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公开(公告)号：US08916691B2
公开(公告)日：2014-12-23
申请号：US11545349
申请日：2006-10-10
IPC分类号： C07K1/36 , C07K1/22 , C07K14/435 , C07K16/18 , C07K14/78 , A61L27/38 , C07K14/47 , A61K48/00 , A61K35/12 , A61K35/00
CPC分类号： A61L27/3856 , A61K35/12 , A61K35/13 , A61K48/00 , A61K2121/00 , C07K14/47 , C07K14/78 , C07K16/18 , C12N2799/022
摘要： Methods of expressing LIM mineralization protein in mammalian cells are described. Methods of expressing LIM mineralization protein and assessing glycosylation of the LIM mineralization protein in prokaryotic and non-mammalian eukaryotic cells are also described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein. Transfection may be accomplished in vitro, ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid.
摘要翻译： 描述了在哺乳动物细胞中表达LIM矿化蛋白的方法。 还描述了在原核和非哺乳动物真核细胞中表达LIM矿化蛋白和评估LIM矿化蛋白的糖基化的方法。 所述方法包括用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸转染细胞。 转染可以通过直接注射病毒或裸露DNA或非病毒载体如质粒在体外，离体或体内完成。
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52.发明授权公开(公告)号：US08470354B2
公开(公告)日：2013-06-25
申请号：US12684480
申请日：2010-01-08
申请人： William F. McKay , Scott D. Boden , Neil B. Beals
发明人： William F. McKay , Scott D. Boden , Neil B. Beals
IPC分类号： A61F2/00 , A61F2/02 , A61F2/28 , A61K38/00 , A61K38/16 , A61K38/17 , A61K38/18 , C07K14/78 , C07K14/51
CPC分类号： A61B17/707 , A61B17/8085 , A61B2017/00004 , A61K38/1875 , A61L27/227 , A61L27/24 , A61L27/54 , A61L27/56 , A61L27/58 , A61L2300/252 , A61L2300/414 , A61L2430/02 , C07K14/51 , C07K14/78 , Y10S514/801
摘要： Described are osteogenic implants that include a first implant material covered at least in part by a second implant material carrying an osteogenic protein such as a bone morphogenic protein. The first implant material can comprise a mineral and provide an inner scaffolding portion for supporting bone ingrowth, and the second implant material can comprise a collagen or other sponge carrier covering the first implant material and having a liquid osteogenic protein formulation imbibed therein. Related implant materials and methods of preparation and use constitute additional aspects of the invention.
摘要翻译： 描述的是成骨植入物，其包括至少部分地由承载成骨蛋白质例如骨形态发生蛋白质的第二种植体材料覆盖的第一种植体材料。 第一植入材料可以包括矿物并且提供用于支撑骨向内生长的内部支架部分，并且第二植入材料可以包括覆盖第一植入材料并且具有吸入其中的液体成骨蛋白质制剂的胶原蛋白或其它海绵载体。 相关植入材料及其制备和使用方法构成本发明的另外方面。
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公开(公告)号：US20130013071A1
公开(公告)日：2013-01-10
申请号：US13618599
申请日：2012-09-14
申请人： Randal R. Betz , Scott D. Boden , Christine Clark , Nanette Forsyth , John W. Morris , Kathy Traianedes
发明人： Randal R. Betz , Scott D. Boden , Christine Clark , Nanette Forsyth , John W. Morris , Kathy Traianedes
CPC分类号： A61F2/28 , A61F2/4455 , A61F2/4644 , A61F2002/2817 , A61F2002/2835 , A61F2002/30059 , A61F2002/30075 , A61F2002/30153 , A61F2002/30329 , A61F2002/30593 , A61F2002/30677 , A61F2002/30805 , A61F2002/30957 , A61F2002/30975 , A61F2002/4649 , A61F2210/0061 , A61F2220/0025 , A61F2230/0019 , A61F2310/00293 , A61F2310/00329 , A61F2310/00365 , A61L27/3608 , A61L27/365 , A61L27/3658 , A61L27/38 , A61L31/005 , A61L2430/02
摘要： An osteoimplant is made up of a coherent aggregate of elongate bone particles.
摘要翻译： 骨植入物由细长骨颗粒的相干骨料组成。
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公开(公告)号：US08148326B2
公开(公告)日：2012-04-03
申请号：US12635289
申请日：2009-12-10
CPC分类号： A61K9/0024 , A61K9/0085 , A61K38/177 , A61K38/1774 , A61K38/18 , A61K38/1825 , A61K38/1841 , A61K38/1858 , A61K38/1866 , A61K38/1875 , A61K38/19 , A61K38/30 , A61K47/42 , A61L27/24 , A61L27/54 , A61L27/56 , A61L2300/414
摘要： A carrier matrix may be delivered to a target position within a patient in a minimally invasive manner by first cutting a collagen sponge sheet into a plurality of relatively small pieces. These pieces are sized so that, when wet, they are capable of flowing through a cannula and/or reduced-diameter syringe tip. The pieces are placed into a syringe and wetted, say with a morphogenic solution, and optionally mixed with a bulking material, which is similarly sized to fit through the cannula. The thoroughly mixed and wetted product forms a viscous aggregate which may then be injected into the patient at the target site.
