利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

85 条记录 (耗时 0.028 秒)

    Capillary electrophoresis technique
    51.
    发明授权
    Capillary electrophoresis technique 失效
    毛细管电泳技术

    公开(公告)号：US5354440A

    公开(公告)日：1994-10-11

    申请号：US277566

    申请日：1988-11-29

    申请人： Robert W. Allington

    发明人： Robert W. Allington

    IPC分类号： G01N27/447 ,  G01N27/26

    CPC分类号： G01N27/44782 ,  G01N27/44721 ,  G01N27/44743

    摘要： To detect bands in an electrophoresis capillary tube having a liquid separating medium, a light source and a light detector of a monitor are positioned on opposite sides of the capillary tube with the light source transmitting light through a first slit and light passing to the detector through a second slit after it has passed through the capillary tube. The first and second slits are aligned with the direction of motion of bands, have a maximum length of less than 500 micrometers and a maximum width of less than 200 micrometers. There are no vertical lengths in the capillary tube having a dimension greater than one third of its length between a sample injecting end and a detecting end of the capillary tube. A sample injector includes a vacuum chamber communicating with one end of the capillary tube to pull sample into the other end and a pressure sensor to measure the pressure in said vacuum chamber and generate a signal indicative thereof and integrate it to indicate the amount of sample pulled into the system.

    摘要翻译： 为了检测具有液体分离介质的电泳毛细管中的条带，将监测器的光源和光检测器定位在毛细管的相对侧上，其中光源透过第一狭缝透射光并将光通过检测器通过 在其通过毛细管之后的第二狭缝。 第一和第二狭缝与带的运动方向对准，具有小于500微米的最大长度和小于200微米的最大宽度。 在毛细管中没有垂直长度，其长度大于样品注射端和毛细管的检测端之间的长度的三分之一。 样品注射器包括与毛细管的一端连通以将样品拉入另一端的真空室和用于测量所述真空室中的压力的​​压力传感器，并产生指示其的信号并将其整合以指示拉出的样品量 进入系统。

    Pulsed field gel electrophoresis of large DNA
    52.
    发明授权
    Pulsed field gel electrophoresis of large DNA 失效
    大DNA的脉冲场凝胶电泳

    公开(公告)号：US5336383A

    公开(公告)日：1994-08-09

    申请号：US495899

    申请日：1990-03-15

    申请人： Robert W. Allington

    发明人： Robert W. Allington

    IPC分类号： G01N33/50 ,  G01N27/447 ,  G01N27/26

    CPC分类号： G01N27/44773

    摘要： To separate high-molecular DNA strands, an electrophoresis system includes first means for changing the direction of an electric field within an electrophoresis separating unit at a first frequency having a period of no more than 20 seconds and second means for changing the electric field in another manner at a second frequency at least twice as high as the said first frequency. In one embodiment, the field strength in the direction of overall migration is selected so that, considering the DNA size and gel density, the leading segment of DNA to the first corner is sufficiently small so that excess friction forces due to bunching does not cause excessive band spreading. The field strength is selected so that the electric field is reduced until the changing of the electric field at the second frequency keeps the bunching effect to a sufficiently low level to not seriously interfere with the separation.

    摘要翻译： 为了分离高分子DNA链，电泳系统包括用于以不超过20秒的周期改变电泳分离单元内的电场方向的第一装置和用于改变另一个的电场的第二装置 方式在第二频率下至少是所述第一频率的两倍。 在一个实施方案中，选择总体迁移方向的场强，使得考虑到DNA尺寸和凝胶密度，到第一角的DNA的前导部分足够小，使得由于聚束引起的过量摩擦力不会引起过量 频段扩展。 选择场强，使得电场减小，直到第二频率的电场的变化将聚束效应保持在足够低的水平，以不严重干扰分离。

    Chromatographic pumping method
    56.
    发明授权
    Chromatographic pumping method 失效
    色谱抽取法

