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    O-linked glycosylation of peptides
    1.
    发明申请
    O-linked glycosylation of peptides 有权
    肽的O-连接糖基化

    公开(公告)号：US20090169509A1

    公开(公告)日：2009-07-02

    申请号：US10585385

    申请日：2005-01-10

    申请人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    发明人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    IPC分类号： A61K38/21 ,  C07K7/00 ,  C07K14/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12P21/02 ,  A61K38/16

    CPC分类号： A61K38/193 ,  C07K1/1077 ,  C07K9/00 ,  C07K9/001 ,  C07K14/535

    摘要： The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

    摘要翻译： 本发明提供了包含在野生型肽中不存在的O-连接的糖基化位点的多肽。 本发明的多肽包括糖缀合物，其中诸如水溶性聚合物的物质，生物分子的治疗剂通过完整的O连接的糖基残基与多肽共价连接。 还提供了制备本发明的肽和方法的方法，含有肽的药物组合物和通过施用足够达到所需反应的量的本发明的肽来治疗，改善或预防哺乳动物患病的方法。

    O-linked glycosylation of peptides
    2.
    发明授权
    O-linked glycosylation of peptides 有权
    肽的O-连接糖基化

    公开(公告)号：US07338933B2

    公开(公告)日：2008-03-04

    申请号：US11033365

    申请日：2005-01-10

    申请人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    发明人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    IPC分类号： A61K38/16 ,  A61K38/00

    CPC分类号： A61K38/193 ,  C07K1/1077 ,  C07K9/00 ,  C07K9/001 ,  C07K14/535

    摘要： The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

    摘要翻译： 本发明提供了包含在野生型肽中不存在的O-连接的糖基化位点的多肽。 本发明的多肽包括糖缀合物，其中诸如水溶性聚合物的物质，生物分子的治疗剂通过完整O-连接的糖基残基与多肽共价连接。 还提供了制备本发明的肽和方法的方法，含有肽的药物组合物和通过施用足够达到所需反应的量的本发明的肽来治疗，改善或预防哺乳动物患病的方法。

    Compositions and methods for the preparation of human growth hormone glycosylation mutants
    3.
    发明授权
    Compositions and methods for the preparation of human growth hormone glycosylation mutants 有权
    用于制备人生长激素糖基化突变体的组合物和方法

    公开(公告)号：US07932364B2

    公开(公告)日：2011-04-26

    申请号：US10556094

    申请日：2004-05-07

    申请人： Shawn DeFrees ,  Henrik Clausen

    发明人： Shawn DeFrees ,  Henrik Clausen

    IPC分类号： C07K1/00 ,  C07K14/00

    CPC分类号： C07K14/61 ,  A61K38/00

    摘要： The present invention relates to mutants of human growth hormone, which contain newly introduced N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

    摘要翻译： 本发明涉及包含新引入的N-连接或O-连接的糖基化位点的人生长激素的突变体，使得这些重组产生的多肽具有与天然存在的人生长激素的糖基化模式明显不同的糖基化模式。 还公开了突变体的多核苷酸编码序列，包含编码序列的表达盒，表达突变体的细胞，以及用于产生突变体的方法。 还公开了包含突变体和使用突变体的方法的药物组合物。

    Glycopegylated interferon α
    5.
    发明授权
    Glycopegylated interferon α 有权
    糖基化干扰素α

    公开(公告)号：US08268967B2

    公开(公告)日：2012-09-18

    申请号：US11659942

    申请日：2005-09-12

    申请人： Shawn DeFrees ,  David A. Zopf ,  Henrik Clausen ,  Ruye Xing

    发明人： Shawn DeFrees ,  David A. Zopf ,  Henrik Clausen ,  Ruye Xing

    IPC分类号： C07K14/56 ,  A61K38/21

    CPC分类号： A61K38/212 ,  A61K47/60 ,  Y02A50/463 ,  Y02A50/473

    摘要： The present invention provides IFN-α conjugates including IFN-α peptides and modifying groups such as PEG moieties. The IFN-α peptide and modifying group are linked via an intact glycosyl linking group interposed between and covalently attached to the IFN-α peptide and the modifying group. The IFN-α conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar onto an amino acid or a glycosyl residue on the IFN-α peptide. Also provided are methods for preparing the IFN-α conjugates, methods for treating various disease conditions with the IFN-α conjugates, and pharmaceutical formulations including the IFN-α conjugates.

