公开(公告)号：US09610344B2

公开(公告)日：2017-04-04

申请号：US14174635

申请日：2014-02-06

申请人： Virginia Tech Intellectual Properties, Inc.

发明人： Xiang-Jin Meng ,  Nathan M. Beach ,  Sheela Ramamoorthy

IPC分类号： A61K39/12 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/575 ,  A61K2039/70 ,  C12N7/00 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10062

摘要： The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with the PCV2, preferably of subtype PCV2b, capsid gene integrated into a non-pathogenic PCV1 virus genome. In a particular embodiment, the PCV2 capids gene is of subtype PCV2b, the predominant subtype circulating in pigs worldwide. The attenuated chimeric virus, designated PCV1-2b, effectively protects pigs from PCV2b challenges, and can be used as a live vaccine, as well as an inactivated (killed) vaccine, that provides protection and cross protection against PCV2b and PCV2a subtypes infection. The live attenuated vaccine of the present invention is also effective protecting pigs from porcine circovirus-associated disease (PCVAD).

公开(公告)号：US09585951B2

公开(公告)日：2017-03-07

申请号：US13049364

申请日：2011-03-16

申请人： Xiang-Jin Meng ,  Nathan M. Beach ,  Sheela Ramamoorthy

发明人： Xiang-Jin Meng ,  Nathan M. Beach ,  Sheela Ramamoorthy

IPC分类号： A61K39/12 ,  C12N7/00 ,  C12N15/63 ,  A61P31/12 ,  A61P31/20 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/575 ,  A61K2039/70 ,  C12N7/00 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10062

摘要： The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with the PCV2, preferably of subtype PCV2b, capsid gene integrated into a non-pathogenic PCV1 virus genome. In a particular embodiment, the PCV2 capids gene is of subtype PCV2b, the predominant subtype circulating in pigs worldwide. The attenuated chimeric virus, designated PCV1-2b, effectively protects pigs from PCV2b challenges, and can be used as a live vaccine, as well as an inactivated (killed) vaccine, that provides protection and cross protection against PCV2b and PCV2a subtypes infection. The live attenuated vaccine of the present invention is also effective protecting pigs from porcine circovirus-associated disease (PCVAD).

摘要翻译： 本发明提供了一种新型嵌合猪圆环病毒感染性DNA克隆和活体减毒嵌合病毒，其中PCV2优选亚型PCV2b，衣壳基因整合到非致病性PCV1病毒基因组中。 在一个具体实施方案中，PCV2 capids基因是亚型PCV2b，其是在全世界猪中循环的主要亚型。 称为PCV1-2b的减毒嵌合病毒有效保护猪免受PCV2b挑战，可用作活疫苗以及灭活（灭杀）疫苗，可为PCV2b和PCV2a亚型感染提供保护和交叉保护。 本发明的活减毒疫苗也可有效地保护猪免于猪圆环病毒相关疾病（PCVAD）。

公开(公告)号：US20170049875A1

公开(公告)日：2017-02-23

申请号：US15249158

申请日：2016-08-26

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Michael B. Roof ,  Phillip Wayne Hayes ,  Marc Allan Eichmeyer ,  Gregory Paul Nitzel ,  Merrill Lynn Schaeffer

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

摘要： The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and/or multinucleated/giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

摘要翻译： 本发明涉及包含用于治疗几种临床表现（疾病）的猪圆环病毒2型（PCV2）抗原的免疫原性组合物的用途。 优选地，临床表现与PCV2感染相关。 优选地，它们包括淋巴结病，淋巴细胞减少和/或多核/巨细胞组织细胞。 此外，临床症状包括与猪中的一种或多种以下症状相结合的淋巴结病：（1）间质性肺炎与小叶间水肿，（2）皮肤苍白或黄疸，（3）斑驳萎缩性肝脏，（4）胃溃疡 ，（5）肾炎和（6）生殖障碍，例如 流产，死胎，木乃伊等。此外，临床症状包括Pia样病变，通常已知与细胞内劳森氏菌感染有关。

公开(公告)号：US20160258953A1

公开(公告)日：2016-09-08

申请号：US15061690

申请日：2016-03-04

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Arun V. IYER ,  Joseph Ralph HERMANN ,  Michael B. ROOF ,  Eric Martin VAUGHN ,  Merrill Lynn SCHAEFFER

IPC分类号： G01N33/569 ,  C12N7/00 ,  C12P21/00 ,  A61K39/145 ,  C12Q1/70 ,  G01N33/68 ,  C07K14/005 ,  A61K39/12

CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5252 ,  C07K14/005 ,  C07K2319/21 ,  C07K2319/50 ,  C12N7/00 ,  C12N2710/14021 ,  C12N2710/14022 ,  C12N2710/14043 ,  C12N2710/14051 ,  C12N2710/14143 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/20022 ,  C12N2760/20034 ,  C12N2760/20051 ,  C12P21/00 ,  C12Q1/701 ,  G01N33/5091 ,  G01N33/6854 ,  G01N2333/01

摘要： The present invention belongs to the field of compliance markers and marker vaccines which allow for the differentiation between infected and vaccinated individuals. In particular, it relates to a method of determining whether an individual has received an immunogenic composition comprising a recombinant protein produced by a baculovirus expression system in cultured insect cells.

