公开(公告)号：US06489100B1

公开(公告)日：2002-12-03

申请号：US09440503

申请日：1999-11-15

申请人： James C. Liao

发明人： James C. Liao

IPC分类号： C12Q168

CPC分类号： C12N9/1294 ,  C12N15/52 ,  C12P7/42 ,  C12P13/227

摘要： Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.

摘要翻译： 利用包含表达载体和编码磷酸烯醇丙酮酸合酶的基因的遗传元件来增强碳资源转移到共同的芳香途径和从其分支的途径。 磷酸烯醇丙酮酸合酶的过表达使DAHP产生增加到接近理论产量。

公开(公告)号：US5985617A

公开(公告)日：1999-11-16

申请号：US277183

申请日：1999-03-26

申请人： James C. Liao

发明人： James C. Liao

IPC分类号： C12N1/21 ,  C12N9/12 ,  C12N15/52 ,  C12P7/42 ,  C12P13/22 ,  C12P19/00 ,  C12N9/24 ,  C12N15/31

CPC分类号： C12N9/1294 ,  C12N15/52 ,  C12P13/227 ,  C12P7/42

摘要： Genetic elements comprising expression vectors and a gene coding for phosphoenol pyruvate synthase is utilized to enhance diversion of carbon resources into the common aromatic pathway and pathways branching therefrom. The overexpression of phosphoenol pyruvate synthase increases DAHP production to near theoretical yields.

公开(公告)号：US5256551A

公开(公告)日：1993-10-26

申请号：US731309

申请日：1991-07-17

申请人： Masato Terasawa ,  Mitsunobu Shimazu ,  Fuzio Endo ,  Hideaki Yukawa

发明人： Masato Terasawa ,  Mitsunobu Shimazu ,  Fuzio Endo ,  Hideaki Yukawa

IPC分类号： C12N1/00 ,  C12N9/99 ,  C12P13/22 ,  C12N1/04 ,  C12N1/20

CPC分类号： C12P13/227 ,  C12N1/00 ,  C12N9/99 ,  Y10S435/849

摘要： A method of selectively inhibiting pyruvic acid decomposition activity in microorganism cells containing tryptophanase or a treated product thereof, which comprises heat-treating said cells or the treated product thereof in the presence of an ammonium ion.

摘要翻译： 选择性抑制含有色氨酸酶或其处理产物的微生物细胞中的丙酮酸分解活性的方法，其包括在铵离子存在下热处理所述细胞或其处理产物。

公开(公告)号：US4761374A

公开(公告)日：1988-08-02

申请号：US871315

申请日：1986-06-06

申请人： Teruhiko Beppu ,  Seibun Suzuki

发明人： Teruhiko Beppu ,  Seibun Suzuki

IPC分类号： C12N9/88 ,  C12P13/22 ,  C12P39/00 ,  C12R1/01 ,  C12R1/07 ,  C12N1/20

CPC分类号： C12R1/07 ,  C12N9/88 ,  C12P13/227 ,  C12P39/00 ,  C12R1/01 ,  Y10S435/822 ,  Y10S435/832

摘要： A thermally stable tryptophanase having (1) an optimum temperature for activity at a pH of 8.0 of about 70.degree. C., and (2) such thermal stability that it is not thermally deactivated when maintained at temperatures up to about 65.degree. C. and a pH of 8.0 for 40 minutes. The thermally stable tryptophanase can be produced by cultivating in a tryptophan-containing culture medium a thermally stable tryptophanase-producing bacterium which does not grow alone in said medium but grows there in the presence of Bacillus sp. strain S, and obtaining the resulting thermally stable tryptophanase from the culture broth. The thermally stable tryptophanase-producing microorganism for use in the above process is a novel organism.

