公开(公告)号：US06176990B1

公开(公告)日：2001-01-23

申请号：US08973933

申请日：1997-12-16

申请人： Thomas D. Yager ,  Paul Waterhouse ,  Alexandre M. Izmailov ,  Bruno C. Maruzzo ,  John K. Stevens ,  Marina T. Larson

发明人： Thomas D. Yager ,  Paul Waterhouse ,  Alexandre M. Izmailov ,  Bruno C. Maruzzo ,  John K. Stevens ,  Marina T. Larson

IPC分类号： G01N27453

CPC分类号： G01N27/44773 ,  G01N27/44704 ,  G01N27/44791

摘要： A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a distance of generally around 1 cm, and are about 1 to 10 &mgr;m wide and 1 to 10 &mgr;m in depth. The channels are filled with a homogeneous separation matrix which acts as an obstacle to the electrophoretic migration of the charged molecules. Microelectrodes disposed in the channels are used to induce an electric field within the homogeneous separation medium. When a voltage is applied across two or more of the microelectrodes, the charged molecules are induced to move and separate according to the electric field density, the type of solvent film, and the charge, shape and size of the charged molecule. The chip may further comprise detectors, such as light polarization detectors, fluorescence emission detectors, biosensors, electrochemical sensors or other microcomponents which may include sites for enzymatic or chemical manipulation of the moved or separated charged molecules.

摘要翻译： 微电泳芯片包括其中形成一个或多个通道的基底，每个待评估样品的通道一个通道。 通道延长芯片的长度，通常约1厘米的距离，宽度约为1至10微米，深度为1至10毫米。 通道充满均匀的分离基质，其作为电荷分子的电泳迁移的障碍。 设置在通道中的微电极用于在均匀分离介质内诱发电场。 当电压施加到两个或更多个微电极上时，根据电场密度，溶剂膜的类型和带电分子的电荷，形状和尺寸，诱导带电分子移动和分离。 芯片还可以包括诸如光偏振检测器，荧光发射检测器，生物传感器，电化学传感器或其它微元件的检测器，其可以包括用于酶或分离带电分子的酶或化学操作的位点。

公开(公告)号：US5710628A

公开(公告)日：1998-01-20

申请号：US353932

申请日：1994-12-12

申请人： Paul Waterhouse ,  John A. Renfrew ,  John K. Stevens

发明人： Paul Waterhouse ,  John A. Renfrew ,  John K. Stevens

IPC分类号： G01N21/64 ,  G01N27/447 ,  G01N21/00

CPC分类号： G01N27/44721

摘要： Improved detection methods and apparatus which may be used individually or in various combinations enhance the ability of the electrophoresis apparatus to detect fluorophore-labeled materials in short periods of time. One such apparatus comprises a housing adapted to receive an electrophoresis gel holder; an excitation source of electromagnetic radiation having a frequency effective to induce emission of electromagnetic radiation from the fluorophore; a plurality of optical fibers for delivering electromagnetic radiation from the excitation source to a linear array of excitation/detection sites on the gel holder, optical switching means for sequentially directing electromagnetic radiation into one of several pre-defined groups of the optical fibers; detection means such as a photomultiplier tube, or an array of photomultiplier tubes for detecting emission from the fluorophore induced by a radiation from the excitation source; and means for correlating a detected emission with the switching of the excitation electromagnetic radiation such that a given emission may be linked with the excitation/detection site being irradiated.

摘要翻译： 可以单独或以各种组合使用的改进的检测方法和装置增强电泳装置在短时间内检测荧光团标记的材料的能力。 一种这样的设备包括适于接收电泳凝胶保持器的壳体; 电磁辐射的激发源具有有效地诱导来自荧光团的电磁辐射的发射的频率; 用于将来自激发源的电磁辐射传递到凝胶保持器上的激发/检测部位的线性阵列的多个光纤，用于将电磁辐射顺序地引导到几个预定义的光纤组中的一个中的光开关装置; 检测装置，例如光电倍增管或用于检测由来自激发源的辐射引起的荧光团发射的光电倍增管阵列; 以及用于将检测到的发射与激发电磁辐射的切换相关联的装置，使得给定的发射可以与被照射的激发/检测部位相关联。