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公开(公告)号：US08080521B2
公开(公告)日：2011-12-20
申请号：US12977310
申请日：2010-12-23
CPC分类号： A61K9/0024 , A61K9/0085 , A61K38/177 , A61K38/1774 , A61K38/18 , A61K38/1825 , A61K38/1841 , A61K38/1858 , A61K38/1866 , A61K38/1875 , A61K38/19 , A61K38/30 , A61K47/42 , A61L27/24 , A61L27/54 , A61L27/56 , A61L2300/414
摘要： A carrier matrix may be delivered to a target position within a patient in a minimally invasive manner by first cutting a collagen sponge sheet into a plurality of relatively small pieces. These pieces are sized so that, when wet, they are capable of flowing through a cannula and/or reduced-diameter syringe tip. The pieces are placed into a syringe and wetted, say with a morphogenic solution, and optionally mixed with a bulking material, which is similarly sized to fit through the cannula. The thoroughly mixed and wetted product forms a viscous aggregate which may then be injected into the patient at the target site.
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公开(公告)号：US07825228B2
公开(公告)日：2010-11-02
申请号：US10951236
申请日：2004-09-27
申请人： Gregory A. Hair , Scott D. Boden
发明人： Gregory A. Hair , Scott D. Boden
CPC分类号： C07K14/51 , A61K48/00 , C07K14/47 , C12N2799/021
摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.
摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。
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公开(公告)号：US07671014B2
公开(公告)日：2010-03-02
申请号：US11504363
申请日：2006-08-14
申请人： Neal Beals , Jeffrey L. Scifert , Scott D. Boden
发明人： Neal Beals , Jeffrey L. Scifert , Scott D. Boden
CPC分类号： A61K9/0024 , A61K9/0085 , A61K38/177 , A61K38/1774 , A61K38/18 , A61K38/1825 , A61K38/1841 , A61K38/1858 , A61K38/1866 , A61K38/1875 , A61K38/19 , A61K38/30 , A61K47/42 , A61L27/24 , A61L27/54 , A61L27/56 , A61L2300/414
摘要： A carrier matrix may be delivered to a target position within a patient in a minimally invasive manner by first cutting a collagen sponge sheet into a plurality of relatively small pieces. These pieces are sized so that, when wet, they are capable of flowing through a cannula and/or reduced-diameter syringe tip. The pieces are placed into a syringe and wetted, say with a morphogenic solution, and optionally mixed with a bulking material, which is similarly sized to fit through the cannula. The thoroughly mixed and wetted product forms a viscous aggregate which may then be injected into the patient at the target site.
摘要翻译： 通过首先将胶原海绵片切割成多个相对小的片，可以以微创方式将载体基质递送到患者体内的目标位置。 这些件的尺寸使得在潮湿时它们能够流过套管和/或减小直径的针头。 将片放置在注射器中并用形态发生溶液润湿，并且可选地与填充材料混合，膨胀材料的尺寸类似于穿过套管。 充分混合和润湿的产品形成粘性聚集体，然后可以在目标部位将其注入患者体内。
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58.发明申请Methods and kits using a molecular interaction between a Smurf-1 WW domain and LIM mineralization protein isoforms 有权使用Smurf-1 WW结构域和LIM矿化蛋白质亚型之间的分子相互作用的方法和试剂盒公开(公告)号：US20070190572A1
公开(公告)日：2007-08-16
申请号：US11607348
申请日：2006-12-01
CPC分类号： G01N33/6893 , A61K38/1709 , A61K38/1875 , G01N2333/51 , G01N2500/00 , G01N2800/10 , A61K2300/00
摘要： The instant application provides kits and methods for identifying agents which induce or inhibit the osteogenic effect of LMP or BMP proteins. The kits are directed to methods which measure either an amount of a complex between a Smurf protein or a fragment thereof and an LMP protein or a fragment thereof. Alternatively, the kits are directed to methods of measuring an amount of the ubiquitinated Smad protein or a fragment thereof.
摘要翻译： 本申请提供用于鉴定诱导或抑制LMP或BMP蛋白的成骨作用的试剂的试剂盒和方法。 试剂盒涉及测量Smurf蛋白质或其片段与LMP蛋白质或其片段之间的复合物的量的方法。 或者，试剂盒涉及测量泛素化的Smad蛋白或其片段的量的方法。
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公开(公告)号：US06444803B1
公开(公告)日：2002-09-03
申请号：US09721975
申请日：2000-11-27
申请人： Gregory A. Hair , Scott D. Boden
发明人： Gregory A. Hair , Scott D. Boden
IPC分类号： C07H2104
CPC分类号： C07K14/51 , A61K48/00 , C07K14/47 , C12N2799/021
摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected a osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.
摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过将成骨前体细胞与具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染来融合脊柱的方法，将转染的成骨前体细胞与基质混合并将基质与 脊柱。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。
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