摘要:
The invention provides methods for a purifying protein of interest from a mixture comprising the protein of interest and one or more contaminants, including host cell DNA and proteins, by precipitation of the contaminants with caprylic acid. Such methods are particularly useful for purifying antibodies from cell cultures. Moreover, mixtures that have been depleted of contaminants using the methods of the invention can be used directly in downstream chromatography applications (e.g., ion exchange chromatography) without any further purification. These methods lead to manufacturing processes with a minimum number of unit operations and reduce the resource requirements, and thus positively influence the cost of goods for therapeutic protein production.
摘要:
The present invention relates to mobile communication technologies, and discloses a method and a device for handover between User Equipment (UE) in a process of sending a message to enable the after-handover UE receive messages accurately. The method includes: receiving a UE handover request sent by a before-handover UE, wherein the UE handover request carries an identifier of an after-handover UE and indication parameters for sending a message; and sending the message to the after-handover UE according to the identifier of the after-handover UE and the indication parameters for sending the message. The embodiments of the present invention are applied to handover between UEs in the process of sending a message.
摘要:
A transfer notification method, system, and device are provided. The method includes the following steps. A first identifier and a second identifier of a first user are obtained. At least one user in touch with the first user is obtained as a second user. A transfer notification is sent to the second user. The transfer notification includes the first identifier and the second identifier of the first user. Therefore, by using the method for notifying contacts of address book transfer, the contacts in touch with a transferred user can receive a corresponding notification. Therefore, the two parties can update contact data in time and use rich functions provided by a Converged Address Book (CAB) after the transfer.
摘要:
A method for saving instant messages includes: determining a session saving policy; sending the session saving policy to a server; and saving, by the server, instant messages in a session in accordance with the session saving policy. A client, server and system are also provided.
摘要:
The invention is directed to preparing optical elements having a thin, smooth, dense coating or film thereon, and a method for making such coating or film. The coated element has a surface roughness of
摘要:
The invention generally relates to a method for pulmonary delivery of therapeutic, prophylactic and diagnostic agents to a patient wherein the agent is released in a sustained fashion, and to particles suitable for use in the method. In particular, the invention relates to a method for the pulmonary delivery of a therapeutic, prophylactic or diagnostic agent comprising administering to the respiratory tract of a patient in need of treatment, prophylaxis or diagnosis an effective amount of particles comprising a therapeutic, prophylactic or diagnostic agent or any combination thereof in association with a charged lipid, wherein the charged lipid has an overall net charge which is opposite to that of the agent upon association with the agent. Release of the agent from the administered particles occurs in a sustained fashion.
摘要:
A method and apparatus for operating a thread are disclosed. The method includes: receiving a thread operation request that carries a thread operation ID and thread information related operation; and operating a thread according to the operation request. The thread in the embodiments of the present disclosure is independent of the actual content. Therefore, the thread file can be operated according to the requirements of the user, and thus the user experience is improved.
摘要:
A power converter for and method of producing a monotonic rise in output voltage at start-up. In one embodiment, the power converter includes a switch and an error amplifier coupled to an output terminal of the power converter. The power converter also includes a comparator with an output terminal coupled to a control terminal of the switch and an input terminal coupled to an output terminal of the error amplifier configured to enable the switch to conduct for a duty cycle. Additionally, the switch is configured to be turned off at a start-up of the power converter until a voltage of the output terminal of the error amplifier provides a duty cycle for the switch corresponding to an output characteristic pre-charge condition.
摘要:
A method for searching across instant message systems and an interconnecting server are disclosed. The method includes the steps of searching in a SIMPLE IM system by a WV-IMPS user, and searching in a WV-IMPS system by a SIMPLE IM user. The interconnecting server includes a message input/output module and a message processing and converting module. The technical solution of the present invention seamlessly connects the search services of the WV-IMPS instant message system and the SIMPLE instant message system, and thus solves the problem on interconnecting search functions between different types of instant message systems.
摘要:
Mean shift is a nonparametric estimator of density which has been applied to image and video segmentation. Traditional mean shift based segmentation uses a radially symmetric kernel to estimate local density, which is not optimal in view of the often structured nature of image and more particularly video data. The system and method of the invention employs an anisotropic kernel mean shift in which the shape, scale, and orientation of the kernels adapt to the local structure of the image or video. The anisotropic kernel is decomposed to provide handles for modifying the segmentation based on simple heuristics. Experimental results show that the anisotropic kernel mean shift outperforms the original mean shift on image and video segmentation in the following aspects: 1) it gets better results on general images and video in a smoothness sense; 2) the segmented results are more consistent with human visual saliency; and 3) the system and method is robust to initial parameters.