公开(公告)号：US5627022A

公开(公告)日：1997-05-06

申请号：US332577

申请日：1994-11-01

申请人： John A. Renfrew ,  Paul Waterhouse ,  John K. Stevens

发明人： John A. Renfrew ,  Paul Waterhouse ,  John K. Stevens

IPC分类号： G01N27/447 ,  C12Q1/00 ,  C12M1/40

CPC分类号： G01N27/44743 ,  G01N27/44704 ,  G01N27/44747

摘要： An electrophoresis microgel is formed in a gel holder. The gel holder comprises a top substrate, a bottom substrate and a spacer disposed between the top substrate and the bottom substrate. The spacer establishes a separation of from 25 to 250 microns between the top substrate and the bottom substrate. A gel compartment is formed by partially sealing the top substrate to the bottom substrate, while leaving an opening for the introduction of unpolymerized gel. The gel compartment is then filled with an unpolymerized gel, which is polymerized in the gel compartment. Electrodes may be printed on the substrates, may be contacts to an exposed edge of gel, or may be applied through windows cut into one of the substrates. One type of gel holder makes use of graded beads having a diameter of 25 to 250 microns slurried in an adhesive such as an acrylate adhesive as the spacer. The slurry is printed onto the surface of one or both substrates to form a spacer of the desired shape, and then hardened using heat or light. If desired, the spacer can establish lanes within the gel.

摘要翻译： 在凝胶保持器中形成电泳微凝胶。 凝胶保持器包括顶部基底，底部基底和设置在顶部基底和底部基底之间的间隔物。 间隔物在顶部基底和底部基底之间建立了从25至250微米的间隔。 通过将顶部基底部分地密封到底部基底而形成凝胶室，同时留下用于引入未聚合凝胶的开口。 然后用凝胶隔室填充未聚合的凝胶，其在凝胶隔室中聚合。 电极可以印刷在基底上，可以与凝胶的暴露边缘接触，或者可以通过切割成一个基底的窗口来施加。 一种类型的凝胶保持器使用在诸如丙烯酸酯粘合剂的粘合剂中作为间隔物浆化的直径为25至250微米的分级珠粒。 将浆料印刷到一个或两个基材的表面上以形成所需形状的间隔物，然后使用热或光硬化。 如果需要，间隔物可以在凝胶内建立泳道。

公开(公告)号：US20090146804A1

公开(公告)日：2009-06-11

申请号：US12030734

申请日：2008-02-13

申请人： Jason August ,  James Cassidy ,  Robert Griffin ,  John K. Stevens

发明人： Jason August ,  James Cassidy ,  Robert Griffin ,  John K. Stevens

IPC分类号： G08B1/08 ,  G09G3/36

CPC分类号： G06Q30/02

摘要： A two-tiered networked card for identifying and tracking animate and inanimate objects and personnel includes: some components housed in a thick tier of the card and other components housed in the thin tier of the card. The components are: a radio frequency modem operable at a low radio frequency, a loop antenna, a programmable microprocessor, a memory, and a connector for a battery.

摘要翻译： 用于识别和跟踪动画和无生命物体和人员的两层网络卡包括：一些组件容纳在卡的厚层中，以及容纳在该卡的薄层中的其他组件。 组件是：可在低射频操作的射频调制解调器，环形天线，可编程微处理器，存储器和用于电池的连接器。

公开(公告)号：US08714457B2

公开(公告)日：2014-05-06

申请号：US12028483

申请日：2008-02-08

申请人： Jason August ,  James Cassidy ,  Robert Griffin ,  John K. Stevens

发明人： Jason August ,  James Cassidy ,  Robert Griffin ,  John K. Stevens

IPC分类号： G06K19/00 ,  G06Q10/08 ,  A01K11/00 ,  G06K19/07

CPC分类号： G06Q10/08 ,  A01K11/004 ,  A01K11/008 ,  G06K19/0723 ,  G06Q10/087

摘要： A networked card for identifying and tracking animate and inanimate objects and personnel, the card including: a radio frequency modem operable at a low radio frequency not exceeding one megahertz, the modem including a full duplex transmitter and receiver; a loop antenna operatively coupled with the radio frequency modem, the antenna operable within a range of hundreds of feet; a programmable microprocessor operatively coupled with the radio frequency modem and fully programmable within a network; a memory operatively coupled with the programmable microprocessor, the memory holding identification data; and a battery for providing power to the microprocessor.

