公开(公告)号：US09957481B2

公开(公告)日：2018-05-01

申请号：US13944776

申请日：2013-07-17

Applicant: Academia Sinica

Inventor： Keng-Hui Lin ,  Narayan Mishra ,  Yen-Liang Liu ,  Chen-Chie Wang

IPC: C12N5/077 ,  B29C44/00 ,  C12N5/00 ,  C12M1/12

CPC classification number: C12N5/0655 ,  B29C44/00 ,  C12M25/14 ,  C12N5/0068 ,  C12N2533/74

Abstract: Methods and apparatuses for using microfluidics to generate bubbles and using the generated bubbles to construct scaffolds and cell-holding structures for culturing biological samples or analytes. In one implementation, a scaffold for growing cells is provided to include a matrix of interconnected cavities formed from mixing a gas and a liquid containing a cross linkable material to produce a matrix of gas bubbles of substantially the same size and cross linking the cross linkable material to form a structure in which cells are grown. In another implementation, a scaffold apparatus for growing cells includes a ball of a cross linked material forming an exterior shell that encloses to form a hollow interior inside the ball and biological samples embedded in the external shell.

公开(公告)号：US20180092846A1

公开(公告)日：2018-04-05

申请号：US15567837

申请日：2016-04-20

Applicant: Academia Sinica

Inventor： Patrick C.H Hsieh ,  Bill Cheng

IPC: A61K9/127 ,  A61K31/555

CPC classification number: A61K9/1271 ,  A61K9/0019 ,  A61K9/1277 ,  A61K9/5068 ,  A61K31/409 ,  A61K31/555

Abstract: Proteo-microparticles such as proteoliposomes comprising a microparticle (e.g., a liposome) and platelet membrane proteins, wherein the proteo-microparticles are capable of binding to monocytes, neutrophils, or other circulating blood cells capable of migrating to an injured site. Also provided herein are uses of the proteoliposomes for delivering a therapeutic agent via monocytes to an injured site.

公开(公告)号：US09925235B2

公开(公告)日：2018-03-27

申请号：US15137281

申请日：2016-04-25

Applicant: Academia Sinica ,  National Taiwan Ocean University

Inventor： Jyh-Yih Chen ,  Chang-Jer Wu

IPC: A61K38/16 ,  A61K38/17 ,  C07K14/00 ,  C07K4/12

CPC classification number: A61K38/1706

Abstract: A method for enhancing wound healing or treating methicillin-resistant Staphylococcus aureus (MRSA) infection in a wound comprising administering a subject in need thereof with a therapeutically effective amount of a tilapia piscidin (TP) is described, wherein the TP is preferably TP3 or TP4.

公开(公告)号：US09915614B2

公开(公告)日：2018-03-13

申请号：US13872013

申请日：2013-04-26

Applicant: Academia Sinica ,  Max-Planck-Institut Für Eisenforschung GmbH

Inventor： Chia-Fu Chou ,  Lesser-Rojas Leonardo ,  Ming-Lee Chu ,  Andreas Erbe

IPC: G01N21/00 ,  G01N21/65 ,  B03C5/02 ,  G01N33/487

CPC classification number: G01N21/65 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/48721

Abstract: Methods, systems, and devices are disclosed for molecular capture, manipulation, and analysis. In one aspect, a device to aggregate and characterize particles in a fluid includes an electrically insulative substrate including a channel to carry an electrically conducting fluid containing particles, electrodes located in the channel forming a nanoscale opening and including an insulating layer over their surface at the opening, a first circuit to apply a non-uniform ac electric field and a dc bias signal across the electrodes, in which the applied non-uniform ac electric field produces a positive dielectrophoretic force to aggregate the particles in a trapping region including the opening and adjacent region, a second circuit to detect changes in a dc current caused by at least some of the particles in the trapping region, and an optical device that directs a coherent light beam on the opening to determine Raman spectra of the particles in the trapping region.

公开(公告)号：US20180044437A1

公开(公告)日：2018-02-15

申请号：US15721970

申请日：2017-10-02

Applicant: Academia Sinica

Inventor： Tse-Wen CHANG ,  Jou-Han CHEN ,  Hsing-Mao CHU

IPC: C07K16/46

CPC classification number: A61K47/6803 ,  A61K31/397 ,  A61K31/4545 ,  A61K31/4709 ,  A61K31/4745 ,  A61K31/537 ,  A61K31/739 ,  A61K47/58 ,  A61K47/60 ,  A61K47/61 ,  A61K47/64 ,  A61K47/6801 ,  A61K47/6843 ,  A61K47/6845 ,  A61K47/6849 ,  A61K47/6851 ,  A61K47/6883 ,  A61K51/065 ,  A61K51/088 ,  A61K2039/505 ,  C07K14/485 ,  C07K14/655 ,  C07K14/705 ,  C07K14/70578 ,  C07K14/7151 ,  C07K16/18 ,  C07K16/22 ,  C07K16/241 ,  C07K16/244 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2818 ,  C07K16/2863 ,  C07K16/2875 ,  C07K16/2887 ,  C07K16/32 ,  C07K16/468 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/64 ,  C07K2317/71 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/76 ,  C07K2317/94 ,  C07K2319/30 ,  C07K2319/32 ,  C07K2319/33

Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating osteoporosis using such molecular constructs are also disclosed.

