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    Bacterial cells having a glyoxylate shunt for the manufacture of succinic acid
    62.
    发明授权
    Bacterial cells having a glyoxylate shunt for the manufacture of succinic acid 有权
    具有用于制备琥珀酸的乙醛酸分流的细菌细胞

    公开(公告)号：US08877466B2

    公开(公告)日：2014-11-04

    申请号：US12644059

    申请日：2009-12-22

    申请人： Edzard Scholten ,  Stefan Haefner ,  Hartwig Schröder

    发明人： Edzard Scholten ,  Stefan Haefner ,  Hartwig Schröder

    IPC分类号： C12P7/46 ,  C12N1/20 ,  C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C07H21/04 ,  C12N9/88 ,  C12N9/10 ,  C12N9/02

    CPC分类号： C12N9/88 ,  C12N9/0008 ,  C12N9/1025 ,  C12P7/46

    摘要： The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity. Further, the present invention contemplates a polynucleotide comprising a nucleic acid encoding a polypeptide having isocitrate lyase activity and a nucleic acid encoding a polypeptide having malate synthase activity. Finally, the present invention relates to the use of a bacterial cell of the invention for the manufacture of succinic acid.

    摘要翻译： 本发明涉及生产琥珀酸的细菌。 具体地，本发明涉及巴斯德氏菌属细菌细胞，其包含异柠檬酸裂解酶活性的异源多肽和具有苹果酸合酶活性的异源多肽。 此外，本发明涉及包含编码具有异柠檬酸裂解酶活性的多肽的核酸和编码具有苹果酸合酶活性的多肽的核酸的多核苷酸。 最后，本发明涉及本发明的细菌细胞用于制备琥珀酸的用途。

    BACTERIAL CELLS HAVING A GLYOXYLATE SHUNT FOR THE MANUFACTURE OF SUCCINIC ACID
    63.
    发明申请
    BACTERIAL CELLS HAVING A GLYOXYLATE SHUNT FOR THE MANUFACTURE OF SUCCINIC ACID 有权
    具有用于制备农药酸的谷氨酸盐分离的细菌细胞

    公开(公告)号：US20100159543A1

    公开(公告)日：2010-06-24

    申请号：US12644059

    申请日：2009-12-22

    申请人： Edzard Scholten ,  Stefan Haefner ,  Hartwig Schröder

    发明人： Edzard Scholten ,  Stefan Haefner ,  Hartwig Schröder

    IPC分类号： C12P7/46 ,  C12N1/20 ,  C07H21/00

    CPC分类号： C12N9/88 ,  C12N9/0008 ,  C12N9/1025 ,  C12P7/46

    摘要： The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity. Further, the present invention contemplates a polynucleotide comprising a nucleic acid encoding a polypeptide having isocitrate lyase activity and a nucleic acid encoding a polypeptide having malate synthase activity. Finally, the present invention relates to the use of a bacterial cell of the invention for the manufacture of succinic acid.

    摘要翻译： 本发明涉及生产琥珀酸的细菌。 具体地，本发明涉及巴斯德氏菌属细菌细胞，其包含异柠檬酸裂解酶活性的异源多肽和具有苹果酸合酶活性的异源多肽。 此外，本发明涉及包含编码具有异柠檬酸裂解酶活性的多肽的核酸和编码具有苹果酸合酶活性的多肽的核酸的多核苷酸。 最后，本发明涉及本发明的细菌细胞用于制备琥珀酸的用途。

    Method for producing optically active alcohols from alkanones using a dehydrogenase of azoarcus
    66.
    发明授权
    Method for producing optically active alcohols from alkanones using a dehydrogenase of azoarcus 有权
    使用azoarcus脱氢酶从链烷醇生产光学活性醇的方法

    公开(公告)号：US07785847B2

    公开(公告)日：2010-08-31

    申请号：US11579292

    申请日：2005-05-04

    申请人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Haur ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Haur ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P7/22 ,  C12P7/04 ,  C12P17/00 ,  C12N9/04 ,  C12N9/14 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C07D333/22 ,  C07D333/16 ,  C12P17/00

    摘要： The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

    摘要翻译： 本发明涉及一种制备式（I）的光学活性链烷醇的方法，其中n为0-5的整数; Cyc表示任选取代的单核或多核，饱和或不饱和的碳环或杂环，并且R 1表示卤素，SH，OH，NO 2，NR 2 R 3或NR 2 R 3 R 4 + X - ，其中R 2，R 3和R 4独立地表示H或低级烷基 或低级烷氧基，X-表示抗衡离子。 根据本发明，在含有式（II）的烷酮的培养基中温育选自脱氢酶，醛还原酶和羰基还原酶的酶的酶（E），其中n，R 1和R 1如上定义，在存在下 的还原当量。 将式（II）化合物酶还原成式（I）化合物，并且通过使用酶（E）使牺牲醇与相应的牺牲酮反应，并且至少部分除去牺牲酮来再生反应期间消耗的还原当量 从反应介质中分离出如此制备的产物（I）。

    Bacterial strain and process for the fermentative production of organic acids
    68.
    发明授权
    Bacterial strain and process for the fermentative production of organic acids 有权
    用于发酵生产有机酸的细菌菌株和方法

    公开(公告)号：US08574875B2

    公开(公告)日：2013-11-05

    申请号：US12673714

    申请日：2008-08-14

    申请人： Edzard Scholten ,  Dirk Dägele ,  Stephan Haefner ,  Hartwig Schröder

    发明人： Edzard Scholten ,  Dirk Dägele ,  Stephan Haefner ,  Hartwig Schröder

    IPC分类号： C12P17/10

    CPC分类号： C12P7/46 ,  C12N1/32 ,  C12R1/01

    摘要： The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.

    摘要翻译： 本发明涉及一种称为DD1的新型细菌菌株，其具有生产有机酸特别是琥珀酸（SA）的能力，其最初是从牛瘤胃中分离出来的，能够利用甘油作为碳源; 以及由此导致的变异菌株保留所述能力; 以及通过利用所述微生物生产有机酸，特别是琥珀酸的方法。

    Enzymatic reduction for producing optically active alcohols
    70.
    发明授权
    Enzymatic reduction for producing optically active alcohols 有权
    用于制备光学活性醇的酶还原

    公开(公告)号：US07888080B2

    公开(公告)日：2011-02-15

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II  in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。