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公开(公告)号：US09453256B2
公开(公告)日：2016-09-27
申请号：US15009304
申请日：2016-01-28
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US09447459B2
公开(公告)日：2016-09-20
申请号：US14166218
申请日：2014-01-28
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US20160258006A1
公开(公告)日：2016-09-08
申请号：US15165921
申请日：2016-05-26
Applicant: Keygene N.V.
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G06F19/22 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
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公开(公告)号：US20160255833A1
公开(公告)日：2016-09-08
申请号：US15155812
申请日：2016-05-16
Applicant: Keygene N.V.
Inventor： Petronella Martina BLEEKER , Kai AMENT , Paul Johan DIERGAARDE , Michiel Theodoor Jan DE BOTH , Robert Cornelis SCHUURINK
IPC: A01N27/00
CPC classification number: A01N27/00 , A01N49/00 , A01N25/02 , A01N2300/00
Abstract: The present invention provides compounds and compositions for attracting or repelling sap-sucking insects, such as whitefly, as well as methods for using such compositions.
Abstract translation: 本发明提供了用于吸引或排斥诸如粉虱之类的吸汁昆虫的化合物和组合物，以及使用这些组合物的方法。
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公开(公告)号：US20160251713A1
公开(公告)日：2016-09-01
申请号：US15014642
申请日：2016-02-03
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Taco Peter JESSE
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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Patent Agency Ranking
公开(公告)号：US09376716B2
公开(公告)日：2016-06-28
申请号：US14885063
申请日：2015-10-16
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US09328383B2
公开(公告)日：2016-05-03
申请号：US14699891
申请日：2015-04-29
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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公开(公告)号：US20160060686A1
公开(公告)日：2016-03-03
申请号：US14885063
申请日：2015-10-16
Applicant: KEYGENE N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US09090888B2
公开(公告)日：2015-07-28
申请号：US14571095
申请日：2014-12-15
Applicant: Keygene N.V.
CPC classification number: C12N15/8286 , A01H1/04 , C12N9/88 , C12N15/8239 , C12N15/8243 , C12P5/007 , C12Y402/00 , C12Y402/03053 , C12Y402/03054 , C12Y402/03082 , Y02A40/162
Abstract: The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
Abstract translation: 本公开提供了分离的编码7-表位活性成分合成酶的核酸分子，包含所述核酸分子的嵌合基因，包含该核酸分子的载体以及分离的7-表质酰基合成酶本身。 此外，提供了转基因植物和植物细胞，其包含编码7-表位活性物质合成酶的基因，任选地整合在其基因组中，以及用于制备这种植物和细胞的方法。 提供具有增强的害虫抗性的茄科植物和植物部分（种子，水果，叶子等）。
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60.发明授权公开(公告)号：US08975028B2
公开(公告)日：2015-03-10
申请号：US14219931
申请日：2014-03-19
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Taco Peter Jesse
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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