摘要翻译： 本发明属于适应性标记和标记疫苗领域，其允许感染和接种个体之间的分化。 特别地，本发明涉及一种确定个体是否已经接受了包含由培养的昆虫细胞中的杆状病毒表达系统产生的重组蛋白质的免疫原性组合物的方法。

公开(公告)号：US20160193320A1

公开(公告)日：2016-07-07

申请号：US15076381

申请日：2016-03-21

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Michael B. Roof ,  Phillip Wayne Hayes ,  Marc Allan Eichmeyer ,  Gregory Paul Nitzel ,  Merrill Lynn Schaeffer

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

摘要： The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and/or multinucleated/giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

公开(公告)号：US20160090601A1

公开(公告)日：2016-03-31

申请号：US14870985

申请日：2015-09-30

申请人： UNIVERSITY OF THE FREE STATE

发明人： Robert Richard BRAGG ,  Charlotte Enastacia BOUCHER ,  Chrispian William THERON ,  Arina Corli HITZEROTH

IPC分类号： C12N15/81 ,  A23L1/305 ,  C12N7/00 ,  G01N33/68 ,  C07K14/005 ,  A61K39/12

CPC分类号： C07K14/005 ,  A23L33/18 ,  A23V2002/00 ,  A61K38/00 ,  A61K39/12 ,  A61K2039/523 ,  A61K2039/552 ,  C12N7/00 ,  C12N15/815 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  G01N33/56961 ,  G01N33/6854

摘要： The present invention relates to the expression of polypeptides using Yarrowia lipolytica, in particular the secretion of expressed polypeptides into either the extracellular space or the surface of the Y. lipolytica host cell wall. The invention also extends to the use of the polypeptides so expressed in biotechnological applications. The present invention provides an expression construct for the expression of polypeptides using at least a single Yarrowia lipolytica yeast cell, the expression construct having at least one expression cassette, the expression cassette including an acid extracellular protease secretion signal sequence and flanking zeta sequence recombination sites.

摘要翻译： 本发明涉及使用解脂耶氏酵母的多肽的表达，特别是表达的多肽分泌到解脂耶氏酵母宿主细胞壁的细胞外空间或表面中。 本发明还涉及在生物技术应用中如此表达的多肽的用途。 本发明提供使用至少一种解脂耶氏酵母病毒酵母细胞表达多肽的表达构建体，所述表达构建体具有至少一个表达盒，所述表达盒包含酸性细胞外蛋白酶分泌信号序列和侧翼ζ序列重组位点。

公开(公告)号：US09193759B2

公开(公告)日：2015-11-24

申请号：US14176249

申请日：2014-02-10

申请人： Intervet Inc.

发明人： Vicky Fachinger ,  Melanie Sno

IPC分类号： C12P21/06 ,  C07K1/14 ,  C12P21/00 ,  C12N7/00 ,  A61K39/00

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/5258 ,  C07K1/14 ,  C12N2710/14043 ,  C12N2750/10023 ,  C12N2750/10051 ,  C12N2750/14023 ,  C12N2750/14051 ,  C12N2750/14323 ,  C12N2750/14351 ,  C12P21/00

摘要： The present invention relates to methods for the release of baculovirus-expressed Virus-like Particles (VLP's) of non-enveloped viruses from insect cells.

摘要翻译： 本发明涉及从昆虫细胞中释放非包膜病毒的杆状病毒表达的病毒样颗粒（VLP）的方法。

公开(公告)号：US20150190497A1

公开(公告)日：2015-07-09

申请号：US14661689

申请日：2015-03-18

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Marc Eichmeyer ,  Greg Nitzel ,  Merrill Schaeffer

IPC分类号： A61K39/12 ,  G01N33/569 ,  C07K14/005 ,  C12N7/00

CPC分类号： A61K39/12 ,  A61K2039/545 ,  A61K2039/55 ,  A61K2039/552 ,  A61K2039/55511 ,  A61K2039/55566 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034 ,  G01N33/56983

摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

摘要翻译： 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，使病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。

公开(公告)号：US09011872B2

公开(公告)日：2015-04-21

申请号：US13728228

申请日：2012-12-27

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Marc Allan Eichmeyer ,  Gregory Paul Nitzel ,  Merrill Lynn Schaeffer

IPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  C07K14/005 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

摘要翻译： 提供了一种用于从猪圆环病毒2型开放阅读框架2回收蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中所含的细胞的步骤，使病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取程序。

公开(公告)号：US20140220067A1

公开(公告)日：2014-08-07

申请号：US14174635

申请日：2014-02-06

申请人： Virginia Tech Intellectual Properties, Inc.

发明人： Xiang-Jin MENG ,  Nathan M. Beach ,  Sheela Ramamoorthy

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/575 ,  A61K2039/70 ,  C12N7/00 ,  C12N2750/10021 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10062

摘要： The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with the PCV2, preferably of subtype PCV2b, capsid gene integrated into a non-pathogenic PCV1 virus genome. In a particular embodiment, the PCV2 capids gene is of subtype PCV2b, the predominant subtype circulating in pigs worldwide. The attenuated chimeric virus, designated PCV1-2b, effectively protects pigs from PCV2b challenges, and can be used as a live vaccine, as well as an inactivated (killed) vaccine, that provides protection and cross protection against PCV2b and PCV2a subtypes infection. The live attenuated vaccine of the present invention is also effective protecting pigs from porcine circovirus-associated disease (PCVAD).

摘要翻译： 本发明提供了一种新型嵌合猪圆环病毒感染性DNA克隆和活体减毒嵌合病毒，其中PCV2优选亚型PCV2b，衣壳基因整合到非致病性PCV1病毒基因组中。 在一个具体实施方案中，PCV2 capids基因是亚型PCV2b，其是在全世界猪中循环的主要亚型。 称为PCV1-2b的减毒嵌合病毒有效保护猪免受PCV2b挑战，可用作活疫苗以及灭活（灭杀）疫苗，可为PCV2b和PCV2a亚型感染提供保护和交叉保护。 本发明的活减毒疫苗也可有效地保护猪免于猪圆环病毒相关疾病（PCVAD）。