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公开(公告)号:US09850289B2
公开(公告)日:2017-12-26
申请号:US14775892
申请日:2014-03-17
Applicant: Spogen Biotech Inc.
Inventor: Brian Thompson , Katie Thompson
CPC classification number: C07K14/32 , A01N37/46 , A01N37/48 , A01N63/00 , A01N63/02 , C07K2319/035 , C12N9/0095 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/48 , C12Y118/06001 , C12Y301/01003 , C12Y301/03008 , C12Y302/01004 , C12Y304/00
Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.
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公开(公告)号:US09845342B2
公开(公告)日:2017-12-19
申请号:US14857606
申请日:2015-09-17
Applicant: Spogen Biotech Inc.
Inventor: Brian Thompson , Ashley Siegel
IPC: C07K14/32 , A01N37/44 , A61L2/18 , C09K8/62 , C02F3/34 , E21B43/16 , A23K20/147 , C12P21/02 , C12N1/20 , C12N3/00 , C12N15/62 , C12N15/75 , A01N63/02 , C02F101/10 , C02F101/30 , A23K10/18 , A61K39/00 , C02F103/00 , C02F103/06 , C02F103/10 , C02F103/14 , C02F103/16 , C02F103/26 , C02F103/28
CPC classification number: C07K14/32 , A01N37/44 , A01N63/02 , A23K10/18 , A23K20/147 , A61K39/00 , A61L2/18 , C02F3/342 , C02F3/348 , C02F2101/10 , C02F2101/30 , C02F2103/002 , C02F2103/003 , C02F2103/007 , C02F2103/06 , C02F2103/10 , C02F2103/14 , C02F2103/16 , C02F2103/26 , C02F2103/28 , C07K2319/01 , C07K2319/035 , C07K2319/40 , C09K8/62 , C09K2208/24 , C12N1/20 , C12N3/00 , C12N15/62 , C12N15/75 , C12P21/02 , E21B43/16
Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.
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公开(公告)号:US20170347664A1
公开(公告)日:2017-12-07
申请号:US15461188
申请日:2017-03-16
Applicant: Spogen Biotech Inc.
Inventor: Brian M. Thompson , Ashley Siegel
IPC: A01N63/02 , C12N15/75 , C02F3/34 , A61F13/00 , C12N1/20 , C07K14/32 , A23K10/18 , C12P21/02 , A61L2/18 , C12N3/00 , A61F13/51 , A61K39/00
CPC classification number: A01N63/02 , A23K10/18 , A61F13/00063 , A61F2013/51047 , A61K39/00 , A61K39/0208 , A61K39/07 , A61K2039/523 , A61K2039/552 , A61L2/18 , C02F3/348 , C07K14/32 , C07K14/325 , C07K2319/00 , C12N1/20 , C12N3/00 , C12N15/75 , C12P21/02
Abstract: Fusion proteins, recombinant Bacillus cereus family members that express fusion proteins, and exosporium fragments derived from spores of the recombinant Bacillus cereus family members are provided. Compositions comprising the spores or exosporium fragments are also provided. Methods involving the use of spores of recombinant Bacillus cereus family members and exosporium fragments derived from spores of a recombinant Bacillus cereus family member in the fields of animal health and aquaculture are provided. In particular, methods are for provided for using such spores or exosporium fragments for protecting an animal or an aquatic organism from a pathogen. Methods are also provided for using exosporium fragments for producing an immunogenic response in an aquatic animal. Products for use in protecting animals from pathogens are also provided, including adhesive patches, wound dressings, insert trays for livestock footbaths, hoof bandages, feed, feed additives, and insect foggers.
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公开(公告)号:US20170135353A1
公开(公告)日:2017-05-18
申请号:US15414050
申请日:2017-01-24
Applicant: Spogen Biotech Inc.
Inventor: Brian Thompson , Katie Thompson
CPC classification number: C07K14/32 , A01N37/46 , A01N37/48 , A01N63/00 , A01N63/02 , C07K2319/035 , C12N9/0095 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/48 , C12Y118/06001 , C12Y301/01003 , C12Y301/03008 , C12Y302/01004 , C12Y304/00
Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.
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公开(公告)号:US20140274691A1
公开(公告)日:2014-09-18
申请号:US14213238
申请日:2014-03-14
Applicant: Spogen Biotech Inc.
Inventor: Brian Thompson , Katie Thompson , Brittany Angle
IPC: A01N63/00
CPC classification number: A01N63/00 , A01H3/00 , A01N63/02 , C12R1/01 , C12R1/05 , C12R1/07 , C12R1/075 , C12R1/085 , C12R1/125 , A01N25/00
Abstract: The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.
Abstract translation: 本发明一般涉及植物生长促进细菌新菌株的细菌学纯细菌培养物和包含其的接种物。 本发明还涉及用接种物包被的植物种子,包含接种物的试剂盒和通过将生物学纯细菌培养物或接种物施用于植物,植物种子或植物生长培养基来刺激植物生长的方法。
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