摘要翻译： 一种用于识别和跟踪动画和无生命物体和人员的联网卡，所述卡包括：可在不超过一兆赫兹的低射​​频操作的无线电频率调制解调器，所述调制解调器包括全双工发射器和接收器; 可操作地与射频调制解调器耦合的环形天线，天线可在数百英尺的范围内操作; 可操作地与无线电频率调制解调器耦合并且可在网络内完全可编程的可编程微处理器; 与所述可编程微处理器可操作地耦合的存储器，所述存储器保存识别数据; 以及用于向微处理器供电的电池。

公开(公告)号：US20090212117A1

公开(公告)日：2009-08-27

申请号：US12028483

申请日：2008-02-08

申请人： Jason August ,  James Cassidy ,  Robert Griffin ,  John K. Stevens

发明人： Jason August ,  James Cassidy ,  Robert Griffin ,  John K. Stevens

IPC分类号： G06K19/06

CPC分类号： G06Q10/08 ,  A01K11/004 ,  A01K11/008 ,  G06K19/0723 ,  G06Q10/087

摘要： A networked card for identifying and tracking animate and inanimate objects and personnel, the card including: a radio frequency modem operable at a low radio frequency not exceeding one megahertz, the modem including a full duplex transmitter and receiver; a loop antenna operatively coupled with the radio frequency modem, the antenna operable within a range of hundreds of feet; a programmable microprocessor operatively coupled with the radio frequency modem and fully programmable within a network; a memory operatively coupled with the programmable microprocessor, the memory holding identification data; and a battery for providing power to the microprocessor.

摘要翻译： 一种用于识别和跟踪动画和无生命物体和人员的联网卡，所述卡包括：可在不超过一兆赫兹的低射​​频操作的无线电频率调制解调器，所述调制解调器包括全双工发射器和接收器; 可操作地与射频调制解调器耦合的环形天线，天线可在数百英尺的范围内操作; 可编程微处理器，可操作地与射频调制解调器耦合，并在网络内完全可编程; 与所述可编程微处理器可操作地耦合的存储器，所述存储器保存识别数据; 以及用于向微处理器供电的电池。

公开(公告)号：US20100322168A1

公开(公告)日：2010-12-23

申请号：US12705115

申请日：2010-02-12

申请人： John K. Stevens ,  David Cramer ,  Tom Packert

发明人： John K. Stevens ,  David Cramer ,  Tom Packert

IPC分类号： H04W4/00

CPC分类号： G06Q10/087

摘要： The system employs full transceivers, each having peer-to-peer, client/server, and IP networking capabilities, and covering open-area ranges of up to 100 feet. The system uses Low Frequency for data communications so it can achieve both low cost (less costly than many RF-ID tags) and long battery life (10-15 years). Additionally, since these tags have batteries, static RAM maybe be added at very low cost, as well as sensors, LED's displays etc. The system also employs a sidewinder that communicates to said tag regularly, said sidewinder keeps the IP address of said tag. The system also employs an embedded VPN. The VPN includes a suite of diagnostic tools that run on a laptop. The diagnostic tools talk direct to the sidewinder, the tools read and program said tag providing the tools have correct IP address of the tag. The system also includes a visibility data server that communicates to the sidewinder by the VPN. The data server includes a virtual tag data base, said virtual tag data base updated by the sidewinder. The web enabled reports and control are managed by two “plugs”. The SQL plug supports data requests, and the Air Traffic Control (ATC) plug provides control (LED's, read rates). The system also employs a user web based ERP to create a real-time visibility reports and a user event export tools to create reports that fit a specific event.

