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    Art (rev) protein of human T-cell leukemia virus
    67.
    发明授权
    Art (rev) protein of human T-cell leukemia virus 失效
    人类T细胞白血病病毒的Art（rev）蛋白

    公开(公告)号：US5321124A

    公开(公告)日：1994-06-14

    申请号：US995948

    申请日：1992-12-18

    申请人： William A. Haseltine ,  Craig A. Rosen ,  Joseph G. Sodroski ,  Wei C. Goh

    发明人： William A. Haseltine ,  Craig A. Rosen ,  Joseph G. Sodroski ,  Wei C. Goh

    IPC分类号： C12N15/00 ,  A61K39/12 ,  C07K1/22 ,  C07K14/00 ,  C07K14/155 ,  C07K14/16 ,  C12N5/00 ,  C12N15/85 ,  C12P21/02 ,  C12R1/91 ,  C07K13/00

    CPC分类号： C07K14/005 ,  C12N15/85 ,  C12N2740/14022 ,  C12N2740/16322 ,  Y10S930/221

    摘要： A gene and gene product that regulates the expression of the capsidal envelope genes of HTLV-III/LAV and that can be used to regulate the expression of heterologous (non-viral) genes as well is disclosed. This art gene consists of two exons and can be used in creating nucleotide segments, vectors and cell lines. A new method for screening for compounds that inhibit the replication of HTLV-III is also described and comprises:(1) transfecting a T-cell line with the HTLV-III art and env genes;(2) thereafter, adding a preselected compound to the transformed cell line in increasing concentrations; and(3) determining whether the compound effects the art function without being toxic to the cell.An additional parameter to use in diagnosis of AIDS disease is also described. The use of the art gene and gene product in AIDS therapy is also disclosed.

    摘要翻译： 公开了一种调节HTLV-III / LAV的包膜基因表达并可用于调节异源（非病毒）基因表达的基因和基因产物。 该艺术基因由两个外显子组成，可用于产生核苷酸片段，载体和细胞系。 还描述了用于筛选抑制HTLV-III复制的化合物的新方法，其包括：（1）用HTLV-III技术和env基因转染T细胞系; （2）之后，以增加的浓度向转化细胞系中加入预选化合物; 和（3）确定化合物是否影响艺术功能而不对细胞有毒性。 还描述了用于诊断AIDS疾病的另外的参数。 还公开了在艾滋病治疗中使用艺术基因和基因产物。