公开(公告)号：US20180030473A1

公开(公告)日：2018-02-01

申请号：US15723312

申请日：2017-10-03

Applicant: Academia Sinica

Inventor： SWEE-SUAK KO ,  MIN-JENG LI

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8289 ,  C07K14/415

Abstract: The present invention relates to a method for producing male sterile plant, a mutated nucleotide molecule comprising a nucleotide sequence of the transcription factor bHLH142 and an inserted T-DNA segment, and a novel transformed plant cell and a male-sterile mutant plant comprising the mutated nucleotide molecule, in which the transcription factor bHLH142 is not expressed. The present invention also relates to a novel reversible male sterile transgenic plant, wherein the transcription factor bHLH142 is overexpressed, and its preparation method. The bHLH gene is tissue specifically expresses in the anther and it plays a pivotal role in pollen development. Both the male sterile and reversible male sterile transgenic plants showed a completely male sterile phenotype, but the fertility of the reversible male sterile transgenic plant can be restored under low temperature.

公开(公告)号：US09879042B2

公开(公告)日：2018-01-30

申请号：US14847835

申请日：2015-09-08

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: C07H17/02 ,  C07H15/18 ,  A61K39/39 ,  C07H15/04 ,  A61K39/00

CPC classification number: C07H15/18 ,  A61K31/7032 ,  A61K39/39 ,  A61K2039/55511 ,  A61K2039/585 ,  C07H15/04 ,  C07H17/02

Abstract: Glycosphingolipids (GSLs) compositions and methods for iNKT-independent induction of chemokines are disclosed.

公开(公告)号：US20180025108A1

公开(公告)日：2018-01-25

申请号：US15670478

申请日：2017-08-07

Applicant: ACADEMIA SINICA

Inventor： An-Suei YANG ,  Hung-Pin PENG ,  Jhih-Wei JIAN

IPC: G06F19/12 ,  G06F19/24

CPC classification number: G16B5/00 ,  G16B40/00 ,  G16B50/00

Abstract: In a general aspect, a method for inferring one or more biomolecule-to-biomolecule interaction sites includes receiving data representative of a plurality of prediction models. Each prediction model is associated with a different atom type of a plurality of atom types and characterizes biomolecule-to-biomolecule interaction site specific patterns common to a plurality of three dimensional probability density maps. Each three dimensional probability density map is associated with a corresponding biomolecule of a plurality of biomolecules included in a training data set and represents a probability of a non-covalent interacting atom on a surface of the corresponding biomolecule interacting with the atom type associated with the prediction model. Data representative of a query biomolecule is received, the data including one or more unknown biomolecule-to-biomolecule interaction sites. The one or more unknown biomolecule-to-biomolecule interaction sites of the query biomolecule are inferred based on the data representative of the plurality of prediction models.

公开(公告)号：US09856480B2

公开(公告)日：2018-01-02

申请号：US14760904

申请日：2014-01-14

Applicant: DCB-USA LLC ,  NATIONAL TAIWAN UNIVERSITY ,  ACADEMIA SINICA

Inventor： Pan-Chyr Yang ,  Wei-Yun Lai ,  Konan Peck ,  Cheng-Ju Chang ,  Chi-Yuan Chen ,  Shuenn-Chen Yang

IPC: A61K48/00 ,  C12N15/11 ,  C07H21/02 ,  C07H21/04 ,  C12N15/113 ,  C07K14/485 ,  A61K31/517 ,  A61K31/713

CPC classification number: C12N15/1138 ,  A61K31/517 ,  A61K31/713 ,  C07K14/485 ,  C12N2310/127 ,  C12N2310/315 ,  C12N2310/33 ,  C12N2310/3515 ,  C12N2320/31 ,  C12N2320/34

Abstract: The invention provides DNAzymes which are capable to silence the expression of EGFR at allele-specific level. These allele-specific DNAzymes against EGFR T790M mutation will knockdown the expression of EGFR T790M mRNA while keeping EGFR wild-type mRNA intact. Hence, these allele-specific DNAzymes against EGFR T790M mutation may overcome T790M-derived TKI resistance accompanied with lower unwanted side effects on normal cells in lung cancer patients.

公开(公告)号：US20170360884A1

公开(公告)日：2017-12-21

申请号：US15537051

申请日：2015-12-29

Applicant: ACADEMIA SINICA

Inventor： Shie-Liang HSIEH ,  Yung-Chi CHANG ,  Ming-Ting HUANG

IPC: A61K38/17 ,  C07K14/705

CPC classification number: A61K38/177 ,  C07K14/70578 ,  C07K2319/30

Abstract: Disclosed herein is a method of treating influenza A virus (IAV) infection by a fusion protein. According to some embodiments of the present disclosure, the fusion protein comprises a HBD peptide and a IgG1 Fc region. According to other embodiments of the present disclosure, the fusion protein comprises a DcR3 protein and a IgG1 Fc region. The present fusion protein is found to possess inhibitory effects on IAV-induced secretion of the inflammatory cytokine, and IAV-induced infiltration of inflammatory cell into the lung tissue. Accordingly, the fusion protein is useful for developing a medicament for the treatment or prophylaxis of IAV infection and/or ameliorating pulmonary injury caused by excessive inflammation associated with IAV infection in a subject.