摘要翻译： 该系统采用完整的收发器，每个收发器具有点对点，客户端/服务器和IP网络功能，并覆盖高达100英尺的开放范围。 该系统使用低频进行数据通信，因此可以实现低成本（比许多RF-ID标签更便宜）和更长的电池寿命（10-15年）。 此外，由于这些标签具有电池，因此可以以非常低的成本添加静态RAM，以及传感器，LED显示器等。该系统还采用定期与所述标签通信的侧卷器，所述侧卷纸保持所述标签的IP地址。 该系统还采用嵌入式VPN。 VPN包括一套在膝上型计算机上运行的诊断工具。 诊断工具直接与副卷轴通信，读取和编程所述标签的工具提供工具具有正确的标签IP地址。 该系统还包括一个可视数据服务器，它通过VPN与主持人进行通信。 数据服务器包括虚拟标签数据库，所述虚拟标签数据库由所述侧挂方更新。 网络启用报告和控制由两个“插头”管理。 SQL插件支持数据请求，空中交通管制（ATC）插头提供控制（LED，读取速率）。 该系统还使用基于用户网络的ERP来创建实时可见性报告和用户事件导出工具来创建适合特定事件的报告。

公开(公告)号：US6063567A

公开(公告)日：2000-05-16

申请号：US779916

申请日：1997-01-07

申请人： Brenda L. Gallie ,  James M. Dunn ,  John K. Stevens ,  May Hui

发明人： Brenda L. Gallie ,  James M. Dunn ,  John K. Stevens ,  May Hui

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6886 ,  C12Q2600/16

摘要： Reliable and cost effective testing for mutations in the RB1 gene can be accomplished by (a) quantitatively amplifying exons of the sample RB1 gene using primers complementary to intron regions flanking each exon; and (b) determining the lengths and/or quantities of the amplification products for each exon and comparing that length or quantity to the length or quantity of amplification products obtained when a wild-type RB1 gene is amplified using the same primers. Differences in length between an amplified sample exon and the corresponding amplified wild-type exon reflect the occurrence on an insertion or deletion mutation in the sample RB1 gene. Differences in quantity reflect the complete absence of an exon, or heterozygosity for a mutant exon. Next, the nucleic acid sequence of each exon found to contain an insertion or deletion mutation is determined, or of all exons in the event no insertion or deletion mutations are identified. Preferably, the amplification of the exons is multiplexed so that more than one exon is amplified in a single vessel using sets of primers which provide gene fragments of distinctive lengths when used to amplify a normal RB1 gene.

摘要翻译： 可以通过（a）使用与每个外显子侧翼的内含子区域互补的引物定量扩增样品RB1基因的外显子来实现对RB1基因突变的可靠和成本有效的测试; 和（b）确定每个外显子的扩增产物的长度和/或数量，并将该长度或数量与使用相同引物扩增野生型RB1基因时获得的扩增产物的长度或数量进行比较。 扩增样本外显子与相应的扩增野生型外显子之间的长度差异反映样品RB1基因插入或缺失突变的发生。 量的差异反映了突变外显子完全不存在外显子或杂合性。 接下来，确定发现含有插入或缺失突变的每个外显子的核酸序列，或者在没有鉴定插入或缺失突变的情况下所有外显子的核酸序列。 优选地，外显子的扩增被多重化，使得在用于扩增正常RB1基因时提供具有特异长度的基因片段的引物组在单个容器中扩增多于一个外显子。

