公开(公告)号：US08802921B2

公开(公告)日：2014-08-12

申请号：US13011735

申请日：2011-01-21

Applicant: William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebecca R. Miles ,  Steven R. Webb

Inventor： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebecca R. Miles ,  Steven R. Webb

IPC: C12N15/87 ,  C12N15/82 ,  C12N15/05 ,  C12N5/04 ,  C12N5/10 ,  C07H21/04 ,  A01H5/10 ,  A01H5/00

CPC classification number: C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

Abstract translation: 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

公开(公告)号：US08796510B2

公开(公告)日：2014-08-05

申请号：US13367257

申请日：2012-02-06

Applicant: Jacqueline E. Heard ,  Robert A. Creelman ,  Oliver J. Ratcliffe ,  T. Lynne Reuber ,  James S. Keddie

Inventor： Jacqueline E. Heard ,  Robert A. Creelman ,  Oliver J. Ratcliffe ,  T. Lynne Reuber ,  James S. Keddie

IPC: A01H5/00 ,  A01H5/10 ,  A01H1/00 ,  C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8201 ,  C07K14/415 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8266 ,  C12N15/8267 ,  C12N15/827 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8275 ,  C12N15/8279 ,  C12N15/8282 ,  C12N15/8291 ,  C12N15/8293 ,  Y02A40/146

Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

Abstract translation: 本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生与参照植物相比具有有利特性的转基因植物的方法。 与这些多核苷酸和多肽相关的序列信息也可以用于生物信息学检索方法，并且也被公开。

公开(公告)号：US20140196172A1

公开(公告)日：2014-07-10

申请号：US14129386

申请日：2012-08-02

Applicant: Her Majesty the Queen in Right of Canada, as Repre sented by the Minister of Agriculture and AgriFoo

Inventor： Francois Eudes ,  Trevor Macmillan

IPC: C12N15/82

CPC classification number: C12N15/8201 ,  C07K14/415 ,  C07K2319/07 ,  C07K2319/08 ,  C12N15/8221 ,  C12N15/85

Abstract: Provided are organelle targeting nanocarriers, including peptides, which act to deliver biological molecules such as nucleic acids to non-nuclear organelles such as mitochondria and chloroplasts. Also provided are methods for genetic transformation of non-nuclear organelles using such nanocarriers.

Abstract translation: 提供了靶向纳米载体的细胞器，包括肽，其用于将诸如核酸的生物分子递送到非核细胞器如线粒体和叶绿体。 还提供了使用这种纳米载体的非核细胞器的遗传转化的方法。

公开(公告)号：US20140196169A1

公开(公告)日：2014-07-10

申请号：US14201577

申请日：2014-03-07

Applicant: Kathleen D'HALLUIN ,  Jan DITTGEN ,  Stefan JANSENS ,  Frank MEULEWAETER ,  Koen WETERINGS ,  Hal MOSER ,  Thomas WILDE ,  Ruediger HAIN ,  Udo BICKERS ,  Linda TROLINDER ,  Gary HENNIGER

Inventor： Kathleen D'HALLUIN ,  Jan DITTGEN ,  Stefan JANSENS ,  Frank MEULEWAETER ,  Koen WETERINGS ,  Hal MOSER ,  Thomas WILDE ,  Ruediger HAIN ,  Udo BICKERS ,  Linda TROLINDER ,  Gary HENNIGER

IPC: C12N9/02 ,  C12N15/82

CPC classification number: C12N15/8201 ,  C12N9/0069 ,  C12N15/01 ,  C12N15/8213 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8282 ,  C12N15/8286 ,  C12Y113/11027

Abstract: Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1× of at least one HPPD inhibitor, and methods for making such plants.

Abstract translation: 提供了使用双链DNA断裂诱导酶以目标方式修饰植物基因组的方法和手段，使其非常接近现有的精英事件。 还提供了植物，特别是显示对至少1×的至少一种HPPD抑制剂的野外剂量的耐受性的棉花植物，以及制备这种植物的方法。

公开(公告)号：US20130326645A1

公开(公告)日：2013-12-05

申请号：US13889162

申请日：2013-05-07

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Gregory J. Cost ,  Fyodor Urnov ,  William M. Ainley ,  Joseph F. Petolino ,  Jayakumar Pon Samuel ,  Steven R. Webb ,  Lakshmi Sastry-Dent

IPC: C12N15/85 ,  C12N15/82

CPC classification number: C12N15/8201 ,  C12N15/102 ,  C12N15/8209 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8278 ,  C12N15/8286 ,  C12N15/85 ,  C12N15/87 ,  C12N2800/80 ,  Y02A40/162

Abstract: Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

Abstract translation: 本文公开了用于与供体分子同源性独立的靶向插入细胞基因组的方法和组合物。

公开(公告)号：US20130302804A1

公开(公告)日：2013-11-14

申请号：US13946573

申请日：2013-07-19

Applicant: Seminis Vegetable Seeds, Inc.