    摘要翻译： 本发明提供IFN-α缀合物，包括IFN-α肽和修饰基团例如PEG部分。 IFN-α肽和修饰基团通过介于IFN-α肽和修饰基团之间并共价连接的完整糖基连接基团连接。 通过糖基转移酶的作用由糖基化肽形成IFN-α缀合物。 糖基转移酶将修饰的糖连接到IFN-α肽上的氨基酸或糖基残基上。 还提供了制备IFN-α缀合物的方法，用IFN-α缀合物治疗各种疾病状况的方法，以及包括IFN-α缀合物的药物制剂。

    O-LINKED GLYCOSYLATION OF PEPTIDES
    6.
    发明申请
    O-LINKED GLYCOSYLATION OF PEPTIDES 审中-公开
    肽连接的糖蛋白

    公开(公告)号：US20080242846A1

    公开(公告)日：2008-10-02

    申请号：US11843588

    申请日：2007-08-22

    申请人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    发明人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    IPC分类号： C07K14/00 ,  C07K1/00

    CPC分类号： A61K38/193 ,  C07K1/1077 ,  C07K9/00 ,  C07K9/001 ,  C07K14/535

    摘要： The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

    摘要翻译： 本发明提供了包含在野生型肽中不存在的O-连接的糖基化位点的多肽。 本发明的多肽包括糖缀合物，其中诸如水溶性聚合物的物质，生物分子的治疗剂通过完整O-连接的糖基残基与多肽共价连接。 还提供了制备本发明的肽和方法的方法，含有肽的药物组合物和通过施用足够达到所需反应的量的本发明的肽来治疗，改善或预防哺乳动物患病的方法。

    Compositions and Methods for the Preparation of Human Growth Hormone Glycosylation Mutants
    7.
    发明申请
    Compositions and Methods for the Preparation of Human Growth Hormone Glycosylation Mutants 有权
    用于制备人生长激素糖基化突变体的组合物和方法

    公开(公告)号：US20080102083A1

    公开(公告)日：2008-05-01

    申请号：US10556094

    申请日：2004-05-07

    申请人： Shawn DeFrees ,  Henrik Clausen

    发明人： Shawn DeFrees ,  Henrik Clausen

    IPC分类号： A61K39/00 ,  A61P43/00 ,  C07H21/04 ,  C07K16/00 ,  C12N5/00 ,  C12P21/04

    CPC分类号： C07K14/61 ,  A61K38/00

    摘要： The present invention relates to mutants of human growth hormone, which contain newly introduced N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.

    摘要翻译： 本发明涉及包含新引入的N-连接或O-连接的糖基化位点的人生长激素的突变体，使得这些重组产生的多肽具有与天然存在的人生长激素明显不同的糖基化模式。 还公开了突变体的多核苷酸编码序列，包含编码序列的表达盒，表达突变体的细胞，以及用于产生突变体的方法。 还公开了包含突变体和使用突变体的方法的药物组合物。

    O-linked glycosylation of peptides
    8.
    发明申请
    O-linked glycosylation of peptides 有权
    肽的O-连接糖基化

    公开(公告)号：US20050250678A1

    公开(公告)日：2005-11-10

    申请号：US11033365

    申请日：2005-01-10

    申请人： Shawn DeFrees ,  David Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    发明人： Shawn DeFrees ,  David Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    IPC分类号： A61K38/14 ,  A61K38/19 ,  C07K1/107 ,  C07K9/00 ,  C07K14/535

    CPC分类号： A61K38/193 ,  C07K1/1077 ,  C07K9/00 ,  C07K9/001 ,  C07K14/535

    摘要： The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

    摘要翻译： 本发明提供了包含在野生型肽中不存在的O-连接的糖基化位点的多肽。 本发明的多肽包括糖缀合物，其中诸如水溶性聚合物的物质，生物分子的治疗剂通过完整O-连接的糖基残基与多肽共价连接。 还提供了制备本发明的肽和方法的方法，含有肽的药物组合物和通过施用足够达到所需反应的量的本发明的肽来治疗，改善或预防哺乳动物患病的方法。

    PURIFICATION OF PEPTIDE CONJUGATES BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY
    10.
    发明申请
    PURIFICATION OF PEPTIDE CONJUGATES BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY 审中-公开
    通过疏水相互作用色谱法纯化肽结合

    公开(公告)号：US20120016105A1

    公开(公告)日：2012-01-19

    申请号：US13186726

    申请日：2011-07-20

    申请人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    发明人： Shawn DeFrees ,  David A. Zopf ,  Zhi-Guang Wang ,  Henrik Clausen

    IPC分类号： C07K1/20 ,  C07K14/755

    CPC分类号： A61K38/193 ,  C07K1/1077 ,  C07K9/00 ,  C07K9/001 ,  C07K14/535

    摘要： The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making a glycoconjugate, methods of isolating a glycoconjugate from a reaction mixture, pharmaceutical compositions containing a glycoconjugate, and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a glycoconjugate sufficient to achieve the desired response.

    摘要翻译： 本发明提供了包含在野生型肽中不存在的O-连接的糖基化位点的多肽。 本发明的多肽包括糖缀合物，其中诸如水溶性聚合物的物质，生物分子的治疗剂通过完整O-连接的糖基残基与多肽共价连接。 还提供了制备糖缀合物的方法，从反应混合物中分离糖缀合物的方法，含有糖缀合物的药物组合物，以及通过施用足以达到所需反应的量的糖缀合物来治疗，改善或预防哺乳动物患病的方法。