    公开(公告)号：US4986919A

    公开(公告)日：1991-01-22

    申请号：US355683

    申请日：1989-05-22

    申请人： Robert W. Allington

    发明人： Robert W. Allington

    IPC分类号： F04B49/06 ,  G01N30/32

    CPC分类号： G01N30/32 ,  F04B49/065 ,  G01N2030/326

    摘要： To provide smooth constant flow from a pump, a chromatographic system comprises: a chromatographic column having an inlet; a pump for supplying fluid to the inlet of the chromatographic column; a power means for the pump motor; positive and negative feedback loop means for controlling said power means; means for energizing and de-energizing said positive and negative feedback control means; said negative feedback control means receiving a signal from said means for measuring flow rate and including means for comparing said signal with said corrected flow rate reference signal while said second feedback loop is energized to generate an error signal controlling said power means; and said positive feedback control means applying an acceleration voltage to said motor from a time a preset period after the initiation of a return stroke of said piston until after a timed duration.

    摘要翻译： 为了从泵提供平稳的恒定流量，色谱系统包括：具有入口的色谱柱; 用于将液体供应到色谱柱的入口的泵; 用于泵马达的动力装置; 用于控制所述功率装置的正和负反馈回路装置; 用于对所述正和负反馈控制装置通电和断电的装置; 所述负反馈控制装置接收来自所述用于测量流量的装置的信号，并且包括用于在所述第二反馈回路通电时将所述信号与所述校正的流量参考信号进行比较的装置，以产生控制所述功率装置的误差信号; 并且所述正反馈控制装置从所述活塞的返回冲程开始到定时持续时间之后的预定时段的时间向所述电动机施加加速电压。

    Gradient system
    57.
    发明授权
    Gradient system 失效
    渐变系统

    公开(公告)号：US4981597A

    公开(公告)日：1991-01-01

    申请号：US355881

    申请日：1989-05-19

    申请人： Robert W. Allington ,  Daniel G. Jameson

    发明人： Robert W. Allington ,  Daniel G. Jameson

    IPC分类号： G01N30/34

    CPC分类号： G01N30/34 ,  G01N2030/347

    摘要： To supply a programmed gradient to a high pressure pump for introduction into a column, a mixer, degasser and accumulator communicates with the high pressure pump to supply fluid thereto, having a volume no more than 150 times the chamber volume of the high pressure pump. The mixer, degasser and accumulator includes temperature sensors which sense when the mixer, degasser and accumulator has been emptied to a level where it cannot respond to a demand for fluid from said high pressure pump and provides a signal to a low pressure pump which responds by cycling to again fill the mixer, degasser and accumulator. Upon receiving a demand signal, the low pressure pump fills by drawing fluid from a plurality of fluid sources to compose the gradient being used at that time, with the pump slowing during valve opening and closing so as to avoid cavitation.

    摘要翻译： 为了向高压泵提供编程梯度以引入塔中，混合器，脱气器和蓄能器与高压泵连通以向其提供流体，其体积不超过高压泵的室容积的150倍。 混合器，脱气机和蓄能器包括温度传感器，其感测混合器，脱气器和蓄能器何时被排空到不能响应来自所述高压泵的流体需求的水平，并且向低压泵提供信号，该低压泵由 循环再次填充混合器，脱气机和蓄压器。 在接收到需求信号时，通过从多个流体源抽取流体来填充低压泵以构成当时使用的梯度，同时在阀打开和关闭期间泵减慢，以避免空化。

    Apparatus for reducing tailing in a liquid chromatograph
    58.
    发明授权
    Apparatus for reducing tailing in a liquid chromatograph 失效
    用于在液相色谱仪中减少拖尾的装置