Inventor： Joseph Michael PALYS

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12N15/8286 ,  Y02A40/162

Abstract: Methods for identification of successful transformation without a need for in vitro selection are provided. Direct detection of nucleotide sequences of interest is described which eliminate the need for use of ancillary nucleotide sequences.

Abstract translation: 提供了不需要体外选择的用于鉴定成功转化的方法。 描述了目的核苷酸序列的直接检测，其消除了使用辅助核苷酸序列的需要。

公开(公告)号：US08507758B2

公开(公告)日：2013-08-13

申请号：US13335646

申请日：2011-12-22

Applicant: Joseph Michael Palys

Inventor： Joseph Michael Palys

IPC: C12N15/82 ,  C12N15/84 ,  C12N15/10 ,  C12N15/32

CPC classification number: C12Q1/6895 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8265 ,  C12N15/8286 ,  Y02A40/162

Abstract: Methods for identification of successful transformation without a need for in vitro selection are provided. Direct detection of nucleotide sequences of interest is described which eliminate the need for use of ancillary nucleotide sequences.

Abstract translation: 提供了不需要体外选择的用于鉴定成功转化的方法。 描述了目的核苷酸序列的直接检测，其消除了使用辅助核苷酸序列的需要。

公开(公告)号：US20130185830A1

公开(公告)日：2013-07-18

申请号：US13727575

申请日：2012-12-26

Applicant: Monsanto Technology LLC

Inventor： Erik Dersch ,  Richard J. Heinzen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.

公开(公告)号：US20130174299A1

公开(公告)日：2013-07-04

申请号：US13823375

申请日：2011-04-11

Applicant: Akiho Yokota ,  Hiroki Ashida ,  Kinya Akashi ,  Keiichi Ushiyama ,  Shigeru Shigeoka

Inventor： Akiho Yokota ,  Hiroki Ashida ,  Kinya Akashi ,  Keiichi Ushiyama ,  Shigeru Shigeoka

IPC: C12N15/82

CPC classification number: C12N15/8201 ,  C12N15/8261 ,  Y02A40/146

Abstract: The present invention provides: a method for producing stolon-forming plant having an improved tuber production ability as compared with a corresponding wild strain; and a stolon-forming plant produced by the method. The present invention also provides: a method for producing a stolon-forming plant having an improved stolon formation ability as compared with a corresponding wild strain; a stolon-forming plant produced by the method; and a kit for improving a stolon formation ability of a stolon-forming plant. The method of the present invention is a method for producing a stolon-forming plant having an improved tuber production ability as compared with a corresponding wild strain, the method including the step of introducing, into a stolon-forming plant, a Ran gene derived from Citrullus lanatus. The method of the present invention can further include the step of introducing, into a stolon-forming plant, a polynucleotide encoding fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase derived from a blue-green alga.

Abstract translation: 本发明提供：与相应的野生型相比，具有提高的块茎生产能力的sto形成植物的生产方法; 和通过该方法生产的匍匐茎形成植物。 本发明还提供：与相应的野生株相比，具有提高的匍匐茎形成能力的匍匐茎形成植物的制造方法; 通过该方法生产的成sto草植物; 以及用于提高匍匐茎形成植物的匍匐茎形成能力的试剂盒。 本发明的方法是与相应的野生株相比，具有改良的块茎生产能力的sto形成植物的生产方法，所述方法包括以下步骤：向匍匐茎形成植物中导入源自 Citrullus lanatus。 本发明的方法可以进一步包括向成熟匍匐茎植物中导入编码由蓝绿藻衍生的果糖-1,6-二磷酸酶/脱水庚糖-1,7-二磷酸酶的多核苷酸的步骤。

公开(公告)号：US08471114B2

公开(公告)日：2013-06-25

申请号：US12943636

申请日：2010-11-10

Applicant: Teresa Beck Bunn

Inventor： Teresa Beck Bunn

IPC: A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC classification number: A01H5/08 ,  A01G22/00 ,  A01H1/02 ,  C12N5/04 ,  C12N15/8201 ,  C12N15/8241 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N15/8289

Abstract: The invention provides seed and plants of tomato hybrid PX 02470002 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid PX 02470002 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

Abstract translation: 本发明提供番茄混合PX 02470002的种子和植物及其亲本系。 因此，本发明涉及番茄混合PX 02470002及其亲本系的植物，种子和组织培养物，以及通过将这些植物与其他番茄植物如另一种番茄植物杂交而产生的番茄植物的方法 基因型。 本发明还涉及通过这种交叉产生的种子和植物。 本发明还涉及这种植物的部分，包括这种植物的水果和配子。