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1.发明授权Method of treating microorganism cells containing tryptophanase or treated product thereof 失效处理含有色氨酸酶或其处理产物的微生物细胞的方法
公开(公告)号：US5256551A
公开(公告)日：1993-10-26
申请号：US731309
申请日：1991-07-17
申请人： Masato Terasawa , Mitsunobu Shimazu , Fuzio Endo , Hideaki Yukawa
发明人： Masato Terasawa , Mitsunobu Shimazu , Fuzio Endo , Hideaki Yukawa
CPC分类号： C12P13/227 , C12N1/00 , C12N9/99 , Y10S435/849
摘要： A method of selectively inhibiting pyruvic acid decomposition activity in microorganism cells containing tryptophanase or a treated product thereof, which comprises heat-treating said cells or the treated product thereof in the presence of an ammonium ion.
摘要翻译： 选择性抑制含有色氨酸酶或其处理产物的微生物细胞中的丙酮酸分解活性的方法，其包括在铵离子存在下热处理所述细胞或其处理产物。
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2.发明授权Method for producing L-threonine, and plasmid and microorganism employed in the same 失效生产L-苏氨酸的方法，以及在其中使用的血浆和微生物
公开(公告)号：US5153123A
公开(公告)日：1992-10-06
申请号：US257524
申请日：1988-10-14
申请人： Masato Terasawa , Terukazu Nara , Makiko Fukushima , Yukie Satoo , Mitsunobu Shimazu , Hideaki Yukawa , Yasurou Kurusu , Keiko Kohama
发明人： Masato Terasawa , Terukazu Nara , Makiko Fukushima , Yukie Satoo , Mitsunobu Shimazu , Hideaki Yukawa , Yasurou Kurusu , Keiko Kohama
CPC分类号： C12P13/08 , C12N15/52 , C12N15/77 , Y10S435/84
摘要： A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.
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3.发明授权L-alanine production with two microorganisms having fumarase inactivity in a single reaction tank 失效在单反应罐中具有两种具有不饱和脂肪酸的微生物的L-阿兰生产
公开(公告)号：US5116743A
公开(公告)日：1992-05-26
申请号：US790063
申请日：1991-11-12
申请人： Makoto Goto , Terukazu Nara , Masato Terasawa , Hideaki Yukawa
发明人： Makoto Goto , Terukazu Nara , Masato Terasawa , Hideaki Yukawa
IPC分类号： C12P13/06
CPC分类号： C12P13/06 , Y10S435/874 , Y10S435/876 , Y10S435/877
摘要： There is disclosed a process for producing L-alanine by reacting in a single reaction tank, in an aqueous reaction mixture having a pH of 6 to 10 and containing at least one .alpha.-keto acid, fumaric acid or a salt thereof with ammonia or ammonium ions in the presence of two microorganisms having fumarase inactivity.
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公开(公告)号：US5738993A
公开(公告)日：1998-04-14
申请号：US662963
申请日：1996-06-13
CPC分类号： C12Q1/6876 , C12Q1/6827
摘要： An oligonucleotide comprising a specific region and one or two non-specific regions which are bound to at least one of the two termini of the specific region, wherein the specific region has a specific base sequence which is substantially complementary to a target sequence of a sample nucleic acid with which the oligonucleotide is to be hybridized, the non-specific region is composed of at least one nucleotide having other base capable of forming a base pair with each of bases constituting a common nucleic acid, or its oligomer, is used as a probe for the hybridization.
摘要翻译： 包含与特定区域的两个末端中的至少一个结合的特定区域和一个或两个非特异性区域的寡核苷酸，其中特异性区域具有与样品的靶序列基本上互补的特异性碱基序列 寡核苷酸要与其杂交的核酸，非特异性区域由至少一个能够与构成共同核酸的每个碱基形成碱基对的其他碱基的核苷酸或其低聚物用作 探针进行杂交。
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公开(公告)号：US5776740A
公开(公告)日：1998-07-07
申请号：US682193
申请日：1996-07-17
CPC分类号： C12P13/22
摘要： The present invention relates to a process for producing L-tryptophan in a single-stage reaction, comprising carrying out an L-tryptophan producing reaction with glycine, formaldehyde and indole as raw materials in an aqueous solution in the presence of microbial cells having serine transhydroxymethylase or a treated products thereof and microbial cells having tryptophan synthase or tryptophanase, or a treated products thereof; and collecting produced L-tryptophan from the reaction solution.
摘要翻译： 本发明涉及在单阶段反应中生产L-色氨酸的方法，包括在具有丝氨酸转羟甲基化酶的微生物细胞存在下，在水溶液中以甘氨酸，甲醛和吲哚为原料进行产生L-色氨酸的反应 或其处理产物和具有色氨酸合酶或色氨酸酶的微生物细胞或其处理产物; 并从反应溶液中收集产生的L-色氨酸。
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6.发明授权Process for culturing microorganisms of the genus Pseudomonas and process for producing L-Alanine using said microorganisms 失效培养基因组培养微生物的方法和使用微生物生产L-赖氨酸的方法
公开(公告)号：US5149651A
公开(公告)日：1992-09-22
申请号：US691880
申请日：1991-04-26
申请人： Makoto Goto , Terukazu Nara , Yasukazu Uchida , Masato Terasawa , Hideaki Yukawa , Hisashi Yamagata
发明人： Makoto Goto , Terukazu Nara , Yasukazu Uchida , Masato Terasawa , Hideaki Yukawa , Hisashi Yamagata
CPC分类号： C12P13/06 , Y10S435/874 , Y10S435/876 , Y10S435/877
摘要： Microorganisms of the genus Pseudomonas containing aspartate beta-decarboxylase at a high level are produced by culturing said microorganism in a medium supplemented with a chelating agent. L-Alanine can be efficiently produced from L-aspartic acid said microorganisms or the treated product thereof.
