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58 条记录 (耗时 0.023 秒)

    Method of testing for periodontal disease
    41.
    发明授权
    Method of testing for periodontal disease 失效
    牙周病检测方法

    公开(公告)号：US06277587B1

    公开(公告)日：2001-08-21

    申请号：US09308215

    申请日：1999-05-14

    申请人： Ira B. Lamster

    发明人： Ira B. Lamster

    IPC分类号： C12Q134

    CPC分类号： C12Q1/34 ,  C12Q2334/22 ,  G01N2333/924

    摘要： This invention provides a method of testing for periodontal disease in a subject which comprises detecting an elevated concentration of &bgr;-glucuronidase in saliva from the subject relative to the concentration of &bgr;-glucuronidase present in saliva from a healthy subject. The concentration of &bgr;-glucuronidase in the subject's saliva is determined by adding to a sample of the saliva a substrate for &bgr;-glucuronidase and measuring the amount of a product produced by the action of &bgr;-glucuronidase in the substrate. Also, the concentration of &bgr;-glucuronidase in the subject's saliva is determined by adding to a sample of saliva a labeled antibody specific for &bgr;-glucuronidase and measuring the amount of labeled antibody which forms a complex with &bgr;-glucuronidase present in the saliva.

    摘要翻译： 本发明提供了一种测试受试者中牙周病的方法，其包括检测来自受试者的唾液中β-葡糖醛酸糖苷酶的浓度相对于存在于来自健康受试者的唾液中的β-葡糖醛酸糖苷酶的浓度。 通过向唾液中加入β-葡糖醛酸糖苷酶底物并测量通过β-葡糖醛酸糖苷酶在底物中的作用产生的产物的量来测定受试者唾液中β-葡糖醛酸糖苷酶的浓度。 此外，通过向唾液样品添加对β-葡糖醛酸糖苷酶特异性的标记抗体并测量与存在于唾液中的β-葡糖醛酸糖苷酶形成复合物的标记抗体的量来确定受试者唾液中β-葡糖醛酸糖苷酶的浓度。

    Methods and pharmaceutical compositions for inhibiting tumor cell growth
    43.
    发明授权
    Methods and pharmaceutical compositions for inhibiting tumor cell growth 有权
    用于抑制肿瘤细胞生长的方法和药物组合物

    公开(公告)号：US06242196B1

    公开(公告)日：2001-06-05

    申请号：US09319769

    申请日：1999-09-17

    申请人： Bruce M. Spiegelman ,  Soner Altiok ,  Elisabetta Mueller ,  Pasha Sarraf ,  Peter Tontonoz

    发明人： Bruce M. Spiegelman ,  Soner Altiok ,  Elisabetta Mueller ,  Pasha Sarraf ,  Peter Tontonoz

    IPC分类号： C12Q134

    CPC分类号： A61K31/426

    摘要： A method for inhibiting proliferation of a PPAR &ggr;-responsive hyperproliferative cell which comprises the step of contacting the cell with (I) an inhibitory amount of a PPAR&ggr; agonist and (II) a MAP kinase inhibitor is disclosed. A method for treating or prophylactically preventing in an animal subject a disorder characterized by unwanted proliferation of PPAR&ggr;-responsive hyperproliferative cells which comprises administering to the subject (I) an inhibitory amount of a PPAR&ggr; agonist and (II) a MAP kinase inhibitor is also disclosed. Pharmaceutical compositions comprising a therapeutically effective amount of a PPAR&ggr; agonist and a MAP kinase inhibitor are disclosed for use in the methods.

    摘要翻译： 公开了一种抑制PPARγ反应性过度增殖细胞增殖的方法，其包括使细胞与（I）抑制量的PPARγ激动剂和（II）MAP激酶抑制剂接触的步骤。 一种用于在动物受试者中治疗或预防性地预防PPARγ反应性过度增殖性细胞不希望的增殖的病症的方法，其包括对受试者（I）施用抑制量的PPARγ激动剂和（II）MAP激酶抑制剂 。 公开了包含治疗有效量的PPARγ激动剂和MAP激酶抑制剂的药物组合物用于该方法。

    Positive response biosensors and other sensors
    45.
    发明授权
    Positive response biosensors and other sensors 有权
    正响应生物传感器等传感器

    公开(公告)号：US06750033B2

    公开(公告)日：2004-06-15

    申请号：US09850686

    申请日：2001-05-07

    申请人： Keith E. LeJeune ,  Markus Erbeldinger

    发明人： Keith E. LeJeune ,  Markus Erbeldinger

    IPC分类号： C12Q134

    CPC分类号： C12Q1/001 ,  G01N33/5438 ,  G01N33/94 ,  Y10S435/963

    摘要： A sensor for detecting an analyte in an environment includes a first reaction system including a first enzyme and a substrate for the first enzyme. The analyte inhibits the reaction of the substrate catalyzed by the first enzyme (in other words, the analyte inhibits the first enzyme). The sensor further includes at least a second reaction system that reacts to produce a first detectable state when the first enzyme is inhibited. In some embodiments, the reaction of the first reaction system can produce a second detectible state, different from the first detectible state. Another sensor for detecting an analyte in an environment includes a first reaction system including a first enzyme or a first substrate for the first enzyme. In this embodiment, the analyte is either a substrate for the first enzyme if the first reaction system includes the first enzyme or the first enzyme if the first reaction system includes the first substrate. The sensor also includes at least a second reaction system that reacts to produce a first detectable state when the analyte is below a certain concentration. The sensor thus provides a positive or detectible response when the analyte is absent or deficient. Once again, the first reaction can produce a second detectible state, different from the first detectible state.