公开(公告)号：US4879756A

公开(公告)日：1989-11-07

申请号：US141242

申请日：1988-01-06

申请人： John K. Stevens ,  Paul I. Waterhouse

发明人： John K. Stevens ,  Paul I. Waterhouse

IPC分类号： G06K17/00 ,  H04L5/14 ,  H04L27/18

CPC分类号： G06K17/0022 ,  H04L27/18 ,  H04L5/1492 ,  G09G2380/04

摘要： The invention comprises a low power broadcast system that is applicable especially to the so-called "electronic shelf" for retail stores, wherein the shelf edge carries price displaying modules that can be addressed and controlled from a central computer operated station. The system also permits the modules to broadcast back to the central station to confirm safe receipt of data and to give information as to stock levels, etc. A broadcast system avoids the need for wiring so that location changes are facilitated. To overcome the extremely noisy environment and to conserve power consumption, and hence battery life, the system employs a low frequency (132 kHz) reference carrier transmitted by the base station in discrete segmented packages, each of which frames a base data word transmitted by the base station and a corresponding module data word transmitted by the module a fixed period after the end of the base word; the base receiver then has precise time information for receipt of the module word and can "look" for it among the noise. The carrier received by the module is divided and the lower frequency used to demodulate the information-carrying transmission from the base station of the same frequency, avoiding the need for a phase locked loop detector; this lower frequency is also used for the module transmission. The module employs an air-cored loop antenna coil for the lower frequency and a ferrite-cored loop antenna for the higher reference frequency, while the store antenna is segmented for selection of the group of modules to be addressed; the antenna contacts the metal shelving to provide electromagnetic coupling thereto. Each module contains a microprocessor which controls the operation. Each module has "concealed" buttons which can be enabled and used to insert data to be transmitted therefrom. A charging circuit can be used as the power source employing the received RF carrier energy.

摘要翻译： 本发明包括一种低功率广播系统，其特别适用于零售商店的所谓“电子货架”，其中货架边缘携带可从中央计算机操作的站址寻址和控制的价格显示模块。 该系统还允许模块广播回到中央站，以确认数据的安全接收并提供关于库存等级的信息等。广播系统避免了对布线的需要，从而便于位置改变。 为了克服极其嘈杂的环境并节省电力消耗，并因此节省电池寿命，该系统采用由基站在离散分段封装中传输的低频（132kHz）参考载波，其中每个包含由 基站和相应的模块数据字在模块结束后固定的时间段发送; 基地接收机然后具有用于接收模块字的精确时间信息，并且可以在噪声中“寻找”它。 由模块接收的载波被划分，较低的频率用于解调来自相同频率的基站的信息传输传输，避免了对锁相环检测器的需要; 该较低频率也用于模块传输。 该模块采用较低频率的空心环形天线线圈和用于更高参考频率的铁氧体磁芯环形天线，而存储天线被分段以选择要寻址的模块组; 天线接触金属搁架以提供电磁耦合。 每个模块都包含一个控制操作的微处理器。 每个模块都具有“隐藏”按钮，可以启用并将其用于插入要传输的数据。 充电电路可以用作采用所接收的RF载波能量的电源。

公开(公告)号：US08115628B2

公开(公告)日：2012-02-14

申请号：US12705115

申请日：2010-02-12

申请人： John K. Stevens ,  David Cramer ,  Tom Packert

发明人： John K. Stevens ,  David Cramer ,  Tom Packert

IPC分类号： G08B13/14 ,  G06Q10/00 ,  G06F7/00

CPC分类号： G06Q10/087

摘要： The system employs full transceivers, each having peer-to-peer, client/server, and IP networking capabilities, and covering open-area ranges of up to 100 feet. The system uses Low Frequency for data communications so it can achieve both low cost (less costly than many RF-ID tags) and long battery life (10-15 years). Additionally, since these tags have batteries, static RAM maybe be added at very low cost, as well as sensors, LED's displays etc. The system also employs a sidewinder that communicates to said tag regularly, said sidewinder keeps the IP address of said tag. The system also employs an embedded VPN. The VPN includes a suite of diagnostic tools that run on a laptop. The system also includes a visibility data server that communicates to the sidewinder by the VPN. The data server includes a virtual tag data base, said virtual tag data base updated by the sidewinder.

摘要翻译： 该系统采用完整的收发器，每个收发器具有点对点，客户端/服务器和IP网络功能，并覆盖高达100英尺的开放范围。 该系统使用低频进行数据通信，因此可以实现低成本（比许多RF-ID标签更便宜）和更长的电池寿命（10-15年）。 此外，由于这些标签具有电池，因此可以以非常低的成本添加静态RAM，以及传感器，LED显示器等。该系统还采用定期与所述标签通信的侧卷器，所述侧卷纸保持所述标签的IP地址。 该系统还采用嵌入式VPN。 VPN包括一套在膝上型计算机上运行的诊断工具。 该系统还包括一个可视数据服务器，它通过VPN与主持人进行通信。 数据服务器包括虚拟标签数据库，所述虚拟标签数据库由所述侧挂方更新。