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公开(公告)号：US5783428A
公开(公告)日：1998-07-21
申请号：US804144
申请日：1997-02-20
申请人： Makoto Goto , Terukazu Nara , Izuru Tokumaru , Nobutake Fugono , Yasukazu Uchida , Masato Terasawa , Hideaki Yukawa
发明人： Makoto Goto , Terukazu Nara , Izuru Tokumaru , Nobutake Fugono , Yasukazu Uchida , Masato Terasawa , Hideaki Yukawa
CPC分类号： C12P7/46 , C12P13/20 , Y10S435/832 , Y10S435/833
摘要： Fumaric acid is produced by reacting a culture of a microorganism which produces maleate isomerase that exhibits a maximum activity at not less than 50.degree. C. or a treated product thereof with maleic acid in an aqueous solution, and isomerizing maleic acid to produce fumaric acid. L-aspartic acid is produced by reacting both of a culture of a microorganism which produces maleate isomerase or a treated product thereof and a culture of a microorganism which produces aspartase or a treated product thereof with maleic acid and ammonia in an aqueous solution, producing L-aspartic acid from maleic acid and ammonia by enzyme reactions, and recovering L-aspartic acid from the reaction mixture.
摘要翻译： 富马酸是通过使生成马来酸异构酶的微生物培养物（其在50℃以上表现出最大活性）或其处理产物与马来酸在水溶液中反应，并使马来酸异构化以产生富马酸而制备的。 L-天冬氨酸通过使水解产生马来酸异构酶的微生物培养物或其处理产物和产生天冬氨酰胺的微生物培养物或其处理产物与马来酸和氨反应，生成L - 天冬氨酸通过酶反应从马来酸和氨中回收，并从反应混合物中回收L-天冬氨酸。
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公开(公告)号：US5677156A
公开(公告)日：1997-10-14
申请号：US623001
申请日：1996-03-28
申请人： Makoto Goto , Izuru Tokumaru , Masato Terasawa , Hideaki Yukawa
发明人： Makoto Goto , Izuru Tokumaru , Masato Terasawa , Hideaki Yukawa
CPC分类号： C12P7/46 , C12P13/20 , Y10S435/829 , Y10S435/84
摘要： Fumaric acid is produced by reacting maleic acid in an aqueous solution with a microorganism which has maleate isomerase activity or with a preparation from the microorganism having the maleate isomerase activity, and producing fumaric acid in a reaction solution by enzymatic isomerization of maleic acid carried out under the condition that the dissolved oxygen concentration in the reaction solution is substantially maintained at 4 ppm or less, for example, by sealing the reaction solution with one or more gases selected from N.sub.2, Ar, and He.
摘要翻译： 通过马来酸在水溶液中与具有马来酸异构酶活性的微生物或具有马来酸异构酶活性的微生物的制剂反应制备富马酸，并通过在下面进行的马来酸的酶促异构化在反应溶液中产生富马酸 反应溶液中的溶解氧浓度基本上保持在4ppm以下的条件，例如通过用一种或多种选自N 2，Ar和He的气体密封反应溶液。
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公开(公告)号：US5496715A
公开(公告)日：1996-03-05
申请号：US293969
申请日：1994-08-24
申请人： Makoto Goto , Terukazu Nara , Masato Terasawa , Hideaki Yukawa
发明人： Makoto Goto , Terukazu Nara , Masato Terasawa , Hideaki Yukawa
CPC分类号： C12R1/01 , C12P17/165 , Y10S435/822
摘要： There is disclosed a process for preparing indigo which comprises bringing an indigo-producing bacteria belonging to genus Acinetobacter or treated products thereof into contact with an aqueous solution containing at least indole to have indigo formed and accumulated in the aqueous solution; and collecting indigo from the aqueous solution.
摘要翻译： 公开了制备靛蓝的方法，其包括将属于不动杆菌属的靛蓝生产细菌或其处理产物与至少含有吲哚的水溶液接触以形成并积聚在水溶液中的靛蓝; 并从水溶液中收集靛蓝。
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公开(公告)号：US5279951A
公开(公告)日：1994-01-18
申请号：US519776
申请日：1990-05-07
IPC分类号： C12P13/22 , C12N1/21 , C12N9/88 , C12N15/09 , C12N15/60 , C12N15/70 , C12R1/19 , C12N15/11 , C12N15/71
CPC分类号： C12N9/88 , C12P13/227 , Y10S435/849
摘要： A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.
摘要翻译： 培养用重组质粒转化的微生物的方法，所述重组质粒至少含有（a）含有启动子的DNA片段和位于启动子下游的色氨酸酶操纵子（tna）中的调节基因tnaC，和（b）含有 可以由启动子表达的所需结构基因，其包括在连续或间歇地添加作为碳源的葡萄糖的同时在培养基中培养转化的微生物，使得葡萄糖的浓度保持在0.01〜0.3％的范围内，由此 使所需的结构基因在微生物中表达。
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