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137 条记录 (耗时 0.059 秒)

    Methods for Detection and Quantification of Analytes in Complex Mixtures
    62.
    发明申请
    Methods for Detection and Quantification of Analytes in Complex Mixtures 有权
    复杂混合物中分析物的检测和定量方法

    公开(公告)号：US20140162251A1

    公开(公告)日：2014-06-12

    申请号：US13794299

    申请日：2013-03-11

    申请人： THE INSTITUTE FOR SYSTEMS BIOLOGY

    发明人： Krassen M. Dimitrov ,  Dwayne L. Dunaway

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6888 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6876 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2525/161 ,  C12Q2565/1025 ,  C12Q2563/179 ,  C12Q2525/151 ,  C12Q2525/101

    摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.

    摘要翻译： 本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。 还提供了生产独特标记的核酸探针群体的方法。 还提供了检测核酸分析物的方法。 该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗基因组杂交的条件下接触混合物，所述多个抗Genetigits具有足以独特地与指定剂内的Genesigits杂交的分集，以及（c）唯一检测 混合物中一种或多种分析物之间的杂交复合物，靶标特异性探针和抗Genetigigit。

    Methods for identifying and monitoring drug side effects
    64.
    发明授权
    Methods for identifying and monitoring drug side effects 有权

    公开(公告)号：US08603752B2

    公开(公告)日：2013-12-10

    申请号：US13023366

    申请日：2011-02-08

    申请人： Leroy Hood ,  Biaoyang Lin

    发明人： Leroy Hood ,  Biaoyang Lin

    IPC分类号： G01N33/53

    CPC分类号： G01N33/57434 ,  G01N33/6842 ,  G01N33/6893

    摘要： The present invention relates generally to methods for identifying drug side effects by detecting perturbations in organ-specific molecular blood fingerprints. The invention further relates to methods for identifying drug-specific organ-specific molecular blood fingerprints. As such, the present invention provides compositions comprising organ-specific proteins, detection reagents for detecting such proteins, and panels and arrays for determining organ-specific molecular blood fingerprints.

    Methods for detection and quantification of analytes in complex mixtures
    65.
    发明授权
    Methods for detection and quantification of analytes in complex mixtures 有权
    复杂混合物中分析物的检测和定量方法

    公开(公告)号：US08492094B2

    公开(公告)日：2013-07-23

    申请号：US13027493

    申请日：2011-02-15

    申请人： Krassen Dimitrov ,  Dwayne L. Dunaway

    发明人： Krassen Dimitrov ,  Dwayne L. Dunaway

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6888 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6876 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2525/161 ,  C12Q2565/1025 ,  C12Q2563/179 ,  C12Q2525/151 ,  C12Q2525/101

    摘要： The invention provides a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of antigenedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.

    摘要翻译： 本发明提供了一种检测核酸分析物的方法。 该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗原多肽杂交的条件下使混合物接触，所述多个抗Genetigits具有足以独特地与指定剂内的genesigits杂交的多样性，以及（c）唯一地检测杂交的 混合物中一种或多种分析物之间的复合物，目标特异性探针和抗Genetigigit。

    METHODS TO DETECT AND QUANTIFY RNA
    67.
    发明申请
    METHODS TO DETECT AND QUANTIFY RNA 有权
    检测和定量RNA的方法

    公开(公告)号：US20120283106A1

    公开(公告)日：2012-11-08

    申请号：US13397572

    申请日：2012-02-15

    申请人： Kai WANG ,  Shile ZHANG ,  David GALAS

    发明人： Kai WANG ,  Shile ZHANG ,  David GALAS

    IPC分类号： C12Q1/68 ,  C40B20/00

    CPC分类号： C12Q1/6851 ,  C12Q1/6883 ,  C12Q2600/178 ,  C40B20/00 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/207

    摘要： Improved methods to quantitate RNA in biological or other analytical samples employ extended RNAs containing adaptors at the 5′ end and polyA sequences coupled to a tag at the 3′ end. The invention method is particularly useful in quantitating microRNAs as primers can be used that need not complement the non-conserved 3′ ends of these molecules.

    摘要翻译： 在生物或其他分析样品中定量RNA的改进方法使用在5'端含有衔接子的延伸RNA和在3'末端与标签偶联的polyA序列。 本发明方法特别可用于定量微小RNA，因为可以使用不需要补充这些分子的非保守3'末端的引物。

    Mass spectrum-based identification and quantitation of proteins and peptides
    68.
    发明授权
    Mass spectrum-based identification and quantitation of proteins and peptides 有权
    基于质谱的蛋白质和肽的鉴定和定量

    公开(公告)号：US08227251B2

    公开(公告)日：2012-07-24

    申请号：US12760500

    申请日：2010-04-14

    申请人： Jeff Ranish ,  Jie Luo ,  Wei Yan

    发明人： Jeff Ranish ,  Jie Luo ,  Wei Yan

    IPC分类号： G01N33/68

    CPC分类号： G01N33/60 ,  Y10T436/101666 ,  Y10T436/13 ,  Y10T436/24

    摘要： The present invention relates to methods for analyzing complex mixtures of proteins, both for identification and quantitation of proteins of interest, and in particular, methods of identification and quantitation of proteins present at low levels, using internal standard peptides isobarically tagged at the N and C termini.

    摘要翻译： 本发明涉及用于分析复合蛋白质混合物的方法，用于鉴定和定量感兴趣的蛋白质，特别是使用在N和C等位标记的内标物质，以低水平存在的蛋白质的鉴定和定量的方法 终点

    Methods for characterizing glycoproteins and generating antibodies for same
    69.
    发明授权
    Methods for characterizing glycoproteins and generating antibodies for same 有权
    表征糖蛋白并产生相同抗体的方法

    公开(公告)号：US08222208B2

    公开(公告)日：2012-07-17

    申请号：US13007393

    申请日：2011-01-14

    申请人： Dan Martin ,  Bernd Wollscheid ,  Rudolf H. Aebersold ,  Julian Watts

    发明人： Dan Martin ,  Bernd Wollscheid ,  Rudolf H. Aebersold ,  Julian Watts

    IPC分类号： A61K38/00 ,  C07K14/00

    CPC分类号： C07K16/28

    摘要： The invention provides methods for preparing a peptide as an immunogen to generate an antibody specific for the deglycosylated form of a glycopolypeptide by preparing a peptide that includes an N-linked glycosylation site of said glycopolypeptide and identifying a peptide sequence that includes said glycosylated site and preparing said peptide wherein N of said site is converted to D.

    摘要翻译： 本发明提供了制备肽作为免疫原的方法，通过制备包含所述糖多肽的N-连接的糖基化位点的肽并鉴定包含所述糖基化位点的肽序列并制备了包含所述糖基化位点的肽序列，从而产生对糖多糖的去糖基化形式特异性的抗体 所述肽，其中所述位点的N转化为D.