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147 条记录 (耗时 0.087 秒)

    Nucleotide sugar purification using membranes
    65.
    发明授权
    Nucleotide sugar purification using membranes 有权
    使用膜的核苷酸糖纯化

    公开(公告)号：US08841439B2

    公开(公告)日：2014-09-23

    申请号：US13215439

    申请日：2011-08-23

    申请人： Michael Felo ,  Shawn DeFrees

    发明人： Michael Felo ,  Shawn DeFrees

    IPC分类号： C07H19/10 ,  C07H1/06 ,  C12P19/30 ,  C07H19/06

    CPC分类号： C12P19/305 ,  C07H19/06 ,  Y02P20/582

    摘要： The invention provides methods of removing contaminants from a mixture of a desired product and contaminants by pH adjustments and molecular weight cut-offs. The contaminants include phosphate groups, magnesium sulfate, sodium pyruvate and tetrasodium pyrophosphate groups. The desired product includes nucleotide sugars, glycolipids, LnNT, sialyl lactose, and salts.

    摘要翻译： 本发明提供了通过pH调节和分子量截留从所需产物和污染物的混合物中除去污染物的方法。 污染物包括磷酸盐基团，硫酸镁，丙酮酸钠和焦磷酸四钠基团。 所需产物包括核苷酸糖，糖脂，LnNT，唾液酸乳糖和盐。

    Branched water-soluble polymers and their conjugates
    66.
    发明授权
    Branched water-soluble polymers and their conjugates 有权
    支链水溶性聚合物及其共轭物

    公开(公告)号：US08247381B2

    公开(公告)日：2012-08-21

    申请号：US12858247

    申请日：2010-08-17

    申请人： Shawn DeFrees

    发明人： Shawn DeFrees

    IPC分类号： A01N43/04 ,  A61K31/70

    CPC分类号： C07H19/20 ,  A61K38/00 ,  A61K47/60 ,  C07C237/12 ,  C07C271/22 ,  C07C323/59 ,  C07K1/13 ,  C07K5/022 ,  C07K5/0821 ,  C07K9/001

    摘要： The present invention provides branched water-soluble polymers that allow two or more water-soluble polymers to be conjugated to another species. The branched polymers provide access to therapeutic agents that are conjugated at a single site to two or more water-soluble polymers. The branched polymers are based upon branch points that are simple branched alkyl structures, reactive side-chain amino acids and small peptides of reactive side-chain amino acids, and saccharides. Also provided is a method for preparing mono-disperse poly(ethylene glycol) of a well-defined and determinable molecular weight, and a method for the rational end-functionalization of poly(ethylene glycol). Conjugates of the branched water-soluble polymers with diverse species, e.g., peptides, lipids, glycolipids and small molecules are also provided.

    摘要翻译： 本发明提供了允许两种或更多种水溶性聚合物与另一种物质结合的支链水溶性聚合物。 支链聚合物提供了在单个位点与两个或多个水溶性聚合物共轭的治疗剂。 支链聚合物基于分支点，其为简单的支链烷基结构，反应性侧链氨基酸和反应性侧链氨基酸的小肽，以及糖。 还提供了制备具有明确且可确定的分子量的单分散聚（乙二醇）的方法，以及聚（乙二醇）的合理末端官能化的方法。 还提供了具有不同种类的支链水溶性聚合物的缀合物，例如肽，脂质，糖脂和小分子。

    REMODELING AND GLYCOPEGYLATION OF FIBROBLAST GROWTH FACTOR (FGF)
    67.
    发明申请
    REMODELING AND GLYCOPEGYLATION OF FIBROBLAST GROWTH FACTOR (FGF) 有权
    纤维母细胞生长因子（FGF）的重组和糖蛋白

    公开(公告)号：US20120172300A1

    公开(公告)日：2012-07-05

    申请号：US13332708

    申请日：2011-12-21

    申请人： Shawn DeFrees

    发明人： Shawn DeFrees

    IPC分类号： A61K38/18 ,  A61P17/02 ,  A61P17/00 ,  A61P9/10 ,  C12P21/00 ,  A61P25/00 ,  A61P27/02 ,  A61P35/00 ,  A61P3/10 ,  C07K14/50 ,  A61P29/00

    CPC分类号： A61K38/1825 ,  A61K38/00 ,  A61K47/549 ,  A61K47/60 ,  C07K14/50 ,  C12P21/005

    摘要： The present invention relates to mutants of Fibroblast Growth Factor (FGF), particularly FGF-20 and FGF-21, which contain newly introduced N-linked or O-linked glycosylation site(s). The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

    摘要翻译： 本发明涉及含有新引入的N-连接或O-连接的糖基化位点的成纤维细胞生长因子（FGF），特别是FGF-20和FGF-21的突变体。 还公开了突变体的多核苷酸编码序列，包含编码序列的表达盒，表达突变体的细胞，以及用于产生突变体的方法。 还公开了包含突变体和使用突变体的方法的药物组合物。

    ENZYMATIC MODIFICATION OF GLYCOPEPTIDES
    68.
    发明申请
    ENZYMATIC MODIFICATION OF GLYCOPEPTIDES 审中-公开
    糖化酶的酶学修饰

    公开(公告)号：US20110318780A1

    公开(公告)日：2011-12-29

    申请号：US13163473

    申请日：2011-06-17

    申请人： Shawn DeFrees

    发明人： Shawn DeFrees

    IPC分类号： C12P21/00 ,  C12P21/06

    CPC分类号： C12P21/005

    摘要： The present invention provides glycoconjugates that are formed through the enzymatically-mediated coupling of a glycosyl moiety, e.g., on a peptide or lipid, and a modifying group that includes an acyl group. The conjugates include the modifying group tethered to the glycosyl moiety through a linking moiety that includes an acyl residue. Also provided are methods for preparing the conjugates of the invention

    摘要翻译： 本发明提供了通过酶促介导的糖基部分（例如肽或脂质）和包含酰基的修饰基团的酶促介导的偶联形成的糖缀合物。 缀合物包括通过包含酰基残基的连接部分连接到糖基部分的修饰基团。 还提供了制备本发明共轭物的方法

    Compositions and methods for the preparation of human growth hormone glycosylation mutants
    70.
    发明授权
    Compositions and methods for the preparation of human growth hormone glycosylation mutants 有权
    用于制备人生长激素糖基化突变体的组合物和方法

    公开(公告)号：US07932364B2

    公开(公告)日：2011-04-26

    申请号：US10556094

    申请日：2004-05-07

    申请人： Shawn DeFrees ,  Henrik Clausen

    发明人： Shawn DeFrees ,  Henrik Clausen

    IPC分类号： C07K1/00 ,  C07K14/00

    CPC分类号： C07K14/61 ,  A61K38/00

    摘要： The present invention relates to mutants of human growth hormone, which contain newly introduced N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

    摘要翻译： 本发明涉及包含新引入的N-连接或O-连接的糖基化位点的人生长激素的突变体，使得这些重组产生的多肽具有与天然存在的人生长激素的糖基化模式明显不同的糖基化模式。 还公开了突变体的多核苷酸编码序列，包含编码序列的表达盒，表达突变体的细胞，以及用于产生突变体的方法。 还公开了包含突变体和使用突变体的方法的药物组合物。