摘要翻译： 一种热稳定的色氨酸酶，其具有（1）在约8.0℃的pH为8.0时的活性的最适温度，和（2）这样的热稳定性，其在高至约65℃的温度下保持不热失活， pH为8.0，持续40分钟。 热稳定色氨酸酶可以通过在含色氨酸的培养基中培养产生热稳定的产生色氨酸的热稳定的色氨酸酶细菌来生产，所述细菌在所述培养基中不单独生长，而在芽孢杆菌存在下在那里生长。 菌株S，从培养液中得到热稳定的色氨酸酶。 用于上述方法的热稳定的产生色氨酸酶的微生物是新的生物体。

公开(公告)号：US4742007A

公开(公告)日：1988-05-03

申请号：US702560

申请日：1985-02-15

申请人： Kuniki Kino ,  Toshihide Nakanishi ,  Masahiro Sugimoto

发明人： Kuniki Kino ,  Toshihide Nakanishi ,  Masahiro Sugimoto

IPC分类号： C12P13/22 ,  C12R1/15 ,  C12N1/20

CPC分类号： C12R1/15 ,  C12P13/227 ,  Y10S435/843

摘要： L-tryptophan can be prepared in good yield by a fermentation process which comprises culturing a novel L-tryptophan-producing microorganism of the genus Corynebacterium, which is resistant to at least one member selected from glyphosate [N-phosphonomethyl glycine], paraquat [1,1'-dimethyl-4,4'-bispyridinium] and derivatives thereof, and recovering L-tryptophan from the culture broth.

摘要翻译： 可以通过发酵方法以良好的产率制备L-色氨酸，其包括培养棒状杆菌属的新型L-色氨酸生产微生物，其对选自草甘膦[N-膦酰基甲基甘氨酸]，百草枯[1 ，1'-二甲基-4,4'-二吡啶鎓]及其衍生物，并从培养液中回收L-色氨酸。

公开(公告)号：US4481362A

公开(公告)日：1984-11-06

申请号：US494692

申请日：1983-05-17

申请人： Mamoru Nakai ,  Tokio Ohshima ,  Tomio Kimura ,  Tetsuo Omata ,  Noritada Iwamoto

发明人： Mamoru Nakai ,  Tokio Ohshima ,  Tomio Kimura ,  Tetsuo Omata ,  Noritada Iwamoto

IPC分类号： C12N9/80 ,  C12P13/04 ,  C12P13/22 ,  C12P41/00 ,  C12R1/025 ,  C12R1/05 ,  C12R1/06 ,  C12R1/07 ,  C12R1/13 ,  C12R1/145 ,  C12R1/20 ,  C12R1/225 ,  C12R1/265 ,  C12R1/32 ,  C12R1/365 ,  C12R1/38 ,  C12R1/46 ,  C12R1/465 ,  C12R1/645 ,  C12R1/65 ,  C12R1/66 ,  C12R1/72 ,  C12R1/77 ,  C12R1/78 ,  C12R1/80 ,  C12R1/84 ,  C12R1/845 ,  C12R1/85 ,  C12R1/88 ,  C12R1/885 ,  C07D209/20

CPC分类号： C12N9/80 ,  C12P13/04 ,  C12P13/227

摘要： Biochemical optical resolution of DL-tryptophanes in which DL-tryptophane amides are interacted with the culture products, or their treated products, of a microorganism capable of producing amidase is described. L-tryptophane amides in racemic DL-tryptophane amides are asymmetrically hydrolyzed to form optically active L-tryptophanes at a high yield and almost all D-tryptophane amides remain without being subjected to hydrolysis. The resultant D-tryptophane amides are readily hydrolyzed, after separating L-tryptophanes, to form optically active D-tryptophanes at a high yield.

摘要翻译： 描述DL-色氨酰胺与能够产生酰胺酶的微生物或其处理产物相互作用的DL-色氨酸的生物化学光学拆分。 外消旋的DL-色氨酰胺中的L-色氨酰胺不对称地水解，以高产率形成光学活性的L-色氨酸，并且几乎所有的D-色氨酸酰胺保持不被水解。 得到的D-色氨酰胺在分离L-色氨酸后容易水解，以高产率形成光学活性的D-色氨酸。

公开(公告)号：US4349627A

公开(公告)日：1982-09-14

申请号：US274566

申请日：1981-06-17

申请人： Akio Mimura ,  Yasuyuki Takahashi ,  Katsumi Yuasa ,  Mitsuru Shibukawa

发明人： Akio Mimura ,  Yasuyuki Takahashi ,  Katsumi Yuasa ,  Mitsuru Shibukawa

IPC分类号： C12P13/22 ,  C12R1/01 ,  C12R1/22 ,  C12N1/20

CPC分类号： C12R1/22 ,  C12P13/227 ,  C12R1/01 ,  Y10S435/822

摘要： A process for producing L-tryptophan or a derivative thereof is disclosed, wherein an indole compound is reacted with serine, or with pyruvic acid and/or its salt and ammonium ion, in the presence of a culture or treated culture of a particular microorganism of genus Enterobacter having a special ability to produce L-tryptophan or a derivative thereof from an indole compound and serine, or from an indole compound, pyruvic acid and/or its salt, and ammonium ion.

