公开(公告)号：US20210403849A1

公开(公告)日：2021-12-30

申请号：US16606742

申请日：2018-04-27

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Daixi Xin ,  Yang Lei

IPC: C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  C12N5/00 ,  C12M3/06

Abstract: A cellular object imaging system may include a growth platform. The growth platform may include a body having a cellular fluid suspension region, a media supply passage extending within the body to the cellular object fluid suspension region, at least one fluid pump on the body to selectively deliver media to the cellular object fluid suspension region, a waste discharge passage extending within the body from the cellular object fluid suspension region and a cellular object rotator on the body adjacent the cellular object fluid suspension region to rotate a cellular object within the cellular object fluid suspension region. The cellular object fluid suspension region permits optical imaging of the cellular object suspended in a fluid during rotation by the cellular object rotator.

公开(公告)号：US20210346885A1

公开(公告)日：2021-11-11

申请号：US17059650

申请日：2019-02-01

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov ,  Diane R. Hammerstad

IPC: B01L3/00 ,  G01N15/00

Abstract: In one example in accordance with the present disclosure, a cellular analytic system is described. The cellular analytic system includes an analytic device. The analytic device includes a chamber to receive a cell to be analyzed. At least one lysing element agitates the cell and at least one sensor detects a change in the cell based on an agitation of the cell. The cellular analytic system also includes a controller to determine a rupture threshold of the cell based on parameters of the agitation when a cell membrane ruptures.

公开(公告)号：US20210299662A1

公开(公告)日：2021-09-30

申请号：US16605619

申请日：2017-10-30

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua Chen ,  Michael W Cumbie

IPC: B01L3/00 ,  G01N15/14

Abstract: In an example implementation, a microfluidic device includes a first layer with a first microfluidic channel and a second layer with a second microfluidic channel. The first and second channels are adjacent to one another at a channel intersection, and a conductive membrane valve extends across and covers the channel intersection to separate the first and second channels. The microfluidic device includes a conductive trace to open the membrane valve and join the first and second channels by supplying an electric current to heat and melt a thinned region of the membrane valve.

公开(公告)号：US20210238537A1

公开(公告)日：2021-08-05

申请号：US17049406

申请日：2018-08-10

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander Govyadinov ,  Diane R. Hammerstad ,  Viktor Shkolnikov

IPC: C12M1/00 ,  C12M1/36 ,  C12N1/06

Abstract: In one example in accordance with the present disclosure, a conductivity-based lysis monitor is described. The lysis monitoring device includes a lysing chamber to receive a cell to be lysed and at least one lysing device to rupture a cell membrane. At least one pair of electrodes are disposed in the lysing chamber to detect a level of conductivity in the lysing chamber. A controller of the device determines when the cell membrane has ruptured based on detected levels of conductivity in the lysing chamber.

公开(公告)号：US20200224145A1

公开(公告)日：2020-07-16

申请号：US16605193

申请日：2017-09-23

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Diane R. Hammerstad ,  Michael W. Cumbie

IPC: C12M1/42 ,  C12M1/36 ,  C12N13/00 ,  C12N15/87 ,  C12M1/26

Abstract: An electroporation system may include a well plate, a dispenser and a dispenser-well positioning system. The well plate may include wells, each of the wells including an interior, a first electrode adjacent the interior and a second electrode adjacent the interior and spaced from the first electrode. The first electrode and the second electrode are to apply an electrostatic field across the well. The dispenser is to dispense a cell having a diameter into each of the wells. The dispenser-well positioning system is to align each well and the dispenser such that the dispenser dispenses the cell into each well at a location spaced from the first electrode and the second electrode by a distance of at least 5 times the diameter of the cell.

公开(公告)号：US20190154587A1

公开(公告)日：2019-05-23

申请号：US16098067

申请日：2016-07-20

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Ning Ge ,  Robert G. Walmsley ,  Helen A. Holder ,  Steven J. Simske ,  Anita Rogacs ,  Viktor Shkolnikov

IPC: G01N21/65

CPC classification number: G01N21/658 ,  B82Y15/00

Abstract: A gas conduit directs a flow of gas from a gas flow source. A surface enhanced luminescence (SEL) stage is within the conduit and includes a substrate and nano fingers projecting from the substrate. A heater heats the nano fingers to a temperature so as to soften the nano fingers such that the nano fingers collapse towards each other to capture molecules entrained in the gas therebetween.

公开(公告)号：US20190145897A1

公开(公告)日：2019-05-16

申请号：US16097962

申请日：2016-07-22

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Ning Ge ,  Helen A. Holder ,  Steven J. Simske ,  Anita Rogacs ,  Viktor Shkolnikov

IPC: G01N21/65 ,  G01J3/44

Abstract: An activatable SEL sensor stage may include a substrate, cation metal-based material masses supported by the substrate and isolated from one another and dielectric capping layer over the cation metal-based material masses to inhibit oxidation of the cation metal-based material masses.

公开(公告)号：US20240377324A1

公开(公告)日：2024-11-14

申请号：US18564531

申请日：2021-05-28

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov ,  Fausto D'Apuzzo

IPC: G01N21/64

Abstract: In one example in accordance with the present disclosure, a fluorescence detection system is described. The fluorescence detection system includes a microfluidic chamber to receive a sample containing a compound to be detected. An illumination system provides an excitation light to excite fluorophores in the microfluidic chamber. An outcoupler is disposed between the illumination system and the microfluidic chamber to diffuse the excitation light to fill the microfluidic chamber. The fluorescence detection system also includes a detection system to detect fluorescence generated by the excitation of the fluorophores in the microfluidic chamber.

公开(公告)号：US20240201130A1

公开(公告)日：2024-06-20

申请号：US18590723

申请日：2024-02-28

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Yang Lei ,  Daixi Xin

IPC: G01N27/447 ,  B03C5/00 ,  B03C5/02

CPC classification number: G01N27/44713 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: In example implementations, an apparatus is provided. The apparatus includes a dielectrophoresis (DEP) separator, an electrical field generator, a tracking system, and a controller. The DEP separator is to separate a plurality of different particles. The electrical field generator is coupled to the DEP separator to apply a frequency to the DEP separator. The tracking system is to track a movement of a type of particles in the DEP separator. The controller is in communication with the electrical field generator to control the frequency and the tracking system to track the separation. The controller is to calculate a cross-over frequency from a cross-over frequency distribution for the type of particles based on a frequency sweep performed on the type of particles and the movement of the type of particles that is tracked.

公开(公告)号：US20240116055A1

公开(公告)日：2024-04-11

申请号：US17960942

申请日：2022-10-06

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Michael W. Cumbie ,  Chien-Hua Chen ,  Viktor Shkolnikov

IPC: B01L3/00 ,  C12M3/06

CPC classification number: B01L3/502792 ,  B01L3/502715 ,  B01L3/50273 ,  C12M23/16 ,  B01L2200/027 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0427

Abstract: An example digital microfluidic device can include a hydrophobic electrowetting surface including an array of electrodes. The individual electrodes can have a shape with three or more sides. The array of electrodes can include a parking electrode and an adjacent electrode that is adjacent to the parking electrode. A cover can be positioned over the electrowetting surface at a gap distance sufficient to accommodate a liquid droplet between the cover and the electrowetting surface. A plurality of droplet barriers can be positioned on three or more sides of the parking electrode. The droplet barriers can constrain movement of a liquid droplet from the parking electrode to the adjacent electrode.