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    REDUCING BYPRODUCTION OF MALONATES IN A FERMENTATION PROCESS
    62.
    发明申请
    REDUCING BYPRODUCTION OF MALONATES IN A FERMENTATION PROCESS 有权
    在发酵过程中减少丙二醛的引入

    公开(公告)号：US20100159558A1

    公开(公告)日：2010-06-24

    申请号：US12637877

    申请日：2009-12-15

    申请人： Quinn Qun Zhu ,  Zhixiong Xue

    发明人： Quinn Qun Zhu ,  Zhixiong Xue

    IPC分类号： C12N9/00 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N1/13

    CPC分类号： C12P7/6427 ,  C12N9/93 ,  C12P7/6472

    摘要： Described are methods of reducing the amount of byproduct organic acids during fermentation of an organism, based on expression of a heterologous malonyl-CoA synthetase. A polyunsaturated fatty acid [“PUFA”]-producing strain of the oleaginous yeast Yarrowia lipolytica was engineered to express a heterologous malonyl-CoA synthetase gene. The expression did not effect the production of PUFAs, but did result in a reduced amount of malonates when compared to the amount of malonates produced in the parental strain not expressing malonyl-CoA synthetase.

    摘要翻译： 描述了基于异源丙二酰辅酶A合成酶的表达减少生物体发酵期间副产物有机酸的量的方法。 将多油不饱和脂肪酸[“PUFA”]生产菌株的油质酵母解脂耶氏酵母解脂工程化以表达异源丙二酰辅酶A合成酶基因。 该表达不影响PUFA的产生，但与不表达丙二酰辅酶A合成酶的亲本菌株中产生的丙二酸的量相比，确实导致了丙二酸的量减少。

    DELTA-4 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS
    63.
    发明申请
    DELTA-4 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS 有权
    DELTA-4去饱和及其在制备多糖脂肪酸中的用途

    公开(公告)号：US20090253188A1

    公开(公告)日：2009-10-08

    申请号：US12408860

    申请日：2009-03-23

    申请人： Quinn Qun Zhu ,  Zhixiong Xue

    发明人： Quinn Qun Zhu ,  Zhixiong Xue

    IPC分类号： C12P7/64 ,  C12N9/02 ,  C12N5/10 ,  C12N1/19 ,  C07H21/04

    CPC分类号： C12N9/0083

    摘要： Described here are Δ4 desaturases that convert all-cis-7,10,13,16,19-docosapentaenoic acid [“DPA”; 22:5 ω-3] to docosahexaenoic acid [“DHA”; 22:6 ω-3], with secondary activity in converting docosatetraenoic acid [“DTA”; 22:4 ω-6] to all-cis-4,7,10,13,16-docosapentaenoic acid [“DPAn-6”; 22:5 ω-6]. Also, described here are isolated nuclei acid fragments and recombinant constructs comprising such fragments encoding Δ4 desaturases as well as methods of making long chain polyunsaturated fatty acids [“PUFAs”] using this Δ4 desaturase in oleaginous yeast.

    摘要翻译： 这里描述的是转化全顺式-7,10,13,16,19-二十二碳五烯酸[“DPA”; 22：5ω-3]至二十二碳六烯酸[“DHA”; 22：6ω-3]，其二次活性转化为二十二碳四烯酸[“DTA”; 22：4ω-6]至全顺式-4,7,10,13,16-二十二碳五烯酸[“DPAn-6”; 22：5ω-6]。 此外，这里描述的是分离的核酸片段和包含编码Δ4去饱和酶的片段的重组构建体以及使用该Delta4去饱和酶在含油酵母中制备长链多不饱和脂肪酸[“PUFA”]的方法。

    Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
    64.
    发明授权
    Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids 失效
    衍生自疫霉的合成Delta17去饱和酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US07465793B2

    公开(公告)日：2008-12-16

    申请号：US11787772

    申请日：2007-04-18

    申请人： Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P21/06 ,  C12P7/64 ,  C12N15/70 ,  C12N1/15

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12N9/0087 ,  C12N15/8247 ,  C12P7/6472

    摘要： The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in plants and oleaginous yeast is disclosed.

