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公开(公告)号：US20060040294A1
公开(公告)日：2006-02-23
申请号：US11183211
申请日：2005-07-15
申请人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Brow , James Dahlberg
发明人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Brow , James Dahlberg
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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公开(公告)号：US20050181435A1
公开(公告)日：2005-08-18
申请号：US11103943
申请日：2005-04-12
申请人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Brow , James Dahlberg
发明人： James Prudent , Jeff Hall , Victor Lyamichev , Mary Brow , James Dahlberg
IPC分类号： G01N33/50 , C12N1/21 , C12N9/12 , C12N9/16 , C12N9/22 , C12N15/09 , C12Q1/68 , G01N33/566 , C07H21/04
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06913881B1
公开(公告)日：2005-07-05
申请号：US09713601
申请日：2000-11-15
申请人： Brian D. Aizenstein , Eric B. Rasmussen , Jeff G. Hall , Poonam Agarwal , David Arco , Myrta W. Atiles , Deborah E. Burris , Monika de Arruda Indig , Scott M. Law , Andrea L. Mast , David J. Marshall , Carolyn W. Miller , Mary C. Oldenberg , James R. Prudent , Jennifer M. Schneiders , Mary Ann Brow , Victor Lyamichev
发明人： Brian D. Aizenstein , Eric B. Rasmussen , Jeff G. Hall , Poonam Agarwal , David Arco , Myrta W. Atiles , Deborah E. Burris , Monika de Arruda Indig , Scott M. Law , Andrea L. Mast , David J. Marshall , Carolyn W. Miller , Mary C. Oldenberg , James R. Prudent , Jennifer M. Schneiders , Mary Ann Brow , Victor Lyamichev
CPC分类号： C12Q1/683 , C12N9/1252 , C12N9/22 , C12Q1/6823 , C12Q1/6844 , C12Q2537/155 , C12Q2525/301 , C12Q2521/301
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在目标序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20070065815A1
公开(公告)日：2007-03-22
申请号：US11515854
申请日：2006-09-05
申请人： Fang Dong , Victor Lyamichev , James Prudent , Lance Fors , Bruce Neri , Mary Brow , Todd Anderson , James Dahlberg
发明人： Fang Dong , Victor Lyamichev , James Prudent , Lance Fors , Bruce Neri , Mary Brow , Todd Anderson , James Dahlberg
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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55.发明申请Compositions and methods for increased dynamic range detection of nucleic acids 审中-公开用于增加核酸动态范围检测的组合物和方法公开(公告)号：US20060246475A1
公开(公告)日：2006-11-02
申请号：US11338244
申请日：2006-01-23
申请人： Patrick Peterson , Hatim Allawi , Victor Lyamichev , Scott Law , Vecheslav Elagin , Jeff Hall
发明人： Patrick Peterson , Hatim Allawi , Victor Lyamichev , Scott Law , Vecheslav Elagin , Jeff Hall
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q1/6827 , C12Q2561/109 , C12Q2537/143 , C12Q2527/143 , C12Q2525/204 , C12Q2525/161 , C12Q2525/301 , C12Q2565/1015
摘要： The present invention provides systems, methods and kits for increasing the dynamic range of detection of a target nucleic acid in a sample. In particular, the present invention provides methods and kits for increasing the dynamic range of detection of a target nucleic acid in a sample through the use of one or more probe oligonucleotides (e.g., analyte-specific probe oligonucleotides).
摘要翻译： 本发明提供用于增加样品中靶核酸的动态检测范围的系统，方法和试剂盒。 特别地，本发明提供了通过使用一种或多种探针寡核苷酸（例如分析物特异性探针寡核苷酸）增加样品中靶核酸的动态检测范围的方法和试剂盒。
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公开(公告)号：US20060183207A1
公开(公告)日：2006-08-17
申请号：US11260845
申请日：2005-10-27
IPC分类号： C12N9/00
CPC分类号： C12N9/1252 , A61K38/00 , C07K2319/02 , C12N9/22 , C12Q1/6823 , C12Q2561/109
摘要： The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明提供了用于切割和修饰核酸的新型切割剂和聚合酶。 裂解剂和聚合酶可用于例如核酸序列的检测和表征以及核酸序列的变化。 在一些实施方案中，使用各种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20060035256A1
公开(公告)日：2006-02-16
申请号：US11198746
申请日：2005-08-05
申请人： James Dahlberg , Mary Ann Brow , Victor Lyamichev
发明人： James Dahlberg , Mary Ann Brow , Victor Lyamichev
CPC分类号： C12N9/1252 , C12Q1/682 , C12Q1/6846 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
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公开(公告)号：US20050186588A1
公开(公告)日：2005-08-25
申请号：US10967711
申请日：2004-10-18
申请人： Victor Lyamichev , Andrew Lukowiak , Nancy Jarvis , David Kurensky , Hatim Allawi , Scott Law , Chad Gerber , Robert Roeven , Jeff Hall
发明人： Victor Lyamichev , Andrew Lukowiak , Nancy Jarvis , David Kurensky , Hatim Allawi , Scott Law , Chad Gerber , Robert Roeven , Jeff Hall
IPC分类号： C12P19/34 , C12Q20060101 , C12Q1/68
CPC分类号： C12Q1/6846 , C12Q2527/119 , C12Q2527/125 , C12Q2561/109
摘要： The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.
摘要翻译： 本发明提供用于开发和优化用于基础研究，临床研究和用于开发临床检测测定的核酸检测测定的方法和程序。 特别地，本发明提供了用于多重扩增反应中使用的寡核苷酸引物的设计方法。 本发明还提供优化多重扩增反应的方法。 本发明还提供了用于组合的靶和信号产生测定的方法。
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公开(公告)号：US20050164177A1
公开(公告)日：2005-07-28
申请号：US09732622
申请日：2000-12-08
申请人： Bruce Neri , Jeff Hall , Victor Lyamichev , Lloyd Smith
发明人： Bruce Neri , Jeff Hall , Victor Lyamichev , Lloyd Smith
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q2525/301 , C12Q2561/109 , C12Q2565/501
摘要： The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a solid support and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征的组合物和方法以及核酸序列的变化。 本发明涉及在固体支持物上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 例如，在一些实施方案中，使用来自任何多种酶的5'核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US20050014163A1
公开(公告)日：2005-01-20
申请号：US10655362
申请日：2003-09-04
申请人： Fang Dong , Victor Lyamichev , James Prudent , Lance Fors , Bruce Neri , Mary Ann Brow , Todd Anderson , James Dahlberg
发明人： Fang Dong , Victor Lyamichev , James Prudent , Lance Fors , Bruce Neri , Mary Ann Brow , Todd Anderson , James Dahlberg
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/701 , C12Q2565/133 , C12Q2521/301
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acids and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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