公开(公告)号：US5284761A

公开(公告)日：1994-02-08

申请号：US827728

申请日：1992-02-26

Applicant: Patrick Aebischer ,  Lars Wahlberg

Inventor： Patrick Aebischer ,  Lars Wahlberg

IPC: A61F2/02 ,  A61K9/00 ,  A61K9/48 ,  A61K35/12 ,  A61M31/00 ,  B01J13/02 ,  B01J13/04 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0793 ,  C12N11/04 ,  C12N11/08

CPC classification number: A61K9/0092 ,  A61F2/022 ,  A61K35/12 ,  A61K9/0024 ,  A61K9/0085 ,  A61K9/4816 ,  A61K9/4833 ,  A61M31/002 ,  B01J13/02 ,  B01J13/04 ,  C12N11/04 ,  C12N11/08 ,  C12N5/0012 ,  C12N5/0614 ,  C12N5/0619 ,  A61K2035/126 ,  A61K2035/128 ,  A61M2210/0687 ,  A61M2210/0693 ,  C12N2533/30

Abstract: Methods and systems are disclosed for encapsulating viable cells which produce biologically-active factors. The cells are encapsulated within a semipermeable, polymeric membrane by co-extruding an aqueous cell suspension and a polymeric solution through a common port to form a tubular extrudate having a polymeric outer coating which encapsulates the cell suspension. For example, the cell suspension and the polymeric solution can be extruded through a common extrusion port having at least two concentric bores, such that the cell suspension is extruded through the inner bore and the polymeric solution is extruded through the outer bore. The polymeric solution coagulates to form an outer coating. As the outer coating is formed, the ends of the tubular extrudate can be sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links.

Abstract translation: 公开了用于包封产生生物活性因子的活细胞的方法和系统。 通过将水性细胞悬浮液和聚合物溶液共挤出通过共同的口以形成具有封装细胞悬浮液的聚合物外涂层的管状挤出物，将细胞包封在半透膜聚合物膜内。 例如，细胞悬浮液和聚合物溶液可以通过具有至少两个同心孔的共同挤出口挤出，使得细胞悬浮液通过内孔挤出并且聚合物溶液通过外孔挤出。 聚合物溶液凝结形成外涂层。 当形成外涂层时，管状挤出物的端部可被密封以形成电池盒。 在一个实施方案中，管状挤出物间隔密封以限定通过聚合物连接物连接的分离的细胞室。

公开(公告)号：US5283187A

公开(公告)日：1994-02-01

申请号：US638759

申请日：1991-01-08

Applicant: Patrick Aebischer ,  Lars Wahlberg

Inventor： Patrick Aebischer ,  Lars Wahlberg

IPC: A61F2/02 ,  A61K9/00 ,  A61K9/48 ,  A61K35/12 ,  A61M31/00 ,  B01J13/02 ,  B01J13/04 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0793 ,  C12N11/04 ,  C12N11/08 ,  C12N11/12

CPC classification number: A61K9/0092 ,  A61F2/022 ,  A61K35/12 ,  A61K9/0024 ,  A61K9/0085 ,  A61K9/4816 ,  A61K9/4833 ,  A61M31/002 ,  B01J13/02 ,  B01J13/04 ,  C12N11/04 ,  C12N11/08 ,  C12N5/0012 ,  C12N5/0614 ,  C12N5/0619 ,  A61K2035/126 ,  A61K2035/128 ,  A61M2210/0687 ,  A61M2210/0693 ,  C12N2533/30

Abstract: Living cells such as animal cells which produce biologically active factors are encapsulated within a semipermeable, polymeric membrane such as polyacrylate by co-extruding an aqueous cell suspension and a polymeric solution through a common port having at least one concentric bores to form a tubular extrudate having a polymeric membrane which encapsulates the cell suspension. The cell suspension is extruded through an inner bore and the polymeric solution is extruded through an outer bore while a pressure differential is maintained between the cell suspension and the polymeric solution to impede solvent diffusion from the polymeric solution into the cell suspension. The polymeric solution coagulates to form an outer coating or membrane as the polymeric solution and the cell suspension are extruded through the extrusion port. As the outer membrane is formed, the ends of the tubular extrudate are sealed to form a cell capsule. In one embodiment, the tubular extrudate is sealed at intervals to define separate cell compartments connected by polymeric links. In another embodiment, a cell capsule connected to a tethering filament is formed. The polymeric membrane may contain additives such as a surfactant, an anti-inflammatory agent or an anti-oxidant and can be coated with a protective barrier. The cell suspension may contain nutrients and an anchorage substrate.