    摘要翻译： 用于检测环境中的分析物的传感器包括包含第一酶和第一酶底物的第一反应体系。 分析物抑制由第一酶催化的底物的反应（换句话说，分析物抑制第一种酶）。 所述传感器还包括至少第二反应体系，当第一酶被抑制时，所述第二反应系统反应以产生第一可检测状态。 在一些实施方案中，第一反应体系的反应可产生与第一可检测状态不同的第二可检测状态。 用于检测环境中的分析物的另一传感器包括包含第一酶或第一酶的第一底物的第一反应系统。 在该实施方案中，如果第一反应体系包括第一种酶或第一种酶，如果第一种反应体系包括第一种底物，则分析物是第一种酶的底物。 该传感器还包括至少一个第二反应系统，当分析物低于一定浓度时，其反应产生第一可检测状态。 因此，当分析物不存在或不足时，传感器提供了正面或可检测的响应。 再次，第一反应可以产生与第一可检测状态不同的第二可检测状态。

    Method of measuring total homocysteine
    46.
    发明授权
    Method of measuring total homocysteine 失效
    测量总同型半胱氨酸的方法

    公开(公告)号：US06686172B2

    公开(公告)日：2004-02-03

    申请号：US10069847

    申请日：2002-02-28

    申请人： Naoto Matsuyama ,  Mina Fukuhara ,  Masaharu Takayama ,  Koji Mizuno

    发明人： Naoto Matsuyama ,  Mina Fukuhara ,  Masaharu Takayama ,  Koji Mizuno

    IPC分类号： C12Q134

    CPC分类号： G01N33/6815 ,  C12Q1/26 ,  C12Q1/34 ,  Y10S435/968 ,  Y10S435/975

    摘要： The present invention provides a method and a kit for detecting or quantitatively determining homocysteine rapidly and simply and with high sensitivity by oxidizing the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof in the presence of an SH reagent to produce hydrogen peroxide and determining the produced hydrogen peroxide by color development using an oxidative color-developing agent. By using the method and kit of the present invention, homocysteine in biological samples, in particular, in body fluids such as blood and urine can be detected and quantitatively determined rapidly and simply and with high sensitivity.

    摘要翻译： 本发明提供一种方法和试剂盒，用于通过在SH试剂存在下氧化残留的同型半胱氨酸共底物，产生的同型半胱氨酸转化酶产物或其酶反应产物，快速且简单地并且具有高灵敏度来检测或定量测定同型半胱氨酸， 产生过氧化氢，并使用氧化显色剂通过显色测定产生的过氧化氢。 通过使用本发明的方法和试剂盒，生物样品中的同型半胱氨酸，特别是体液如血液和尿液中的高半胱氨酸可以快速且简单且高灵敏度地进行检测和定量测定。

    Motor proteins and methods for their use
    48.
    发明授权
    Motor proteins and methods for their use 失效

    公开(公告)号：US06548267B1

    公开(公告)日：2003-04-15

    申请号：US09723595

    申请日：2000-11-28

    申请人： Christophe Beraud

    发明人： Christophe Beraud

    IPC分类号： C12Q134

    CPC分类号： C12N9/14 ,  Y10S977/914

    摘要： The present invention provides high throughput screening systems for identifying compounds useful in the treatment of cellular proliferation disorders. The method can be performed in plurality simultaneously with fluorescence or absorbance readouts.

    Hydrolytic enzyme substrates and assay method
    49.
    发明授权
    Hydrolytic enzyme substrates and assay method 失效
    水解酶底物和测定方法

    公开(公告)号：US06455268B1

    公开(公告)日：2002-09-24

    申请号：US09706342

    申请日：2000-11-03

    申请人： Kurt Jarnigan ,  Roger Leger ,  Ving J. Lee ,  David Morgans

    发明人： Kurt Jarnigan ,  Roger Leger ,  Ving J. Lee ,  David Morgans

    IPC分类号： C12Q134

    CPC分类号： C12Q1/34 ,  C12Q1/00 ,  G01N33/582 ,  Y10S435/975

    摘要： Compounds of the invention have the structure D—L—A*, where L is a linking moiety that maintains D and A in a configuration compatible with FRET, D is a fluorescent donor moiety capable of causing an acceptor moiety to fluoresce by FRET, and A* is a fluorescent acceptor moiety capable of accepting energy from the donor moiety by FRET and fluorescing only after cleavage of one or more labile groups to provide acceptor A. These compounds are useful as fluorescent substrates for detection of enzymes in vivo.

    摘要翻译： 本发明的化合物具有结构DLA *，其中L是维持与FRET相容的构型的D和A的连接部分，D是能够通过FRET使受体部分发荧光的荧光供体部分，A *是 能够通过FRET接受来自供体部分的能量的荧光受体部分，并且仅在裂解一个或多个不稳定基团以提供受体A之后发荧光。这些化合物可用作体内检测酶的荧光底物。