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. The method includes introducing a sample comprising the analytes into a chromatographic system. The chromatographic system has a flow path disposed in an interior of the chromatographic system, at least a portion of the flow path having an active coating, and a chromatographic column having a stationary phase material in an interior of the chromatographic column that facilitates separation of the analytes in the sample through interaction with at least one analyte in the sample. The active coating is selected to interact with at least one analyte in the sample through (1) a repulsive force, (2) a secondary interaction, or (3) a retention mechanism distinct from the interaction with the stationary phase material.

公开(公告)号：US10705060B2

公开(公告)日：2020-07-07

申请号：US15553268

申请日：2016-02-25

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Joseph D. Michienzi ,  Martin Gilar ,  Abhijit Tarafder

IPC: G01N30/30 ,  G01N30/34 ,  G01N30/86 ,  B01D15/16 ,  G01N30/46 ,  G01N30/02

Abstract: Methods for focusing analyte peaks in liquid chromatography using a spatial temperature gradient are provided. Also provided are methods for focusing analyte peaks and improving resolution using a trap column upstream of a separation column. Further, methods are provided in which the trap column placed upstream of the separation column is packed with a temperature-sensitive polymer/copolymer, and a spatial temperature gradient is applied along the trap column for obtaining improved retentivity by trap column stationary phase, and overall improved resolution of analyte peaks.

公开(公告)号：US20200041463A1

公开(公告)日：2020-02-06

申请号：US16601860

申请日：2019-10-15

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Keith Fadgen ,  Peyton C. Beals ,  Sylvain Gilles Cormier ,  Richard Wayne Andrews ,  Geoff C. Gerhardt ,  Jordan Kornfeld ,  Thomas S. McDonald

IPC: G01N30/06 ,  G01N30/20

Abstract: Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample.

公开(公告)号：US20190170706A1

公开(公告)日：2019-06-06

申请号：US16098280

申请日：2017-05-04

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Richard W. Andrews ,  Peyton C. Beals ,  Thomas McDonald ,  Keith Fadgen ,  Jordan Kornfeld ,  Geoff Gerhardt ,  Sylvain Cormier

IPC: G01N30/24 ,  G01N30/06

Abstract: A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.

公开(公告)号：US20180224404A1

公开(公告)日：2018-08-09

申请号：US15891463

申请日：2018-02-08

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Martin Gilar ,  Fabrice Gritti ,  Joseph D. Michienzi

IPC: G01N30/30

CPC classification number: G01N30/30 ,  G01N30/34 ,  G01N30/6095 ,  G01N2030/027 ,  G01N2030/3015 ,  G01N2030/3038 ,  G01N2030/3046 ,  G01N2030/3053

Abstract: A method of performing a chromatographic separation includes generating a spatial temperature gradient along a length of a chromatographic column in a liquid chromatography system. A sample is injected into a flow of a mobile phase to the column and a flow of a mobile phase having a composition gradient is provided to the column after the sample is received at the column. The spatial temperature gradient is moved along the length of the column from the column inlet to the column outlet during the time that the composition gradient traverses the column. This coordination of the composition gradient with the movement of the spatial thermal gradient yields a significant increase in peak capacity per unit time compared with conventional separation techniques performed in a conventional isothermal column environment.