    摘要翻译： 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的这种片段的重组构建物以及使用植物中的这些Delta17去饱和酶和油性酵母制备长链多不饱和脂肪酸（PUFA）的方法。

    INCREASED OIL CONTENT BY INCREASING YAP1 TRANSCRIPTION FACTOR ACTIVITY IN OLEAGINOUS YEASTS
    65.
    发明申请
    INCREASED OIL CONTENT BY INCREASING YAP1 TRANSCRIPTION FACTOR ACTIVITY IN OLEAGINOUS YEASTS 有权
    通过增加YAP1在油脂中的转录因子活性增加油含量

    公开(公告)号：US20120329160A1

    公开(公告)日：2012-12-27

    申请号：US13339461

    申请日：2011-12-29

    申请人： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    发明人： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC分类号： C12N1/19 ,  C12N15/81

    CPC分类号： C07K14/39 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6463

    摘要： Transgenic oleaginous yeast having increased oil content comprising increased Yap1 transcription factor activity, wherein the increased oil content is compared to the oil content of a non-transgenic oleaginous yeast, are described herein. The increased Yap1 transcription factor activity results from overexpressing a Yap1 transcription factor, by increasing the interaction between the transcription factor and a protein that is capable of activating the transcription factor, or by a combination thereof. Methods of using these yeast strains are also described.

    摘要翻译： 本文描述了具有增加的油含量的转基因含油酵母，其包含增加的Yap1转录因子活性，其中增加的油含量与非转基因油质酵母的油含量进行比较。 通过增加转录因子与能够激活转录因子的蛋白质之间的相互作用，或通过其组合，Yap1转录因子活性的增加来自过表达Yap1转录因子。 还描述了使用这些酵母菌株的方法。

    Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
    67.
    发明授权
    Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效
    高山被孢霉甘油-3-磷酸O-酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量

    公开(公告)号：US07192762B2

    公开(公告)日：2007-03-20

    申请号：US11254173

    申请日：2005-10-19

    申请人： Daniel Joseph Macool ,  Zhixiong Xue

    发明人： Daniel Joseph Macool ,  Zhixiong Xue

    IPC分类号： C12N1/18 ,  C12N15/70 ,  C12N9/10 ,  C12N1/13 ,  C12Q1/68 ,  C12P21/06 ,  C07H21/04 ,  C12N1/19 ,  C12N1/16

    CPC分类号： C12P7/6472 ,  C12N9/1029 ,  C12P7/6463

    摘要： Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

    摘要翻译： 甘油-3-磷酸O-酰基转移酶（GPAT）参与石油生物合成的第一步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（PUFA）的量方面发挥关键作用。 本申请提供了从编码GPAT的高山被孢霉分离的核酸片段，该GPAT适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的GPAT的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母（Yarrowia lipolytica）中积累具有等于或大于C 20的链长度的长链PUFA。

    DIACYLGLYCEROL ACYLTRANSFERASES FOR ALTERATION OF POLYUNSATURATED FATTY ACIDS AND OIL CONTENT IN OLEAGINOUS ORGANISMS
    70.
    发明申请
    DIACYLGLYCEROL ACYLTRANSFERASES FOR ALTERATION OF POLYUNSATURATED FATTY ACIDS AND OIL CONTENT IN OLEAGINOUS ORGANISMS 审中-公开
    用于改变脂肪酸和脂肪酸含量的二油酸甘油酯

    公开(公告)号：US20080020415A1

    公开(公告)日：2008-01-24

    申请号：US11830023

    申请日：2007-07-30

    申请人： Narendra Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    发明人： Narendra Yadav ,  Zhixiong Xue ,  Hongxiang Zhang

    IPC分类号： C12P7/64 ,  C07K14/00 ,  C12N15/12 ,  C40B30/04 ,  C12Q1/48 ,  C12N9/10

    CPC分类号： C12N9/1029 ,  C12P7/6463 ,  C12P7/6472

    摘要： Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.

    摘要翻译： 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。