公开(公告)号：US20080223865A1

公开(公告)日：2008-09-18

申请号：US11994590

申请日：2006-06-27

申请人： Andreas Althof ,  Stefan Fuehling ,  Christian Krogull ,  Knut Meyer ,  Frank Reiter

发明人： Andreas Althof ,  Stefan Fuehling ,  Christian Krogull ,  Knut Meyer ,  Frank Reiter

IPC分类号： B60K15/035 ,  F02M37/04

CPC分类号： F02M37/106 ,  B60K15/03504 ,  B60K15/077 ,  B60K2015/03111 ,  B60K2015/03467 ,  B60K2015/03509 ,  B60K2015/03557

摘要： A device for delivering fuel has a fuel delivery unit placed inside a fuel tank and which consists of a swirl pot with a fuel pump situated therein for delivering fuel out via a flow line to an internal combustion engine, and with a ventilation system located inside the fuel tank and serves to withdraw fuel vapors from the fuel tank. This ventilation system consists of ventilation lines and a liquid trap for precipitating fuel. The ventilation lines are arranged with one end in the upper area of the fuel tank, and, with the other end, lead into the liquid trap. An activated charcoal filter located downstream from the liquid trap has a connection to the atmosphere. The liquid trap and the activated charcoal filter are situated next and at the height of the swirl pot. At least the liquid trap is mechanically connected to the swirl pot.

摘要翻译： 用于输送燃料的装置具有放置在燃料箱内部的燃料输送单元，该燃料输送单元由具有位于其中的燃料泵的涡流罐组成，用于经由流动管线将燃料输出到内燃机，以及位于内部的通风系统 燃料箱，用于从燃料箱中取出燃料蒸汽。 该通风系统由通风管道和用于沉淀燃料的液体捕集器组成。 通风管一端设置在燃油箱的上部区域，另一端引入液阱。 位于液阱的下游的活性炭过滤器与大气连接。 液体捕集器和活性炭过滤器位于涡流锅的下一个处和高度处。 至少液体捕集器机械地连接到涡流罐。

公开(公告)号：US07361811B2

公开(公告)日：2008-04-22

申请号：US10718311

申请日：2003-11-20

申请人： Knut Meyer ,  Paul V. Viitanen ,  Drew E. Van Dyk

发明人： Knut Meyer ,  Paul V. Viitanen ,  Drew E. Van Dyk

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/88 ,  C07K2319/00 ,  C12N15/8243

摘要： The invention relates to high-level production of pHBA in green plants using a unique expression cassette. The latter comprises a chorismate pyruvate lyase (CPL) coding sequence operably linked to a suitable promoter capable of driving protein expression in higher plants. Additionally, the CPL cassette comprises a sequence encoding a chloroplast transit peptide, its natural cleavage site, and a small portion of the transit peptide donor protein fused to the N-terminus of CPL. The chloroplast targeting sequence targets the foreign protein to the chloroplast compartment and aids in its uptake into the organelle. The cleavage site is unique to the transit peptide, and cleavage of the chimeric protein encoded by the cassette at this site releases a novel polypeptide that has full enzyme activity, comprising the mature CPL enzyme and a small portion of the transit peptide donor.

摘要翻译： 本发明涉及使用独特的表达盒在绿色植物中高水平生产pHBA。 后者包括可操作地连接到能够驱动高等植物中的蛋白质表达的合适的启动子的草酸丙酸裂解酶（CPL）编码序列。 此外，CPL盒包含编码叶绿体转运肽，其天然切割位点的序列和与CPL的N末端融合的转运肽供体蛋白的一小部分。 叶绿体靶向序列将外来蛋白质靶向叶绿体隔室，并有助于其吸收到细胞器中。 切割位点对于转运肽是独特的，并且在该位点处由盒编码的嵌合蛋白质的切割释放出具有完整酶活性的新型多肽，其包含成熟的CPL酶和少量的转运肽供体。

公开(公告)号：US20070184092A1

公开(公告)日：2007-08-09

申请号：US11656047

申请日：2007-01-22

申请人： Knut Meyer ,  Bo Shen ,  Mitchell Tarczynski

发明人： Knut Meyer ,  Bo Shen ,  Mitchell Tarczynski

IPC分类号： A01H5/00 ,  A61K47/00

CPC分类号： C12N15/8247 ,  A61K9/0024 ,  A61K9/0043 ,  C12N15/8243 ,  C12N15/8271

摘要： Compositions comprising a modulated tocol content in a plant or plant part are provided. In specific embodiments, the compositions and methods of the invention modulate tocol content by modulating the level of a polypeptide having a LEC1-type B domain in combination with modulating the level of at least one other polypeptide involved in tocol biosynthesis. Plants, plant parts, grain, seed and oil having the modulated tocol level are also provided. Methods to enhance oxidative stress tolerance of a plant or plant part, increase shelf-life, enhance the nutritional value, and improve tissue quality are also provided.

