公开(公告)号：US20180088112A1

公开(公告)日：2018-03-29

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  C12Q1/68 ,  G01N33/543 ,  C12N15/10

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US11661631B2

公开(公告)日：2023-05-30

申请号：US16747737

申请日：2020-01-21

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6853

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2531/113 ,  C12Q2563/185

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US10676779B2

公开(公告)日：2020-06-09

申请号：US15937713

申请日：2018-03-27

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6804 ,  C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6876 ,  G01N33/532 ,  C12Q1/6855 ,  C12Q1/6865 ,  B82Y5/00 ,  C12Q1/6837 ,  C12Q1/6834

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

公开(公告)号：US20200232032A1

公开(公告)日：2020-07-23

申请号：US16747737

申请日：2020-01-21

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Katherine Lazaruk ,  Dennis E. Prosen ,  Devon Jensen ,  David Rosenfeld ,  Janice H. Lai ,  James Ghadiali ,  Christina Chang

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6853

Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).

公开(公告)号：US11460468B2

公开(公告)日：2022-10-04

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B25/10 ,  G16H20/13 ,  G16H10/20

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20180346970A1

公开(公告)日：2018-12-06

申请号：US15937713

申请日：2018-03-27

Applicant: CELLULAR RESEARCH, INC.

Inventor： Christina Chang ,  Christina Fan ,  Eleen Shum ,  Jody Martin ,  Nidhanjali Bansal ,  James Ghadiali ,  Katherine Lazaruk ,  Gretchen Yinbon Lam

IPC: C12Q1/6816 ,  C12Q1/6806 ,  C12Q1/6811 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6804

Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.