公开(公告)号：US20150151307A1

公开(公告)日：2015-06-04

申请号：US14520510

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Keith J. Breinlinger ,  Daniele Malleo ,  Gaetan L. Mathieu ,  J. Tanner Nevill ,  Alexander H. Slocum ,  Mark P. White

IPC: B03C7/02 ,  B81B7/02

CPC classification number: B81B7/02 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C7/023 ,  B03C2201/26 ,  C12M23/16 ,  C12M41/46 ,  C12M47/02 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/5023 ,  G01N33/543 ,  G01N33/54326 ,  G01N35/10

Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.

Abstract translation: 微流体装置中的保持笔中的一组微物体可以被选择并移动到分段区域，微物体可以从微流体装置输出。 微流体装置可以具有多个保持笔，并且每个保持笔可以将位于保持笔中的微物体与位于另一个保持笔中的微物体或微流体装置中的其他地方隔离。 所选择的一组微物体可以包含一个或多个生物细胞，例如克隆的细胞群。 因此，本发明的实施方案可以在微流体装置中选择特定的一组克隆细胞，将克隆细胞移动到分期区域，并从微流体装置输出克隆细胞，同时保持输出组的克隆性质。

公开(公告)号：US20150151298A1

公开(公告)日：2015-06-04

申请号：US14520568

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Eric D. Hobbs ,  Mark P. White ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00 ,  G01N33/558

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  G01N33/558 ,  G01N2469/20

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

Abstract translation: 微流体装置可以包括至少一个流体连接到未被吹扫的区域的扫掠区域。 扫掠区域和未被扫描的区域之间的流体连接可以实现扩散，但是基本上不会在扫掠区域和未被扫描区域之间的介质流动。 可以在这种微流体装置中测定生物微生物产生感兴趣分析物的能力。 可以选择装载到微流体装置中的样品材料中的生物微物体用于特定特征并且被置于未被扫描的区域中。 样品材料然后可以从扫掠区域流出，并且测定材料流入扫掠区域。 扫描区域中介质的流动基本上不影响未被扫描的区域中的生物微物体，但是由生物微物体产生的任何目标分析物可以从未被扫描的区域扩散到扫描区域，其中分析物可与 测定材料以产生局部可检测的反应。 可以分析任何这样的检测到的反应，以确定生物微物体（如果有的话）是感兴趣的分析物的生产者。