公开(公告)号：US20180371536A1

公开(公告)日：2018-12-27

申请号：US15574122

申请日：2016-05-26

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Julie Wilhelmy

IPC: C12Q1/6851 ,  C07H21/02

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for quantification of RNA, and determination of efficiency of re verse transcription of RNA to cDNA.

公开(公告)号：US10151003B2

公开(公告)日：2018-12-11

申请号：US16038979

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180346970A1

公开(公告)日：2018-12-06

申请号：US15937713

申请日：2018-03-27

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6804

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US10047394B2

公开(公告)日：2018-08-14

申请号：US15224460

申请日：2016-07-29

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Glenn K. Fu

IPC: C12N15/10 ,  G06F19/20 ,  G06F19/22 ,  C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q1/6851

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B30/00 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US10002316B2

公开(公告)日：2018-06-19

申请号：US15667125

申请日：2017-08-02

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06 ,  C12Q1/68

CPC classification number: G06K19/06103 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q2525/179 ,  C12Q2543/10 ,  C12Q2563/155

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

公开(公告)号：US20170344866A1

公开(公告)日：2017-11-30

申请号：US15605874

申请日：2017-05-25

Applicant: Cellular Research, Inc.

Inventor： Jue Fan ,  Jennifer Tsai ,  Eleen Shum ,  Lisha Deng ,  Glenn K. Fu

IPC: G06K19/06

CPC classification number: G06K19/06028 ,  G16B30/00 ,  G16B40/00

Abstract: Disclosed herein are methods and systems for determining the numbers of targets. In some embodiments, the method comprise: stochastically barcoding targets using stochastic barcodes; obtaining sequencing data; for one or more of the targets: counting the number of molecular labels with distinct sequences associated with the target in the sequencing data; identifying clusters of molecular labels of the target using directional adjacency; collapsing the sequencing data using the clusters of molecular labels of the target identified; and estimating the number of the target.

公开(公告)号：US20170342405A1

公开(公告)日：2017-11-30

申请号：US15596364

申请日：2017-05-16

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Eleen Shum

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.

公开(公告)号：US09567645B2

公开(公告)日：2017-02-14

申请号：US14472363

申请日：2014-08-28

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

Abstract translation: 本公开提供了用于单个细胞的多重核酸分析的方法，组合物和试剂盒。 方法，组合物和系统可用于大规模并行单细胞测序。 方法，组成和系统可用于同时分析数千个细胞。 数千个细胞可以包含混合的细胞群体（例如，不同类型或亚型的细胞，不同大小的细胞）。

公开(公告)号：US20160376648A1

公开(公告)日：2016-12-29

申请号：US15217886

申请日：2016-07-22

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B30/00 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US20160326584A1

公开(公告)日：2016-11-10

申请号：US15224460

申请日：2016-07-29

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.