公开(公告)号：US20200020417A1

公开(公告)日：2020-01-16

申请号：US16428656

申请日：2019-05-31

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Mirna Jarosz ,  Serge Saxonov ,  Kevin Ness ,  Benjamin Hindson

IPC: G16B30/00

Abstract: The invention described herein solves challenges in providing a proficient, rapid and meaningful analysis of sequencing data. Methods and computer program products of the invention allow for a system to receive, analyze, and display sequencing data in real-time. The invention provides solutions to several difficulties encountered in assembling short sequencing-reads, and by doing so the invention improves the worth and significance of sequencing data.

公开(公告)号：US20190329245A1

公开(公告)日：2019-10-31

申请号：US16274134

申请日：2019-02-12

Applicant: 10X Genomics, Inc.

Inventor： Donald A. Masquelier ,  Benjamin Hindson ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: B01L3/00 ,  C12Q1/6874 ,  F16K99/00 ,  B01L9/00

Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.

公开(公告)号：US10450607B2

公开(公告)日：2019-10-22

申请号：US16294769

申请日：2019-03-06

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190276817A1

公开(公告)日：2019-09-12

申请号：US16170980

申请日：2018-10-25

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Serge Saxonov ,  Kevin Ness ,  Paul Hardenbol ,  Christopher Hindson ,  Donald Masquelier ,  Mirna Jarosz ,  Michael Schnall-Levin

IPC: C12N15/10 ,  C12Q1/6874 ,  G01N33/543 ,  C12N15/11 ,  C12Q1/6876 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10344329B2

公开(公告)日：2019-07-09

申请号：US16045474

申请日：2018-07-25

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US10337061B2

公开(公告)日：2019-07-02

申请号：US16138448

申请日：2018-09-21

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q1/6804

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20190177789A1

公开(公告)日：2019-06-13

申请号：US16246322

申请日：2019-01-11

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20190176152A1

公开(公告)日：2019-06-13

申请号：US16325922

申请日：2017-08-23

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Tobias Daniel Wheeler ,  Kevin Ness ,  Benjamin Hindson

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F3/0811 ,  B01F13/0086 ,  B01F2215/0037 ,  B01J2/06 ,  B01L2200/0673 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: A microfluidic emulsion droplet generation system and methods of use thereof are provided. The system may include a microfluidic substrate having a flow path configured and arranged for emulsion droplet generation, at least one textured surface in the flow path configured and arranged for inducing surface-mediated coalescence of emulsion droplets; and at least one channel junction in the flow path for emulsion droplet formation.

公开(公告)号：US10245587B2

公开(公告)日：2019-04-02

申请号：US15958391

申请日：2018-04-20

Applicant: 10X Genomics, Inc.

Inventor： Donald A. Masquelier ,  Benjamin Hindson ,  Kevin Ness ,  Rajiv Bharadwaj

IPC: B01L3/00 ,  F16K99/00 ,  B01L9/00 ,  C12Q1/6874

Abstract: An integrated system for processing and preparing samples for analysis may include a microfluidic device including a plurality of parallel channel networks for partitioning the samples including various fluids, and connected to a plurality of inlet and outlet reservoirs, at least a portion of the fluids comprising reagents, a holder including a closeable lid hingedly coupled thereto, in which in a closed configuration, the lid secures the microfluidic device in the holder, and in an open configuration, the lid is a stand orienting the microfluidic device at a desired angle to facilitate recovery of partitions or droplets from the partitioned samples generated within the microfluidic device, and an instrument configured to receive the holder and apply a pressure differential between the plurality of inlet and outlet reservoirs to drive fluid movement within the channel networks.

公开(公告)号：US20190085391A1

公开(公告)日：2019-03-21

申请号：US16165389

申请日：2018-10-19

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Mima Jarosz ,  Paul Hardenbol ,  Michael Schnall-Levin ,  Kevin Ness ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12N15/00 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/683 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2563/179 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/149

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.