公开(公告)号：US20190137447A1

公开(公告)日：2019-05-09

申请号：US16093681

申请日：2017-04-12

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell

IPC: G01N27/62 ,  H01J49/04 ,  H01J49/10 ,  C07B63/00

Abstract: Methods and systems for separating, detecting, and/or quantifying sample enantiomers using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of racemic or non-racemic mixtures of sample enantiomers that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by reacting the sample enantiomers with an enantiomerically-pure compound to form diastereomers separable by DMS.

公开(公告)号：US10256088B2

公开(公告)日：2019-04-09

申请号：US15531505

申请日：2015-10-14

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba

IPC: H01J49/00 ,  H01J49/42

Abstract: An RF voltage is applied across each electrode of a first array of evenly spaced, parallel, and coplanar electrodes and its corresponding electrode of a second array of evenly spaced, parallel, and coplanar electrodes. The RF voltage varies in amplitude according to an RF voltage amplitude gradient. The RF voltage produces an array of different quadrupole RF electric fields in a uniform gap between the first array and the second array. A DC voltage is superimposed on each electrode of the first array and its corresponding electrode of the second array. The DC voltage varies according to a DC voltage gradient in order to produce a DC electric field in the uniform gap. When ions are introduced in the uniform gap, the DC electric field causes the ions to drift toward quadrupole RF electric fields with increasing RF voltage amplitudes where the ions are trapped according to their m/z.

公开(公告)号：US10163617B2

公开(公告)日：2018-12-25

申请号：US15026238

申请日：2014-10-07

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Bruce Andrew Collings

IPC: H01J49/42 ,  H01J49/00

Abstract: Systems and methods are provided for multiplexed precursor ion selection using a filtered noise field (FNF). Two or more different precursor ions are selected using a processor. The processor calculates an FNF waveform. The calculated FNF waveform is applied to a continuous beam of ions using the processor. The processors sends information to a mass spectrometer, which includes an ion source that provides the continuous beam of ions and a first quadrupole that receives the continuous beam of ions, so that the first quadrupole applies the calculated FNF waveform to the continuous beam of ions. The first quadrupole applies the calculated FNF waveform to the continuous beam of ions by applying the calculated FNF waveform between pairs of rods or between pairs of auxiliary electrodes placed between rods.

公开(公告)号：US10128093B2

公开(公告)日：2018-11-13

申请号：US15576874

申请日：2016-05-26

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Stephen A. Tate ,  John Lawrence Campbell

IPC: H01J49/00

Abstract: Systems and methods prevent potentially convolved precursor ion peaks from being excluded in subsequent cycles of an IDA experiment so that additional product ion data is collected. A sample is ionized producing an ion beam. A plurality of cycles of an IDA experiment are performed on the ion beam. During each cycle of the IDA experiment and for each precursor ion peak on a filtered peak list produced in the filtering step of each cycle, several steps are performed. The precursor ion peak is identified in the precursor ion spectrum produced in the MS survey scan step of the cycle. It is determined if the precursor ion peak in the precursor ion spectrum includes a feature of convolution. If the precursor ion peak includes a feature of convolution, the precursor ion peak is prevented from being excluded in a filtering step of one or more subsequent cycles.

公开(公告)号：US10078091B2

公开(公告)日：2018-09-18

申请号：US14401427

申请日：2013-05-17

Applicant: DH Technologies Development Pte. Ltd. ,  Oregon Health and Science University

Inventor： Andrea DeBarber ,  Subhasish Purkayastha ,  Robert D. Steiner ,  Michal Weinstock

IPC: G01N33/00 ,  G01N33/92 ,  G01N33/58 ,  H01J49/00 ,  G01N33/68

CPC classification number: G01N33/92 ,  G01N33/58 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2333/90251 ,  G01N2400/00 ,  G01N2800/04 ,  G01N2800/065 ,  G01N2800/385 ,  H01J49/0031

Abstract: A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the analysis of ketosterol biomarkers such as bile acid precursors from human plasma, serum or whole blood. This method is used for new born screening for Cerebrotendinous Xanthomatosis (CTX). Methods for labeling, analyzing, and quantifying ketosterol biomarkers are also disclosed as are methods that also use mass spectrometry.