摘要翻译： 提供了包含植物或植物部分中调制的多克隆内容物的组合物。 在具体实施方案中，本发明的组合物和方法通过调节具有LEC1型B结构域的多肽的水平并结合调节参与多巴胺生物合成的至少一种其他多肽的水平来调节多克隆含量。 还提供了植物，植物部分，具有调制多酚水平的谷物，种子和油。 还提供了提高植物或植物部分的氧化应激耐受性，增加保质期，增强营养价值和改善组织质量的方法。

公开(公告)号：US06209594B1

公开(公告)日：2001-04-03

申请号：US09370495

申请日：1999-08-09

申请人： Knut Meyer ,  Peter Andreas Löw

发明人： Knut Meyer ,  Peter Andreas Löw

IPC分类号： B65B104

CPC分类号： B60K15/077 ,  B60K15/04 ,  Y10T137/469 ,  Y10T137/479 ,  Y10T137/4824

摘要： In the case of a filling pipe for a fuel tank a branching element is arranged in a section of the filling pipe. The flow of the inflowing fuel preferably runs along an outer wall of the filling pipe, so that a subflow is in each case conducted directly to the antisurge tower through a lower cross-sectional area. Larger volume flows, which completely fill the cross section, pass for the most part directly into the fuel tank. By this means, a sufficient basic supply of the antisurge tower is ensured irrespective of the volume flow produced during filling.

摘要翻译： 在用于燃料箱的填充管的情况下，分配元件布置在填充管的一部分中。 流入燃料的流动优选地沿着填充管的外壁流动，使得子流在每种情况下通过较低的横截面积直接传导到防喘振塔。 更大的体积流量，其完全填充横截面，大部分通过直接进入燃料箱。 通过这种方式，无论在填充过程中产生的体积流量如何，都能确保足够的基础供应的防喘振塔。

公开(公告)号：US08927809B2

公开(公告)日：2015-01-06

申请号：US13295345

申请日：2011-11-14

申请人： Knut Meyer ,  William D. Hitz ,  Narendra S. Yadav ,  Howard Glenn Damude

发明人： Knut Meyer ,  William D. Hitz ,  Narendra S. Yadav ,  Howard Glenn Damude

IPC分类号： A01H5/00 ,  A01H4/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/54 ,  C12P21/00 ,  C12P7/64 ,  A23L1/20

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12Y203/01158

摘要： Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia Lipolytica are used to achieve the increase in seed storage lipids.

摘要翻译： 描述了与非转基因，无偏分离大豆种子的总脂肪酸含量相比，具有至少10％的总脂肪酸含量增加和改变的脂肪酸分布的转基因大豆种子。 来自解脂耶氏酵母的DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US08293976B2

公开(公告)日：2012-10-23

申请号：US13005369

申请日：2011-01-12

申请人： Knut Meyer

发明人： Knut Meyer

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC分类号： C12N15/79 ,  A61K31/355 ,  C12N9/1007 ,  C12N15/8243

摘要： Preparation and use of isolated nucleic acids useful in altering the oil phenotype of plants are described. Isolated nucleic acids and their encoded polypeptides are described that alter the content of alpha-tocotrienol, beta-tocotrienol, or both, in transformed seeds and oil obtained from the transformed seeds. Expression cassettes, host cells and transformed plants are described that contain the foregoing nucleic acids.

摘要翻译： 描述了可用于改变植物的油表型的分离的核酸的制备和使用。 描述了分离的核酸及其编码的多肽，其在转化的种子和从转化的种子获得的油中改变α-生育三烯酚，β-生育三烯酚或二者的含量。 描述了含有前述核酸的表达盒，宿主细胞和转化植物。

公开(公告)号：US20110219474A1

公开(公告)日：2011-09-08

申请号：US13039779

申请日：2011-03-03

申请人： Knut Meyer ,  Kevin L. Stecca

发明人： Knut Meyer ,  Kevin L. Stecca

IPC分类号： C12N15/87 ,  A01H5/00 ,  C07H21/04

CPC分类号： C12N15/8251 ,  C12N5/14 ,  C12N9/0004 ,  C12N15/8245 ,  C12N15/8247

摘要： This invention is in the field of plant molecular biology. More specifically, this invention pertains to isolated nucleic acid fragments encoding ORM proteins in plants and seeds and the use of such fragments to modulate expression of a gene encoding ORM protein activity in a transformed host cell.