    公开(公告)号：US4863592A

    公开(公告)日：1989-09-05

    申请号：US117371

    申请日：1987-10-28

    申请人： Robert W. Allington ,  Abolghassem Y. Tehrani ,  John N. Jones

    发明人： Robert W. Allington ,  Abolghassem Y. Tehrani ,  John N. Jones

    IPC分类号： B01D15/08 ,  G01N30/60 ,  G01N30/74

    CPC分类号： G01N30/603 ,  B01D15/08 ,  G01N30/6017 ,  G01N30/6026 ,  G01N30/6052 ,  G01N30/74

    摘要： To reduce tailing, the frit at the outlet to the liquid chromatographic column has a diameter substantially equal to that of the inside diameter of the outlet end of the column and less than two millimeters. It is held directly against the packing of the column within a cylindrical member having a shoulder with a central aperture at least the size of the diameter of the frit and cylindrical walls which are fastened to resist force in the direction of the axis of the column from its inlet to its outlet. A gasket seals the cylindrical holder against the column wall. The frit is formed of gold-plated nickel spheres sintered together at a temperature lower than the gold-nickel eutectic temperature of 950 degrees Celsius but above the phase-immiscibility temperature of 720 degrees Celsius. The size and shape of the frit is selected to reduce tailing by causing the flow path from the column to be uniform.

    摘要翻译： 为了减少拖尾，液相色谱柱出口处的玻璃料的直径基本上等于柱的出口端的内径，直径小于2毫米。 它被直接抵靠在具有肩部的圆柱形构件的圆柱形构件中，所述圆柱形构件具有至少具有中心孔的至少大小的玻璃料和圆柱形壁的直径的尺寸，所述玻璃料和圆柱形壁被紧固以抵抗在柱的轴线方向上的力 其入口到其出口。 垫圈将柱形保持器密封在柱壁上。 玻璃料由在950℃以下的金 - 镍共晶温度下高于720℃的相互不混溶温度的温度下烧结在一起的镀金镍球形成。 选择玻璃料的尺寸和形状以通过使来自塔的流动路径均匀来减少拖尾。

    Apparatus for liquid chromatography
    59.
    发明授权
    Apparatus for liquid chromatography 失效
    液相色谱装置

    公开(公告)号：US4772388A

    公开(公告)日：1988-09-20

    申请号：US415471

    申请日：1982-09-07

    申请人： Robert W. Allington

    发明人： Robert W. Allington

    IPC分类号： G01N30/32 ,  B01D15/08

    CPC分类号： G01N30/32 ,  G01N2030/326

    摘要： To stabilize flow rate in a single-stroke, syringe-type high pressure pump to a constant value after turn-on, a control system increases the pump speed until equilibrium conditions are reached and then pumps at the preset rate. In one embodiment, the control system senses the maximum rate of change of pressure, detects a pressure when the rate of change is two-thirds of the maximum, increases the pump rate until the pressure is three times that at the value of the detected pressure and begins pumping at the preset constant flow rate.In other embodiments: (1) a pumping system is operated at a flow rate ten times the preset flow rate and, when the time derivative of the pressure has dropped to nine tenths of its maximum, the pump motor speed is reduced to the preset flow rate; (2) the outflow of the column is measured under no load conditions at the desired flow rate and when a column is connected, the pumping speed is increased until the same outflow results before reducing the pumping rate, and (3) the system is operated in a constant pressure mode to a point at which the pressure is estimated to provide the desired flow rate, the flow rate measured, and if the flow rate is not the preset one, a new pressure measurement is set and the procedure repeated.

    摘要翻译： 为了将单冲程注射器式高压泵的流量稳定在恒定值，启动后，控制系统会提高泵的转速，直到达到平衡条件，然后以预设的速率泵送。 在一个实施例中，控制系统感测压力的最大变化率，当变化率是最大值的三分之二时检测压力，增加泵速，直到压力是检测压力值的三倍 并以预设的恒定流速开始泵送。 在其他实施例中：（1）泵送系统以预设流量的十倍的流速操作，并且当压力的时间导数已经下降到其最大值的十分之一时，泵电机速度降低到预设流量 率; （2）在无负载条件下以期望的流量测量柱的流出，并且当连接柱时，泵送速度增加，直到在降低泵送速率之前产生相同的流出，并且（3）系统被操作 在恒定压力模式下，估计压力以提供所需流量，测量流量，如果流量不是预设流量，则设置新的压力测量并重复该过程。