公开(公告)号：US20180259481A1

公开(公告)日：2018-09-13

申请号：US15761478

申请日：2016-09-20

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Mark Lies ,  Erika Lin ,  Randy Roushall ,  Marcia Santos

IPC: G01N27/447 ,  B01L3/00

CPC classification number: G01N27/44743 ,  B01L3/502 ,  B01L9/065 ,  B01L2300/0838 ,  B01L2400/0421 ,  G01N27/44791

Abstract: Vials for reducing the volume of sample that must be contained within the vial when interfacing with a variety of analytical instruments are provided herein, and particularly, for use in analytical instruments that interact with a sample via a “polar” or asymmetric probe, including in capillary electrophoresis, liquid chromatography, and gas chromatography, by way of non-limiting example. In accordance with various aspects of the present teachings, the vials can reduce the waste of expensive and/or scarce samples common in the round vials and vial inserts conventionally used in the art.

公开(公告)号：US10062556B2

公开(公告)日：2018-08-28

申请号：US15540750

申请日：2015-12-21

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba

IPC: H01J49/42 ,  H01J49/26 ,  H01J49/00 ,  H01J49/06

CPC classification number: H01J49/0072 ,  H01J49/0054 ,  H01J49/062 ,  H01J49/063

Abstract: A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.

公开(公告)号：US20180240658A1

公开(公告)日：2018-08-23

申请号：US15750550

申请日：2016-08-09

Applicant: DH Technologies Development Pte. Ltd.

Inventor： David Michael Cox ,  Gordana Ivosev

IPC: H01J49/00 ,  H01J49/04 ,  H01J49/26

CPC classification number: H01J49/0031 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/0045 ,  H01J49/04 ,  H01J49/26

Abstract: An m/z range of an ion beam is divided into two or more precursor ion mass selection windows. A pattern of two or more different window m/z ranges to be used during two or more successive cycles for at least one precursor ion mass selection window is determined. The pattern includes an initial window m/z range and one or more successively different window m/z ranges. Each of the one or more successively different window m/z ranges includes at least a portion of the initial window m/z range. A tandem mass spectrometer is instructed to select and fragment the two or more precursor ion mass selection windows during each cycle of a plurality of cycles and to repeatedly use the pattern for each group of two or more successive cycles of the plurality of cycles for the selection and fragmentation of the at least one precursor ion mass selection window.

公开(公告)号：US20180218895A1

公开(公告)日：2018-08-02

申请号：US15107141

申请日：2014-11-28

Applicant: DH Technologies Development PTE Ltd.

Inventor： Bruce Andrew Collings ,  John Vandermey

IPC: H01J49/42 ,  H01J49/00

CPC classification number: H01J49/427 ,  H01J49/0027 ,  H01J49/004 ,  H01J49/4225 ,  H01J49/423

Abstract: A method and apparatus for clearing ions from a multipole ion transmission device which includes introducing a DC or RF clear out pulse to one or more of the rods of the multipole device. The DC pulse is selected so as to supply sufficient kinetic energy to the ions to overcome a pseudo-potential trapping well generated by the RF potentials of the ion transmission device. For an RF pulse, the auxiliary RF signal uses frequencies that correspond to the ejected ion's frequencies of motion. In select embodiments, the multipole device can be a quadrupole or the apparatus can be part of a tandem mass spectrometer.

公开(公告)号：US10001456B2

公开(公告)日：2018-06-19

申请号：US15511712

申请日：2015-08-28

Applicant: DH Technologies Development PTE Ltd.

Inventor： John L Campbell ,  Yves LeBlanc ,  Chang Liu

IPC: C07K1/14 ,  C07K1/00 ,  B01D59/44 ,  B01D15/38 ,  B01D15/36 ,  C12Q1/68 ,  G01N33/53 ,  H01J49/26 ,  G01N27/62 ,  H01J49/04 ,  H01J49/10

CPC classification number: G01N27/624 ,  G01N33/6803 ,  H01J49/04 ,  H01J49/10

Abstract: Methods and systems for separating and/or quantifying peptides using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of one or more peptides, for example, peptides that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by complexing the peptides with a metal cation (e.g., Ca2+) prior to DMS. In some aspects, the present teachings can prevent proton stripping from ionized peptides that can occur in DMS to prevent unintended and/or undesirable alterations to the peptide's charge state distribution.