摘要翻译： 本发明在植物分子生物学领域。 更具体地，本发明涉及在植物和种子中编码ORM蛋白的分离的核酸片段，以及这样的片段在转化的宿主细胞中调节编码ORM蛋白活性的基因的表达的用途。

公开(公告)号：US20110126318A1

公开(公告)日：2011-05-26

申请号：US12917024

申请日：2010-11-01

申请人： Stephen M. Allen ,  Howard Glenn Damude ,  John D. Everard ,  Knut Meyer ,  Byung-Chun Yoo

发明人： Stephen M. Allen ,  Howard Glenn Damude ,  John D. Everard ,  Knut Meyer ,  Byung-Chun Yoo

IPC分类号： A01H5/10 ,  A01H5/00 ,  C12N15/82 ,  C07H21/04 ,  C07K14/415 ,  C12Q1/68 ,  C12N9/10

CPC分类号： C12N15/8247 ,  C07K14/415 ,  C12N9/1029 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/16 ,  C12Y203/0102

摘要： This invention relates to polynucleotide sequences encoding SUT2 or SUT4 sucrose transporter genes. Methods for increasing seed oil content and evaluating increased oil content in a plant seed are described. The compositions and methods disclosed herein employ a variety of sequences that encode sucrose transporters and a variety of sequences that influence fatty acid accumulation, including for example, DGAT, Lec1 and ODP1 transcription factor. In specific embodiments, overexpression of SUT2 and/or SUT4 sucrose transporters in combination with DGAT genes further increase plant seed oil production compared to a high oil plant comprising recombinant DNA constructs that do not overexpress SUT2 or SUT4 transporters.

摘要翻译： 本发明涉及编码SUT2或SUT4蔗糖转运蛋白基因的多核苷酸序列。 描述了增加种子油含量和评估植物种子中增加的油含量的方法。 本文公开的组合物和方法使用编码蔗糖转运蛋白的多种序列和影响脂肪酸积累的多种序列，包括例如DGAT，Lec1和ODP1转录因子。 在具体实施方案中，与包含不过表达SUT2或SUT4转运蛋白的重组DNA构建体的高油植物相比，SUT2和/或SUT4蔗糖转运蛋白与DGAT基因的过表达进一步增加植物种子油产量。

公开(公告)号：US20110113503A1

公开(公告)日：2011-05-12

申请号：US13005369

申请日：2011-01-12

申请人： Knut Meyer

发明人： Knut Meyer

IPC分类号： C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/79 ,  A61K31/355 ,  C12N9/1007 ,  C12N15/8243

摘要： Preparation and use of isolated nucleic acids useful in altering the oil phenotype of plants are described. Isolated nucleic acids and their encoded polypeptides are described that alter the content of alpha-tocotrienol, beta-tocotrienol, or both, in transformed seeds and oil obtained from the transformed seeds. Expression cassettes, host cells and transformed plants are described that contain the foregoing nucleic acids.

摘要翻译： 描述了可用于改变植物的油表型的分离的核酸的制备和使用。 描述了分离的核酸及其编码的多肽，其在转化的种子和从转化的种子获得的油中改变α-生育三烯酚，β-生育三烯酚或二者的含量。 描述了含有前述核酸的表达盒，宿主细胞和转化植物。

公开(公告)号：US20080313770A1

公开(公告)日：2008-12-18

申请号：US11655654

申请日：2007-01-19

申请人： Knut Meyer ,  Bo Shen ,  Mitchell C. Tarczynski

发明人： Knut Meyer ,  Bo Shen ,  Mitchell C. Tarczynski

IPC分类号： C12N15/29 ,  A01H5/00

CPC分类号： C12N15/8247 ,  A61K9/0024 ,  A61K9/0043 ,  C12N15/8243 ,  C12N15/8271

摘要： Compositions comprising a modulated tocol content in a plant or plant part are provided. In specific embodiments, the compositions and methods of the invention modulate tocol content by modulating the level of a polypeptide having a LEC1-type B domain in combination with modulating the level of at least one other polypeptide involved in tocol biosynthesis. Plants, plant parts, grain, seed and oil having the modulated tocol level are also provided. Methods to enhance oxidative stress tolerance of a plant or plant part, increase shelf-life, enhance the nutritional value, and improve tissue quality are also provided.

摘要翻译： 提供了包含植物或植物部分中调制的多克隆内容物的组合物。 在具体实施方案中，本发明的组合物和方法通过调节具有LEC1型B结构域的多肽的水平并结合调节参与多巴胺生物合成的至少一种其他多肽的水平来调节多克隆含量。 还提供了植物，植物部分，具有调制多酚水平的谷物，种子和油。 还提供了增强植物或植物部分的氧化应激耐受性，增加保质期，增强营养价值和改善组织质量的方法。