摘要翻译： 公开了一种生产L-色氨酸或其衍生物的方法，其中吲哚化合物与丝氨酸反应，或与丙酮酸和/或其盐和铵离子反应，在培养物或经处理的培养物存在下， 具有从吲哚化合物和丝氨酸或从吲哚化合物，丙酮酸和/或其盐和铵离子产生L-色氨酸或其衍生物的特殊能力的肠杆菌属。

公开(公告)号：US4271267A

公开(公告)日：1981-06-02

申请号：US101041

申请日：1979-12-06

申请人： Hideaki Yukawa ,  Kazuoki Osumi ,  Terukatsu Nara ,  Yoshihiro Takayama

发明人： Hideaki Yukawa ,  Kazuoki Osumi ,  Terukatsu Nara ,  Yoshihiro Takayama

IPC分类号： C12P13/22 ,  C12R1/425

CPC分类号： C12P13/227 ,  Y10S435/881

摘要： A process for preparing L-tryptophan by fermentation which comprises cultivating on a medium containing ethanol as the main carbon source a microorganism of the genus Serratia that utilizes ethanol and has the ability to produce L-tryptophan, and recovering the accumulated L-tryptophan from the culture.

摘要翻译： 一种通过发酵制备L-色氨酸的方法，其包括在含有乙醇作为主要碳源的培养基上培养使用乙醇并具有产生L-色氨酸的能力的沙雷氏菌属的微生物，并从所述培养基中回收累积的L-色氨酸 文化。

公开(公告)号：US3649457A

公开(公告)日：1972-03-14

申请号：US3649457D

申请日：1968-09-27

申请人： MONSANTO CO

发明人： WESTMAN THOMAS L

IPC分类号： B01J23/48 ,  C08F8/00 ,  C12N11/08 ,  C12P1/00 ,  C12P7/64 ,  C12P13/00 ,  C12P13/20 ,  C12P13/22 ,  C12P19/14 ,  C07G7/02 ,  C12K1/00

CPC分类号： C12P19/14 ,  B01J23/48 ,  C12N11/08 ,  C12P1/00 ,  C12P7/6418 ,  C12P13/004 ,  C12P13/20 ,  C12P13/227 ,  Y02P20/582 ,  Y10S435/813

摘要： POLYMER-ENXYME PRODUCTS, IN WHICH ENZYME IS COVALENTLY BOUND, ARE EMPLOYED IN PROCESSING BY CONTAINNG THEM, PREFERABLY IN SOLUTION, IN A REACTION ZONE, AT LEAST ONE BOUNDARY OF WHICH IS DEFINED BY A SEMI-PERMEABLE MEMBRANE HAVING EXCLUSION LIMITS SELECTED TO EXCLUDE PASSAGE THERETHROUGH OF ENZMY-POLYMER PRODUCT (EPP), ESPECIALLY WATER-SOLUBLE ENZYME-POLYMER PRODUCT (SEPP), BUT ALLOW PASSAGE THERETHROUGH OF SMALLER MOLECULAR WEIGHT REACTION PRODUCTS. EXCLUSION LMITS OF MEMBRANE MAY ALSO PREVENT PASSAGE THERETHROUGH OF SUBSTRATE, WHICH IS INTRODUCED INTO THE REACTION ZONE AND CONTACTED WITH EPP, ESPECIALLY SEPP, THEREIN. PRODUCTS OF THE ENZYMATIC REACTION PASS THROUGH THE MEMBRANE AND ARE RECOVERED. ADDITIONAL SUBSTRATE MAY THEN BE INTRODUCED INTO REACTION ZONE. EPP, INCLUDING SEPP, MAY BE RECYCLED IN THE PROCESS.

公开(公告)号：US2999051A

公开(公告)日：1961-09-05

申请号：US63845657

申请日：1957-02-06

申请人： LILLY CO ELI

发明人： BERNARD MALIN

IPC分类号： C12P13/22

CPC分类号： C12P13/227 ,  Y10S435/911 ,  Y10S435/939