Abstract translation: 活性细胞如产生生物活性因子的动物细胞被包封在半透性聚合物膜（例如聚丙烯酸酯）内，通过共混挤出水性细胞悬浮液和聚合物溶液通过具有至少一个同心孔的共同孔口形成管状挤出物， 封装细胞悬浮液的聚合物膜。 细胞悬浮液通过内孔挤出，并且聚合物溶液通过外孔挤出，同时在细胞悬浮液和聚合物溶液之间保持压力差以阻止溶剂从聚合物溶液扩散进入细胞悬液。 聚合物溶液凝聚形成外涂层或膜作为聚合物溶液，并且细胞悬浮液通过挤出端口挤出。 当外膜形成时，管状挤出物的端部被密封以形成细胞囊。 在一个实施方案中，管状挤出物间隔密封以限定通过聚合物连接物连接的分离的细胞室。 在另一个实施例中，形成连接到系链细丝的细胞囊。 聚合物膜可以含有添加剂如表面活性剂，抗炎剂或抗氧化剂，并且可以用保护屏障涂覆。 细胞悬浮液可以含有营养物和锚定基质。

公开(公告)号：US5182111A

公开(公告)日：1993-01-26

申请号：US722894

申请日：1991-06-28

Applicant: Patrick Aebischer ,  Shelley R. Winn

Inventor： Patrick Aebischer ,  Shelley R. Winn

IPC: A61K9/52 ,  A61F2/02 ,  A61K9/00 ,  A61K35/12 ,  A61K35/14 ,  A61K35/22 ,  A61K35/28 ,  A61K35/30 ,  A61K35/39 ,  A61K38/00 ,  A61K38/22 ,  A61M31/00 ,  B01J13/02 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0793 ,  C12N11/00 ,  C12N11/04 ,  C12P1/00 ,  C12R1/91

CPC classification number: A61F2/022 ,  A61K35/22 ,  A61K35/30 ,  A61K38/185 ,  A61K9/0024 ,  A61K9/0085 ,  A61K9/0092 ,  A61M31/002 ,  B01J13/02 ,  C12N11/04 ,  C12N5/0012 ,  C12N5/0614 ,  C12N5/0619 ,  C12N5/0676 ,  C12P1/00 ,  A61M2210/0687 ,  A61M2210/0693 ,  C12N2533/30 ,  C12N2533/74

Abstract: Methods and devices are disclosed for the delivery of an active factor from an implanted co-culture of an active factor-secreting cell obtained from a first source and an augmentary substance-secreting cell obtained from a second source different from the first source, to a target region in a subject. The co-culture is maintained within a biocompatible, semipermeable membrane in which the augmentary substance secreted by the augmentary substance-producing cells stimulates the active factor-producing cells to secrete active factor. The semipermeable membrane permits the diffusion of the active factor therethrough while excluding detrimental agents present in the extenral environment from gaining access to the co-culture.

Abstract translation: 公开了用于从从第一来源获得的活性因子分泌细胞和从不同于第一来源的第二源获得的增生物质分泌细胞的植入共培养物递送活性因子的方法和装置， 受试者的目标区域。 共培养保持在生物相容的半透膜中，其中由增生物质产生细胞分泌的增殖物质刺激活性因子产生细胞分泌活性因子。 半透膜允许活性因子的扩散通过，同时排除存在于极端环境中的有害剂以获得共培养物质。

公开(公告)号：US5156844A

公开(公告)日：1992-10-20

申请号：US841875

申请日：1992-02-26

Applicant: Patrick Aebischer ,  Shelley R. Winn

Inventor： Patrick Aebischer ,  Shelley R. Winn

IPC: A61F2/02 ,  A61K9/00 ,  A61K35/12 ,  A61M31/00 ,  B01J13/02 ,  C12N5/00 ,  C12N5/071 ,  C12N5/0793 ,  C12N11/04

CPC classification number: A61K9/0092 ,  A61F2/022 ,  A61K35/12 ,  A61K9/0024 ,  A61K9/0085 ,  A61M31/002 ,  B01J13/02 ,  C12N11/04 ,  C12N5/0012 ,  C12N5/0614 ,  C12N5/0619 ,  C12N5/0676 ,  A61K2035/126 ,  A61M2210/0687 ,  A61M2210/0693 ,  C12N2533/30

Abstract: Neurological therapy devices are disclosed for the local and controlled delivery of a neurotransmitter to the brain of a subject suffering from neurotransmitter deficiency or dysfunction. In one embodiment the device includes a biocompatible, implantable, and retrievable polymeric insert including a source of neurotransmitter embedded therein. In another embodiment, the device includes a retrievable source of neurotransmitter including at least one neurotransmitter-secreting cell encapsulated within a semipermeable membrane allowing the diffusion therethrough of the neurotransmitter, and further includes a source of growth factor in close proximity to the neurotransmitter-secreting cells.

Abstract translation: 公开了用于局部和控制递送神经递质给患有神经递质缺乏或功能障碍的受试者脑部的神经治疗装置。 在一个实施方案中，该装置包括生物相容的，可植入的和可回收的聚合物插入物，其包含嵌入其中的神经递质源。 在另一个实施方案中，该装置包括可回收的神经递质来源，其包括至少一种包封在半透膜内的神经递质分泌细胞，其允许神经递质的扩散，并且还包括紧邻神经递质分泌细胞的生长因子源 。